| Budget Amount *help |
¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
Fiscal Year 2013: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2012: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
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| Outline of Final Research Achievements |
Live imaging methods with activatable fluorescence probes, which show strong fluorescence only when the cells response to the target stimulants, are frequently used in modern biological experiments. However, the penetration of light is not so good for detecting cellular responses at deeper sites in living animals. This project aimed at the development of activatable bio-luminescence probes which show high bioluminescence only when the cells are active upon stimulation from outside. As a result, we succeeded in developing novel luciferin probes which emit quite long wavelength bioluminescence in near-infra red region, and activatable probes which emit strong fluorescence only when the cells response to some stimulants in vivo.
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