Cardiac electrophysiology evaluation system for improved drug assessment
Project/Area Number |
22K12801
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 90110:Biomedical engineering-related
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Research Institution | Osaka University |
Principal Investigator |
李 俊君 大阪大学, 大学院医学系研究科, 特任助教 (10723786)
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Co-Investigator(Kenkyū-buntansha) |
劉 莉 大阪大学, 大学院医学系研究科, 特任准教授 (50380093)
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Project Period (FY) |
2022-04-01 – 2025-03-31
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Project Status |
Granted (Fiscal Year 2022)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2024: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | Pluripotent stem cells / Cardiomyocytes / Maturation / Drug assessment / drug assessment / cardiomyocytes / maturation / pluripotent stem cells |
Outline of Research at the Start |
ヒトiPS細胞由来の心筋細胞は再生医療への応用だけではなく、創薬や薬剤毒性評価への応用にも期待されている。しかし、既存の心筋細胞分化誘導法で得られた心筋細胞はまだ未熟であるため、創薬への応用に十分満たされていない。本研究課題において、自発循環進行波を利用して、未熟な心筋細胞の成熟度を加速させ、最終的に成人と近いレベルの心筋細胞を獲得し、心筋毒性評価や創薬への応用を実現することを目指している。さらに、ドラックスクリーニングの応用に合わせ、自発性循環進行波現象を誘導するデバイスを融合したMulti-wells多電極システムを開発し、ハイスループットスクリーニング可能なシステムの開発を目指す。
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Outline of Annual Research Achievements |
We planned to reproduce the traveling wave on 2D plate and optimize the best condition for obtain matured hiPSC derived cardiomyocytes, to compare the traveling wave matured cardiomyocytes with adult cells. The achievements in the year include: 1. The traveling wave has been generated in cardiac tissue cultured on both plate and nanofiber sheets. The electrical signal of cardiac tissue could be recorded by using MEA. 2.The maturity of traveling wave matured cardiomyocytes has been confirmed by using staining, TEM, RNAsequencing and other methods. Their maturation level has been compared with those from human adult. 3. We published 2 papers and gave an oral presentation in 87th Annual Scientific Meeting of the Japanese Circulation Society, and obtained the young investigator award.
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Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have accomplished the objective of the first year rather smoothly. Now we have already been working according to second year plan.
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Strategy for Future Research Activity |
In this year, we will focus on integrating the traveling wave matured cardiac tissue on a multi electrode array (MEA) system. The drug test will also be performed on the matured tissue with MEA system. The tissue culture method and the drug test condition will be optimized. The data collected during experiment will be summarized to prepare papers.
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Report
(1 results)
Research Products
(3 results)
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[Presentation] Human iPSC-derived closed-loop cardiac tissue for improved drug assessment2023
Author(s)
Ying Hua, Junjun Li, Xiang Qu, Jingbo Zhang, Masako Ishida, Noriko Yoshida, Hayato Miyoshi, Mikio Shiba3, Shuichiro Higo4,5, Nagako Sougawa1, Maki Takeda1, Takuji Kawamura1, Ryohei Matsuura, Toshihiko Toyofuku, Yoshiki Sawa, Li Liu and Shigeru Miyagawa
Organizer
The 87th Annual Scientific Meeting of the Japanese Circulation Society (JCS2023)
Related Report
Invited