| Project/Area Number |
23240062
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
WAKANA Shigeharu 独立行政法人理化学研究所, バイオリソースセンター, チームリーダー (90192434)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Takeo 山梨大学, 医学工学総合研究部, 教授 (70293511)
FURUICHI Teiichi 東京理科大学, 理工学部応用生物学科, 教授 (50219094)
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| Project Period (FY) |
2011-05-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000)
Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2012: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2011: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000)
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| Keywords | DOHaD / 発達障害 / 行動解析 / 実験動物学 / 胎児期栄養 / CAPSタンパク質 / 葉酸 / 遺伝子発現 / マウス / 胎生妊娠期低栄養 / エピジェネティクス / 発達障害モデル / エピゲノム / モデルマウス |
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| Research Abstract |
The Developmental Origins of Health and Disease:DOHaD paradigm is a concept that in utero experiences reprogram an individual for later-in-life risk of diseases. In this study, we conducted experiments to validate the DOHaD theory in the developmental disorder with well-controlled experimental procedures using mouse model. We provided AIN93G as control diet and low-protein diet (LP) to pre-pregnant and pregnant females. After birth, behavioral phenotypes of offspring, gene expression level in brain, and genomic methylation levels in promoters of genes were examined. The offspring which were exposed to malnutrition in utero exhibited increased activity in the home cage, decreased contact to novel object, and decreased social investigation. The adult offspring of LP group group exhibited different pattern of mRNA expression and genomic methylation pattern relative to AIN93G group. However, no direct interaction between expression levels and methylation levels of genes were detected.
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