Project/Area Number |
23241075
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Chemical biology
|
Research Institution | Keio University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
MATSUBARA Teruhiko 慶應義塾大学, 理工学部, 講師 (10325251)
|
Co-Investigator(Renkei-kenkyūsha) |
SAKAKIBARA Yasubumi 慶應義塾大学, 理工学部, 教授 (10287427)
Fujimoto Junichiro 独立行政法人国立成育医療研究センター, 臨床研究センター, センター長 (60175578)
Umezawa Akihiro 独立行政法人国立成育医療研究センター, 生殖医療研究部, 部長 (70213486)
SUZUKI Tetsuro 浜松医科大学, 医学部, 教授 (00250184)
|
Project Period (FY) |
2011-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥32,760,000 (Direct Cost: ¥25,200,000、Indirect Cost: ¥7,560,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2014: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2013: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
|
Keywords | グライコミクス / 糖鎖工学 / 糖鎖プライマー / 質量分析装置 / がん細胞 / インフルエンザウイルス / C型肝炎ウイルス / iPS細胞 / 糖鎖ライブラリー / ムコ多糖症 / 腫瘍マーカー / オリゴ糖 / 転移性がん細胞 / O-グリカン / グリコサミノグリカン / 糖鎖 / 糖脂質 / Oーグリカン / 糖鎖解析 |
Outline of Final Research Achievements |
In the present study, we carried out the comparative glycan analysis between cells using saccharide primer method. By the comparative analysis using saccharide primers, glycans contributed to metastasis of osteosarcoma cells or pulmonary adenocarcinoma cells and replication of genome of hepatitis C virus in cells were identified. By the overexpression or knockdown of glycosyltransferases related to the biosynthesis of the glycans, the cell migration and replication of HCV genome were successfully suppressed. Furthermore, using saccharide libraries obtained by saccharide primer method, detection method of influenza virus and substrates for neonatal mass screening of mucopolysaccharidosis were developed. By the progress of glycan technology, the evolution toward novel diagnostic technique, quality assessment of iPS cells, mechanism analysis of disease related with glycan, development of novel drug delivery systems, and control technique of cell function was achieved.
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