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Mechanism elucidation of diabetic kidney glomerular lesion based on single-cell RNA sequence.

Research Project

Project/Area Number 22K16223
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 53040:Nephrology-related
Research InstitutionKumamoto University

Principal Investigator

Fujimoto Daisuke  熊本大学, 大学院生命科学研究部(医), 助教 (00896649)

Project Period (FY) 2022-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2022: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords糖尿病性腎臓病 / 糸球体 / シングルセル解析 / 細胞連関 / ポドサイト / メサンギウム細胞
Outline of Research at the Start

本邦において糖尿病性腎臓病(DKD)は透析導入原疾患の第1位を占め続けているにも関わらず、治療満足度・治療貢献度の依然低い疾患である。また病態進展の詳細な機序も十分に解明されているとは言い難い。本研究はこれまで技術的に限界が大きかったDKDに対するシングルセル解析を質の高いレベルで確立することで病態を解明し、従来とは異なる機序に着目した新規治療の開発に繋げることを主目的とする。

Outline of Final Research Achievements

This study aims to elucidate the mechanisms of diabetic kidney disease (DKD) based on glomerular single-cell RNA sequencing database. Our original single-cell protocol enabled us to explore several candidate genes which may be involved in DKD pathogenesis. We cretaed genetically modified animals and evaluated the renal phenotype, which could lead to the development of novel therapeutic strategy against DKD.

Academic Significance and Societal Importance of the Research Achievements

糖尿病性腎臓病(DKD)は、末期腎不全に至る原因疾患として最多であり、根本的な治療法にも乏しいのが実情である。本研究はシングルセル解析により糸球体構成細胞種毎の解析が可能となり、病変形成に関与する遺伝子・パスウェイの探索及びそれらを標的としたDKD新規治療法の開発へ向けて大きな足がガリとなることが期待される。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (6 results)

All 2023 2022 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Need to continue or discontinue RAS inhibitors as CKD stage advances? Any alternative?2023

    • Author(s)
      Fujimoto Daisuke、Kuwabara Takashige、Mukoyama Masashi
    • Journal Title

      Hypertension Research

      Volume: 46 Issue: 8 Pages: 2048-2050

    • DOI

      10.1038/s41440-023-01318-7

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel VCP modulator KUS121 exerts renoprotective effects in ischemia-reperfusion injury with retaining ATP and restoring ERAD-processing capacity.2022

    • Author(s)
      Hata Yusuke、Date Ryosuke、Fujimoto Daisuke、Ikeda Hanako Ohashi、Umemoto Shuro、Kanki Tomoko、Nishiguchi Yoshihiko、Mizumoto Teruhiko、Hayata Manabu、Kakizoe Yutaka、Izumi Yuichiro、Kakizuka Akira、Mukoyama Masashi、Kuwabara Takashige
    • Journal Title

      American Journal of Physiology-Renal Physiology

      Volume: - Issue: 5 Pages: F577-F586

    • DOI

      10.1152/ajprenal.00392.2021

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Osteocrin, a bone-derived humoral factor, exerts a renoprotective role in ischemia?reperfusion injury in mice2022

    • Author(s)
      Nishiguchi Yoshihiko、Hata Yusuke、Date Ryosuke、Fujimoto Daisuke、Umemoto Shuro、Kanki Tomoko、Yokoi Hideki、Mori Keita P、Handa Takaya、Watanabe-Takano Haruko、Kanai Yugo、Yasoda Akihiro、Izumi Yuichiro、Kakizoe Yutaka、Mochizuki Naoki、Mukoyama Masashi、Kuwabara Takashige
    • Journal Title

      Nephrology Dialysis Transplantation

      Volume: 37 Pages: 444-453

    • Related Report
      2022 Research-status Report
  • [Presentation] エクソソーム介在性糸球体内細胞間クロストークを標的とした新たな糖尿病性腎臓病治療薬の探索2023

    • Author(s)
      藤本大介
    • Organizer
      第45回日本高血圧学会学術総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Novel drug screening strategy to explore exosome-targeted intervention of intraglomerular crosstalk for diabetic kidney disease2023

    • Author(s)
      Fujimoto Daisuke
    • Organizer
      ASN KidneyWeek 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 熊本大学腎臓内科

    • URL

      https://www.kumadai-nephrology.com/

    • Related Report
      2023 Annual Research Report

URL: 

Published: 2022-04-19   Modified: 2025-01-30  

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