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Cortical regeneration and nestin-expressing reactive astrocyte in a novel closed-head injury model "photo-injury mouse"

Research Project

Project/Area Number 23300133
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKobe University

Principal Investigator

MORITA Mitsuhiro  神戸大学, 理学(系)研究科(研究院), 准教授 (50297602)

Co-Investigator(Renkei-kenkyūsha) OKANO Hideyuki  慶應義塾大学, 医学部, 教授 (60160694)
MAEKAWA Shohei  神戸大学, 大学院理学研究科, 教授 (40173695)
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Keywords脳傷害 / 組織再生 / 活性化アストロサイト / 閉鎖性脳傷害 / ネスチン / 脳組織再生 / 創傷治癒 / アストロサイト / 神経幹細胞
Outline of Final Research Achievements

We have developed a novel brain injury model designated "photo-injury", which shows substantial cortical tissue regeneration. Further histological analysis showed a robust accumulation of nestin-expressing reactive astrocyte in the regenerating region. Since the genetic modification of the accumulation of the reactive astrocyte caused significant alteration of cortical regeneration, these cells were indicated to play a pivotal role in regeneration. Meanwhile, the majority of the reactive astrocyte was ablated during wound healing in our fate mapping study. These results suggest the nestin-expressing reactive astrocyte is one of the determinant of the process brain lesion and a potent diagnostic as well as therapeutic target of brain injury.

Report

(4 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (21 results)

All 2015 2014 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Characterization of neural stem cells and their progeny in the sensory circumventricular organs of adult mouse2015

    • Author(s)
      Furube, E. Morita, M. Miyata, S.
    • Journal Title

      Cell & Tissue Research

      Volume: 未定

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ganglioside contained in the NAP-22 fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin.2015

    • Author(s)
      Kobayashi, Y., Ronan da Silva, Kumanogoh, H., Miyata, S., Sato, C., Kitajima, K., Nakamura, S., Morita, M., Hayashi, F., Maekawa, S.
    • Journal Title

      J Neurosci Res

      Volume: 未定

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 新規脳傷害モデル「光傷害」における脳組織再生とグリア細胞の活性化2014

    • Author(s)
      森田光洋
    • Journal Title

      脳循環代謝

      Volume: 25 Pages: 63-66

    • NAID

      130004708202

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structures of septin filaments prepared from rat brain and expressed in bacteria.2013

    • Author(s)
      Maimaitiyiming, M., Kumanogoh, H., Nakamura, S., Morita, M., and Maekawa, S.
    • Journal Title

      Protein expression and purification

      Volume: 87 Issue: 2 Pages: 67-71

    • DOI

      10.1016/j.pep.2012.10.006

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of dynamin as a septin-binding protein.2013

    • Author(s)
      Maimaitiyiming, M., Kobayashi, Y., Kumanogoh, H., Nakamura, S., Morita, M., and Maekawa, S.
    • Journal Title

      Neurosci Lett

      Volume: 534 Pages: 322-6

    • DOI

      10.1016/j.neulet.2012.12.002

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of NAP-22 with brain glutamic acid decarboxylase (GAD)2013

    • Author(s)
      Maekawa, M., Kobayashi, Y., Odagaki, S., Makino, M., Kumanogoh, H., Nakamura, S., Morita, M., Hayashi, F.
    • Journal Title

      Neurosci Lett

      Volume: 537 Pages: 50-4

    • DOI

      10.1016/j.neulet.2013.01.030

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Astrocyte activation and wound healing in intact-skull mouse after focal brain injury.2012

    • Author(s)
      Suzuki T, Sakata H, Kato C, Connor JA and Morita M.
    • Journal Title

      Eur J Neurosci.

      Volume: 36 Issue: 12 Pages: 3653-3664

    • DOI

      10.1111/j.1460-9568.2012.08280.x

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effect of NAP-22 on the phosphatase activity of synaptojanin-12011

    • Author(s)
      Takaichi, R, et al
    • Journal Title

      J Neurosci Res

      Volume: 90 Issue: 1 Pages: 21-27

    • DOI

      10.1002/jnr.22740

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] STAT3 Signaling in Perilesional Nestin-Expressing Reactive Astrocyte is Required for Cortical Recovery after Closed-Head Injury.2014

    • Author(s)
      Morita, M. and Watanabe, A.
    • Organizer
      Society for Neurosci
    • Place of Presentation
      Washington DC
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2013 Annual Research Report
  • [Presentation] Two adenosine release mechanisms in rat hippocampus Neuroscience 20142014

    • Author(s)
      Fujii, Y. Yamashiro, K. and Morita, M.
    • Organizer
      日本神経科学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2013 Annual Research Report
  • [Presentation] STAT3 signaling in perilesional nestin-expressing reactive astrocyte is required for cortical recovery after closed-head injury.2014

    • Author(s)
      Watanabe, A and Morita, M.
    • Organizer
      日本神経科学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2013 Annual Research Report
  • [Presentation] The evoked increase of extracellular adenosine in rat hippocampus CA1 region depends on L-type Ca2+ channel.2013

    • Author(s)
      Morita, M. and Yamashiro, K.
    • Organizer
      Neuroscience 2013 (日本神経科学会)
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新規脳傷害モデル「光傷害」における脳組織再生とグリア細胞の活性化2013

    • Author(s)
      森田光洋
    • Organizer
      日本脳循環代謝学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Monitoring adenosine level in rat hippocampal slice by adenosine sensor cell2012

    • Author(s)
      Morita, M. and Yamashiro, K.
    • Organizer
      Neuroscience 2012 (日本神経科学会)
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] Adenosine release in rat hippocampal slice measured by a novel adenosine sensor cell2012

    • Author(s)
      Morita, M. and Yamashiro, K.
    • Organizer
      The 55th Annual Meeting for the Japanese Society for Neurochemistry
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] Monitoring adenosine level in rat hippocampal slice by adenosine sensor cell2012

    • Author(s)
      Morita, M. and Yamashiro, K.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Monitoring adenosine level in rat hippocampal slice by adenosine sensor cell2012

    • Author(s)
      Morita M, Yamashiro K
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      名古屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] Adenosine release in rat hippocampal slice measured by a novel adenosine sensor cell2012

    • Author(s)
      Morita M, Yamashiro K
    • Organizer
      The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry / The 55th Annual Meeting for the Japanese Society for Neurochemistry
    • Place of Presentation
      神戸
    • Related Report
      2011 Annual Research Report
  • [Presentation] Monitoring adenosine level in rat hippocampal slice by adenosine sensor cell2012

    • Author(s)
      Morita M, Yamashiro K
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      New Orleans
    • Related Report
      2011 Annual Research Report
  • [Presentation] Perilesional nestin-expressing reactive astrocytes and cortical tissue recovery in a novel closed-head injury model, photo injury2011

    • Author(s)
      Morita M
    • Organizer
      10th European Meeting on Glial Cells in Health and Disease
    • Place of Presentation
      Prague, Czech Republic
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] ネスチン陽性活性化アストロサイトの放射性イメージング剤2013

    • Inventor(s)
      森田光洋、岩井久美子
    • Industrial Property Rights Holder
      森田光洋、岩井久美子
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-227592
    • Filing Date
      2013-10-31
    • Related Report
      2012 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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