| Budget Amount *help |
¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
|
|---|
| Outline of Final Research Achievements |
DNA double-strand breaks (DSBs) are repaired by the error-free homologous recombination pathway and error-prone non-homologous end joining. Ubiquitination is indispensable post-translational modification for DSB response. Here, we focused on the mechanism of BRCA1-independent HR and ubiquitination-dependent regulation of HR. In this research project, we found that E3 ubiquitin ligase RNF8 regulates RAD51 assembly at DSB sites in the absence of BRCA1 and 53BP1. We also found that deubiquitinating enzyme OTUB2 finely regulates DSB-induced ubiquitination and enables HR.
|
