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Identification of factors endowing the genome with a high plasticity in the mouse and their applications to biomedical researches

Research Project

Project/Area Number 23220011
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Ogura Atsuo  国立研究開発法人理化学研究所, バイオリソースセンター, 室長 (20194524)

Co-Investigator(Kenkyū-buntansha) 阿部 訓也  国立研究開発法人理化学研究所, バイオリソースセンター, チームリーダー (40240915)
石野 史敏  東京医科歯科大学, 難治疾患研究所, 教授 (60159754)
若菜 茂晴  国立研究開発法人理化学研究所, バイオリソースセンター, チームリーダー (90192434)
Co-Investigator(Renkei-kenkyūsha) SANKAI Tadashi  国立研究開発法人医薬基盤・健康・栄養研究所, 霊長類医科学研究センター, 主任研究員 (80300937)
NAGAI Takashi  国立研究開発法人農業・食品産業技術総合機構, 領域長 (20391378)
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥206,180,000 (Direct Cost: ¥158,600,000、Indirect Cost: ¥47,580,000)
Fiscal Year 2015: ¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2014: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000)
Fiscal Year 2013: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000)
Fiscal Year 2012: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000)
Fiscal Year 2011: ¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000)
Keywordsゲノム再プログラム化 / ゲノム可塑性 / 核移植クローン / マウス / ES細胞
Outline of Final Research Achievements

The present study was undertaken to identify the factor(s) responsible for the genomic plasticity, which is specifically observed in the genome of the 129 strain mice. First, we employed the forward genetics approach, using B6 x 129 recombinant inbred (RI) strains. Based on the cloning efficiencies and the placental phenotypes observed from 9 RI strains and the polymorphic information of the RI strains, we identified four candidate genomic regions for the plasticity factor(s). We then cloned several consomic strains and successfully narrowed down the position to chromosome 8. According to the B6-129 polymorphism, expression patterns, and functions, we identified one epigenetics-related gene. Finally, we successfully generated BAC transgenic mice that carried the entire region of the gene from the 129 genome, which we expect to explore the mechanism of the genomic plasticity imposed on the 129 mouse genome.

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(9 results)
  • 2016 Research Progress Assessment (Verification Result) ( PDF )
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (44 results)

All 2016 2015 2014 2013 2012 2011 Other

All Journal Article (22 results) (of which Peer Reviewed: 22 results,  Open Access: 11 results,  Acknowledgement Compliant: 9 results) Presentation (14 results) (of which Int'l Joint Research: 4 results,  Invited: 13 results) Book (5 results) Remarks (3 results)

  • [Journal Article] Cellular dynamics of mouse trophoblast stem cells: Identification of a persistent stem cell type.2016

    • Author(s)
      Motomura K, Oikawa M, Hirose M, Honda A, Togayachi S, Miyoshi H, Ohinata Y, Sugimoto M, Abe K, Inoue K, Ogura A.
    • Journal Title

      Biology of Reproduction

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Selection of accurate reference genes in mouse trophoblast stem cells for reverse transcription-quantitative polymerase chain reaction2016

    • Author(s)
      Motomura K, Inoue K, Ogura A.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 62 Issue: 3 Pages: 311-315

    • DOI

      10.1262/jrd.2015-170

    • NAID

      130005157175

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] High-Yield Superovulation in Adult Mice by Anti-Inhibin Serum Treatment Combined with Estrous Cycle Synchronization2016

    • Author(s)
      Ayumi Hasegawa, Keiji Mochida, Hiroki Inoue, Yoshihiro Noda, Tamao Endo, Gen Watanabe, Atsuo Ogura
    • Journal Title

      BIOLOGY OF REPRODUCTION

      Volume: 94 Issue: 1 Pages: 1-8

    • DOI

      10.1095/biolreprod.115.134023

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposon.2015

    • Author(s)
      Hatanaka Y, Inoue K, Oikawa M, Kamimura S, Ogonuki N, Kodam E, Ohkawa Y, Tsukada Y, Ogura A.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 112 Pages: 14641-14646

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In quest of genomic treasure2015

    • Author(s)
      Inoue K, Ogura A.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 61 Issue: 6 Pages: 489-493

    • DOI

      10.1262/jrd.2015-098

    • NAID

      130005113565

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Trichostatin A specifically improves the aberrant expression of transcription factor genes in embryos produced by somatic cell nuclear transfer.2015

    • Author(s)
      Inoue K, Oikawa M, Kamimura S, Ogonuki N, Nakamura T, Nakano T, Abe K, Ogura A.
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 10127-10127

    • DOI

      10.1038/srep10127

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impaired active DNA demethylation in zygotes generated by round spermatid injection.2015

    • Author(s)
      Kurotaki YK, Hatanaka Y, Kamimura S, Oikawa M, Inoue H, Ogonuki N, Inoue K, Ogura A
    • Journal Title

      Human Reproduction

      Volume: 30 Issue: 5 Pages: 1178-1187

    • DOI

      10.1093/humrep/dev039

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Generation of cloned mice from adult neurons by direct nuclear transfer.2015

    • Author(s)
      Mizutani E, Oikawa M, Kassai H, Inoue K, Shiura H, Hirasawa R, Kamimura S, Matoba S, Ogonuki N, Nagatomo H, Abe K, Wakayama T, Aiba A, Ogura A
    • Journal Title

      Biology of Reproduction

      Volume: 92 Issue: 3 Pages: 1-11

    • DOI

      10.1095/biolreprod.114.123455

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Devising assisted reproductive technologies for wild-derived strains of mice: 37 strains from five subspecies of Mus musculus.2014

    • Author(s)
      Mochida K, Hasegawa A, Otaka N, Hama D, Furuya T, Yamaguchi M, Ichikawa E, Ijuin M, Taguma K, Hashimoto M, Takashima R, Kadota M, Hiraiwa N, Mekada K, Yoshiki A, Ogura A
    • Journal Title

      PLoS One

      Volume: 9 Issue: 12 Pages: e114305-e114305

    • DOI

      10.1371/journal.pone.0114305

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Establishment of paternal genomic imprinting in mouse prospermatogonia analyzed by nuclear transfer.2014

    • Author(s)
      Kamimura S, Hatanaka, Y, Hirasawa,R Matsumoto K, Oikawa, M, Lee J, Matoba S, Mizutani E, Ogonuki N, Inoue K, Kohda T, Ishino F, Ogura A
    • Journal Title

      Biology of Reproduction

      Volume: 91 Issue: 5 Pages: 1-12

    • DOI

      10.1095/biolreprod.114.120451

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Understanding the X chromosome inactivation cycle in mice: A comprehensive view provided by nuclear transfer.2014

    • Author(s)
      Oikawa M, Inoue K, Shiura H, Matoba S, Kamimura S, Hirose M, Mekada K, Yoshiki A, Tanaka A, Abe K, Ishino F, Ogura A.
    • Journal Title

      Epigenetics

      Volume: 9 Issue: 2 Pages: 204-211

    • DOI

      10.4161/epi.26939

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Somatic donor cell type correlates with embryonic, but not extra-embryonic, gene expression in postimplantation cloned embryos.2013

    • Author(s)
      Hirasawa R, Matoba S, Inoue K, Ogura A
    • Journal Title

      PLoS One

      Volume: 8 Issue: 10 Pages: e76422-e76422

    • DOI

      10.1371/journal.pone.0076422

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Naive-like conversion overcomes the limited differentiation capacity of induced pluripotent stem cells.2013

    • Author(s)
      Honda A, Hatori M, Hirose M, Honda C, Izu H, Inoue K, Hirasawa R, Matoba S, Togayachi S, Miyoshi H, Ogura A
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Issue: 36 Pages: 26157-26166

    • DOI

      10.1074/jbc.m113.502492

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mouse cloning using a drop of peripheral blood.2013

    • Author(s)
      Kamimura S, Inoue K, Ogonuki N, Hirose M, Oikawa M, Yo M, Ohara O, Miyoshi H, Ogura A
    • Journal Title

      Biology of Reproduction

      Volume: 89 Issue: 2 Pages: 1-6

    • DOI

      10.1095/biolreprod.113.110098

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent advancements in cloning by somatic cell nuclear transfer.2013

    • Author(s)
      Ogura, et al.
    • Journal Title

      Philos Trans R Soc Lond B Biol Sci

      Volume: 368 Issue: 1609 Pages: 20110329-20110329

    • DOI

      10.1098/rstb.2011.0329

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High osmolality vitrification : A new method for the simple and temperature-permissive cryopreservation of mouse embryos.2013

    • Author(s)
      Mochida, et al.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 1 Pages: e49316-e49316

    • DOI

      10.1371/journal.pone.0049316

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient production of offspring from Japanese wild-derived strains of mice (Mus musculus molossinus) by improved assisted reproductive technologies2012

    • Author(s)
      Hasegawa A
    • Journal Title

      Biology of Reproduction

      Volume: 86 Issue: 5 Pages: 1-7

    • DOI

      10.1095/biolreprod.111.098491

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5MeC to 5HmC in early embryos2012

    • Author(s)
      Nakamura, T., Liu, Y.U., Nakashima, H., Umehara, H., Inoue, K., Matoba, S., Tachibana, M., Ogura, A., Shinkai, Y., Nakano, T
    • Journal Title

      Nature

      Volume: 486 Issue: 7403 Pages: 415-419

    • DOI

      10.1038/nature11093

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution of mouse spermatogonial stem cell niches in culture2012

    • Author(s)
      Kanatsu-Shinohara, M., Inoue, K., Takashima, S.,Takehashi, M.,Ogonuki, N., Morimoto, H., Nagasawa, T., Ogura, A. and Shinohara, T
    • Journal Title

      Cell Stem Cell

      Volume: 11(4) Issue: 4 Pages: 567-578

    • DOI

      10.1016/j.stem.2012.06.011

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of mouse embryonic stem cell lines from maturing oocytes by direct conversion of meiosis into mitosis2011

    • Author(s)
      Fulka H, et al
    • Journal Title

      Stem Cells

      Volume: 29 Issue: 3 Pages: 517-527

    • DOI

      10.1002/stem.585

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro production of functional sperm in cultured neonatal mouse testes2011

    • Author(s)
      T. Sato, K. Katagiri, A. Gohbara, K. Inoue, N. Ogonuki, A. Ogura, Y. Kubota, T. Ogawa
    • Journal Title

      Nature

      Volume: 471 Issue: 7339 Pages: 504-507

    • DOI

      10.1038/nature09850

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos2011

    • Author(s)
      Matoba S, et al
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108 Issue: 51 Pages: 20621-20626

    • DOI

      10.1073/pnas.1112664108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Nuclear transfer for the study of developmental epigenetics2016

    • Author(s)
      Ogura A
    • Organizer
      International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming
    • Place of Presentation
      Yamanashi University, Chuo-shi, Yamanashi
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Epigenetic dynamics in embryos during the periimplantation period: comparison of fertilization-derived and SCNT-derived embryos2016

    • Author(s)
      Ogura A
    • Organizer
      International Symposium on Epigenome Dynamics and Regulation in Germ Cells
    • Place of Presentation
      Kyoto University, Kyoto-shi, Kyoto
    • Year and Date
      2016-02-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nuclear transfer in the study of developmental epigenetic2016

    • Author(s)
      Ogura A
    • Organizer
      International Symposium for RIKEN Epigenetics Program 2016
    • Place of Presentation
      RIKEN Wako, Wako-shi, Saitama
    • Year and Date
      2016-02-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of reproductive engineering techniques in mice2016

    • Author(s)
      Ogura A
    • Organizer
      The 3rd Symposium of Thai Society for Animal Reproduction
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2016-01-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Epigenetic abnormalities associated with somatic cell nuclear transfer in mice2014

    • Author(s)
      Ogura, A
    • Organizer
      The 37th Annual Meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      神奈川県横浜市、パシフィコ横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Analysis of the Dynamics of X Chromosome Inactivation by Nuclear Transfer2013

    • Author(s)
      Ogura A
    • Organizer
      The 61st NIBB Conference "Cellular Community in Mammalian Embryogenesis”
    • Place of Presentation
      愛知県岡崎市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 核移植技術により明らかにされる哺乳類の発生エピジェネティクス2013

    • Author(s)
      小倉淳郎
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 体細胞核移植クローンの効率を決定する因子の探索2013

    • Author(s)
      井上貴美子、小倉淳郎
    • Organizer
      第84回遺伝学会
    • Place of Presentation
      神奈川県横浜市慶応技術大学日吉キャンパス
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ICSI and its practical applications2012

    • Author(s)
      Ogura A
    • Organizer
      EMMA Cryopreservation Workshop
    • Place of Presentation
      Madrid, Spain
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] X-chromosome inactivation status in cloned mouse embryos2012

    • Author(s)
      Ogura A
    • Organizer
      "Epigenetic Memory" The Company of Biologists Workshop
    • Place of Presentation
      Steyning, UK
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Nuclear transfer for the study of X chromosome inactivation in mice2012

    • Author(s)
      Ogura A
    • Organizer
      Mouse Molecular Genetics
    • Place of Presentation
      Calfornia, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Analysis of the dynamics of imprinted X chromosome inactivation in mice by nuclear transfer2012

    • Author(s)
      Ogura A
    • Organizer
      The 9th Annual Conference of the Asian Reproductive Biotechnology Society
    • Place of Presentation
      Fukuoka
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Nuclear transfer for the study of epigenetic dynamics in mammalian development2012

    • Author(s)
      Ogura A
    • Organizer
      The 84th Annual Meeting of the Genetics Society of Japan
    • Place of Presentation
      Manila, Philippines
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 研究を支えるマウスの発生工学技術開発:最近の核移植,顕微授精,幹細胞,凍結技術について2011

    • Author(s)
      小倉淳郎
    • Organizer
      第25回日本糖尿病・肥満動物学会
    • Place of Presentation
      東京大学(東京)(招待講演)
    • Year and Date
      2011-11-05
    • Related Report
      2011 Annual Research Report
  • [Book] 細胞工学 34(7) 特集エピゲノム制御の最新メカニズム2015

    • Author(s)
      井上貴美子、小倉淳郎
    • Publisher
      秀潤社
    • Related Report
      2015 Annual Research Report
  • [Book] 哺乳動物の発生工学2014

    • Author(s)
      小倉淳郎
    • Total Pages
      200
    • Publisher
      朝倉書店
    • Related Report
      2013 Annual Research Report
  • [Book] Principle of Cloning, 2nd Ed.2013

    • Author(s)
      Inoue K, Ogura A
    • Total Pages
      585
    • Publisher
      Elsevia
    • Related Report
      2013 Annual Research Report
  • [Book] 繁殖生物学2013

    • Author(s)
      小倉淳郎
    • Total Pages
      313
    • Publisher
      インターズー
    • Related Report
      2013 Annual Research Report
  • [Book] 卵子学2011

    • Author(s)
      小倉淳郎
    • Publisher
      京都大学学術出版会
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://ja.brc.riken.jp/lab/kougaku/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 遺伝工学基盤技術室

    • URL

      http://www.brc.riken.go.jp/lab/kougaku/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.brc.riken.go.jp/lab/kougaku/

    • Related Report
      2011 Annual Research Report

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Published: 2011-06-18   Modified: 2019-07-29  

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