• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Stop-codon readthroug provides novel functions for Syntaxin17

Research Project

Project/Area Number 22K15099
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44010:Cell biology-related
Research InstitutionTokyo Institute of Technology

Principal Investigator

KAWAGUCHI Kohei  東京工業大学, 科学技術創成研究院, 特任助教 (10835515)

Project Period (FY) 2022-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2022: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsショウジョウバエ / ストップコドンの読み飛ばし / Stx17 / 膜輸送 / オートファジー / オルガネラ / SNARE
Outline of Research at the Start

ショウジョウバエを始めとした昆虫では、他の生物と比較して、ストップコドンの読み飛ばしが高頻度に見られるが、その具体的な意義はこれまで明らかにされていない。 本研究は、ショウジョウバエを用いて、ストップコドンの読み飛ばしにより生じたSNAREタンパク質Stx17の新たなアイソフォームが、どのような生理的機能をもつのか明らかにする。本研究から得られる知見は、ストップコドン読み飛ばしがタンパク質の機能を変化させることを示す初めての例となり、当該研究領域の発展に大きく寄与すると期待される。

Outline of Final Research Achievements

Using the assay system we developed, we revealed that the elongated form of Stx17 is highly expressed in neural cells. Additionally, we discovered that the elongated form of Stx17 is more frequently localized in the endoplasmic reticulum, Golgi apparatus, and mitochondria compared to the regular form across various tissues. Furthermore, we found that the elongated form of Stx17 binds with different SNARE partners than the regular form. Unfortunately, we could not identify any clear phenotypes from the elongated Stx17-specific knockout strains. Moving forward, we plan to conduct behavioral analyses and other investigations.

Academic Significance and Societal Importance of the Research Achievements

遺伝性疾患の数割がナンセンス変異と言われており、ストップコドンの読み飛ばしは多くの疾患の治療戦略として有望である。本研究は、ストップコドンの読み飛ばしが高頻度で起こるショウジョウバエをモデルにすることでメカニズムの一端を明らかにした。この成果は、様々な疾患の治療戦略を生み出す重要な基盤となりうるものである。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (4 results)

All 2024 2023 2022

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (1 results) (of which Invited: 1 results)

  • [Journal Article] PIGB maintains nuclear lamina organization in skeletal muscle of Drosophila2024

    • Author(s)
      Yamamoto-Hino Miki、Ariura Masaru、Tanaka Masahito、Iwasaki Yuka W.、Kawaguchi Kohei、Shimamoto Yuta、Goto Satoshi
    • Journal Title

      Journal of Cell Biology

      Volume: 223 Issue: 2 Pages: 1-30

    • DOI

      10.1083/jcb.202301062

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shaping transverse-tubules: central mechanisms that play a role in the cytosol zoning for muscle contraction2023

    • Author(s)
      Kawaguchi Kohei、Fujita Naonobu
    • Journal Title

      The Journal of Biochemistry

      Volume: 175 Issue: 2 Pages: 125-131

    • DOI

      10.1093/jb/mvad083

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A <i>Drosophila</i> toolkit for HA-tagged proteins unveils a block in autophagy flux in the last instar larval fat body2022

    • Author(s)
      Murakawa Tadayoshi、Nakamura Tsuyoshi、Kawaguchi Kohei、Murayama Futoshi、Zhao Ning、Stasevich Timothy J.、Kimura Hiroshi、Fujita Naonobu
    • Journal Title

      Development

      Volume: 149 Issue: 6 Pages: 1-12

    • DOI

      10.1242/dev.200243

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 近接依存性標識法による 筋細胞特異的な膜構造体・T管のin vivoプロテオミクス2022

    • Author(s)
      川口紘平, 中村毅, 村川直柔, 小泉美智子, 吉川治孝, 小迫英尊, 藤田尚信
    • Organizer
      第95回日本生化学会大会
    • Related Report
      2022 Research-status Report
    • Invited

URL: 

Published: 2022-04-19   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi