Development of novel luciferin analogues for utilizing luciferases as new functional tools in vivo
| Project/Area Number |
23249004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
URANO Yasuteru 東京大学, 医学(系)研究科(研究院), 教授 (20292956)
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| Project Period (FY) |
2011-05-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
Fiscal Year 2013: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2012: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
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| Keywords | 生物発光 / ルシフェラーゼ / ルシフェリン / 電子移動 / 細胞膜透過性 / 一酸化窒素 / 活性酸素 / プローブ / エネルギー共鳴移動 / 近赤外蛍光 / 光増感剤 / インビボ |
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| Outline of Final Research Achievements |
Live imaging methods with activatable fluorescence probes, which show strong fluorescence only when the cells response to the target stimulants, are frequently used in modern biological experiments. However, the penetration of light is not so good for detecting cellular responses at deeper sites in living animals. This project aimed at the development of activatable bio-luminescence probes which show high bioluminescence only when the cells are active upon stimulation from outside. As a result, we succeeded in developing novel luciferin probes which emit quite long wavelength bioluminescence in near-infra red region, and activatable probes which emit strong fluorescence only when the cells response to some stimulants in vivo.
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Report
(4 results)
Research Products
(83 results)
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[Journal Article] Rapid Cancer Detection by Topically Spraying a Gamma-glutamyltranspeptidase-activated Fluorescent Probe2011
Author(s)
Urano Y, Sakabe M, Nobuyuki Kosaka, Ogawa M, Mitsunaga M, Asanuma D, Kamiya M, Young MR, Nagano T, Choyke PL, Kobayashi H
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Journal Title
Sci Transl Med
Volume: 3
Issue: 110
Pages: 110-119
DOI
Related Report
Peer Reviewed
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