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Two-dimensional alignment technique of biomolecules using microfluidic format

Research Project

Project/Area Number 23310092
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Microdevices/Nanodevices
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

MIYAZAKI Masaya  独立行政法人産業技術総合研究所, 生産計測技術研究センター, 研究チーム長 (70344231)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Hiroshi  東海大学, 阿蘇教養教育センター, 准教授 (00466236)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywordsマイクロ化学システム / 表面 / 固定化 / 標識 / ナノマイクロ化学 / 酵素反応 / 規則配置 / マイクロリアクタ / 固定化技術
Research Abstract

In this study, we developed the multi-step enzymatic reaction technology using microchemical device to enable enzymatic reaction effectively to produce biologically active molecule. The two-dimensional alignment of molecule was enabled by using DNA as the templates. The template DNA molecules were immobilized within microchannel by using electorchemical methods or other techniques. Using this template, molecules can be immobilized and aligned on the DNA molecules. Furthermore, we developed microreactors for multi-step enzymatic reaction using these immobilized techniques. The microreactor showed possibility as an effective biologically active molecule processing device.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (44 results)

All 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 12 results) Presentation (21 results) (of which Invited: 6 results) Book (7 results) Remarks (1 results)

  • [Journal Article] Cationic Comb-Type Copolymer Excludes Intercalating Dye from DNA Without Inducing DNA Condensation2014

    • Author(s)
      Atsushi Maruyama, Naotaka Sonda, Kohei Yamasaki, Masanori Hirano, Satoru Kidoaki, Naohiko Shimada, Masatoshi Maeki, Masaya Miyazaki
    • Journal Title

      Current Nanoscience

      Volume: 10 Pages: 185-188

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Refolding Techniques for Biologically Active Recombinant Proteins from Inclusion Bodies2014

    • Author(s)
      Hiroshi Yamaguchi, Masaya Miyazaki
    • Journal Title

      Biomolecules

      Volume: 4 Issue: 1 Pages: 235-251

    • DOI

      10.3390/biom4010235

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlling Protein Crystal Nucleation by Droplet-Based Microfluidics2014

    • Author(s)
      Masatoshi Maeki, Yuki Teshima, Saori Yoshizuka, Hiroshi Yamaguchi, Kenichi Yamashita, Masaya Miyazaki
    • Journal Title

      CHEMISTRY-A EUROPEAN JOURNAL

      Volume: 20 Issue: 4 Pages: 1049-1056

    • DOI

      10.1002/chem.201303270

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simple density-based particle separation in a microfluidic chip2014

    • Author(s)
      Daisuke Sugiyama, Yuki Teshima, Kenichi Yamanaka, Maria Portia Briones, Masatoshi Maeki, Kenichi Yamashita, Masashi Takahashi, Masaya Miyazaki
    • Journal Title

      ANALYTICAL METHODS

      Volume: 6 Issue: 1 Pages: 308-311

    • DOI

      10.1039/c3ay40971f

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzyme-immobilized reactors for rapid and efficient sample preparation in MS-based proteomic studies2013

    • Author(s)
      Hiroshi Yamaguchi, Masaya Miyazaki
    • Journal Title

      Proteomics

      Volume: 13 Pages: 457-466

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A method for generating metastable crystal in a microdroplet2013

    • Author(s)
      Yuki Teshima, Masatoshi Maeki, Kenichi Yamashita, Masaya Miyazaki
    • Journal Title

      CRYSTENGCOMM

      Volume: 15 Issue: 46 Pages: 9874-9877

    • DOI

      10.1039/c3ce40940f

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlling One Protein Crystal Growth by Droplet-based Microfluidics System2013

    • Author(s)
      Hiroshi Yamaguchi, Masatoshi Maeki, Kenichi Yamashita, Hiroyuki Nakamura, Masaya Miyazaki, Hideaki Maeda
    • Journal Title

      The Journal of Biochemistry

      Volume: 153 Issue: 4 Pages: 339-346

    • DOI

      10.1093/jb/mvt001

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improving the catalytic performance of laccase using a novel continuous-flow microreactor2013

    • Author(s)
      L. Lloret, G. Eibes, M.T. Moreira, G. Feijoo, J.M. Lema, M. Miyazaki
    • Journal Title

      Chemical Engineering Journal

      Volume: 223 Pages: 497-506

    • DOI

      10.1016/j.cej.2013.03.018

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マイクロ化学システムを用いるプロセッシング技術の開発2012

    • Author(s)
      宮崎真佐也
    • Journal Title

      化学とマイクロ・ナノシステム

      Volume: 11(2) Pages: 16-17

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 「微小流体光学(Optofl uidics):マイクロフォトニクスと流体の融合」 解説小特集号によせて2012

    • Author(s)
      興雄司、宮崎真佐也
    • Journal Title

      レーザー研究

      Volume: 40 Pages: 911-912

    • NAID

      130007891231

    • Related Report
      2012 Annual Research Report
  • [Journal Article] マイクロ流体チップへのオプティクス埋め込み2012

    • Author(s)
      山下健一、宮崎真佐也
    • Journal Title

      レーザー研究

      Volume: 40 Pages: 942-946

    • NAID

      10031140360

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PROTEASE-IMMOBILIZED MICROREACTOR FOR RAPID AND SITE-SPECIFIC AFFINITY TAG CLEAVAGE2012

    • Author(s)
      Asanomi, Y., et al
    • Journal Title

      PEPTIDE SCIENCE 2011

      Pages: 395-396

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzyme-Immobilized MicrofluidicProcess Reactors2011

    • Author(s)
      Yuya Asanomi, Hiroshi Yamaguchi, Masaya Miyazaki, Hideaki Maeda
    • Journal Title

      Molecules

      Volume: 16(7) Issue: 7 Pages: 6041-6059

    • DOI

      10.3390/molecules16076041

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Poly-lysine supported cross-linked enzyme aggregates with efficient enzymatic activity and high operational stability2011

    • Author(s)
      Yamaguchi, H., et al
    • Journal Title

      Catalysis Science & Technology

      Volume: 1 Issue: 7 Pages: 1259-1261

    • DOI

      10.1039/c1cy00084e

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] タンパク質・ペプチドの配列解析用ツールとしてのマイクロ化学デバイス2011

    • Author(s)
      宮崎真佐也
    • Journal Title

      Peptide Newsletter Japan

      Pages: 8-11

    • Related Report
      2011 Annual Research Report
  • [Presentation] Microfluidic devices for protein sequence and structure analysis2014

    • Author(s)
      Masaya Miyazaki
    • Organizer
      Pittcon 2014
    • Place of Presentation
      McCormick Hall, Cigago, IL, USA
    • Year and Date
      2014-03-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Microfluidic devices for protein sequence and structure analysis2014

    • Author(s)
      Masaya Miyazaki
    • Organizer
      Pittcon 2014
    • Place of Presentation
      McCormick Hall (Cicago, IL, USA)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] An approach for controlling polymorphism of organic crystals by using droplet-based microfluidics2013

    • Author(s)
      Masatoshi Maeki, Yuki Teshima, Kenichi Yamashita, Masaya Miyazaki
    • Organizer
      2013 Joint Japan/Taiwan/Korea Chemical Engineering Conference
    • Place of Presentation
      崇城大学熊本市民ホール(熊本市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マイクロ空間を用いる有機分子の晶析制御技術2013

    • Author(s)
      宮崎 真佐也
    • Organizer
      2013化学工学会九州セミナー
    • Place of Presentation
      崇城大学熊本市民ホール(熊本市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] マイクロ流体デバイスを用いるタンパク質のプロセッシング2013

    • Author(s)
      宮崎真佐也
    • Organizer
      第37回 蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      新湯ホテル(雲仙市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] マイクロ流体デバイスとミクロシーディング法による好冷細菌Pseudoalteromonas sp.AS-131由来グルコキナーゼの結晶化の制御2013

    • Author(s)
      真栄城正寿,湧川盛洋,Ashtamurthy Pawate,河本正秀,山下 健一,Paul J. A. Kenis,渡邉啓一,宮崎真佐也
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Crystallization of Glucokinase from Psychrophilic Pseudoalteromonas sp. AS-131 in Microfluidic Chip and Its Application for On-chip X-ray Diffraction2013

    • Author(s)
      Masaya Miyazaki, Masatoshi Maeki, Ashtamurthy Pawate, Wakukawa Morihiro, Kenichi Yamashita, Keiichi Watanabe, Paul J. A. Kenis
    • Organizer
      RSC Tokyo International Conference 2013
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 微小液滴を用いた有機結晶の多型制御2013

    • Author(s)
      手島裕貴,真栄城正寿,山下健一,宮崎真佐也
    • Organizer
      化学とマイクロ・ナノシステム学会第27回研究会
    • Place of Presentation
      東北大学(仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マイクロ流体デバイスを用いたタンパク質結晶のシーディング2013

    • Author(s)
      真栄城正寿,湧川盛洋,Ashtamurthy Pawate,山下健一,渡邉啓一,Paul J. A. Kenis,宮崎真佐也
    • Organizer
      化学とマイクロ・ナノシステム学会第27回研究会
    • Place of Presentation
      東北大学(仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マイクロ流体デバイスを用いた好冷細菌Pseudoalteromonas sp.AS-131由来グルコキナーゼ結晶のシーディング2013

    • Author(s)
      真栄城正寿,湧川盛洋,Ashtamurthy Pawate,山下健一,Paul J. A. Kenis,宮崎真佐也,渡邉啓一
    • Organizer
      平成25年度日本生化学会九州支部例会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マイクロ化学システムを用いるプロセッシング技術の開発2012

    • Author(s)
      宮崎真佐也
    • Organizer
      第25回化学とマイクロナノシステム研究会
    • Place of Presentation
      熊本県崇城大学
    • Year and Date
      2012-05-17
    • Related Report
      2013 Final Research Report
  • [Presentation] ミクロ流路を利用したDNA・リガンド相互作用の一分自計測とその応用2012

    • Author(s)
      宮崎真佐也
    • Organizer
      「物質・デバイス領域共同研究拠点特定課題」B-5課題研究集会
    • Place of Presentation
      福岡県、九州大学(招待講演)
    • Year and Date
      2012-03-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] マイクロ流体デバイスを用いる生体関連物質プロセッシング技術の開発2011

    • Author(s)
      宮崎真佐也
    • Organizer
      仙台マイクロナノ国際フォーラム
    • Place of Presentation
      宮城県江陽グランドホテル
    • Year and Date
      2011-11-08
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロ流体デバイスを用いる生体関連物質プロセッシング技術の開発2011

    • Author(s)
      宮崎真佐也
    • Organizer
      仙台マイクロナノ国際フォーラム2011
    • Place of Presentation
      宮城県、江陽グランドホテル(招待講演)
    • Year and Date
      2011-11-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] PROTEASE-IMMOBILIZED MICROREACTOR FOR RAPID AND SITE-SPECIFIC AFFINITY TAG CLEAVAGE2011

    • Author(s)
      宮崎真佐也
    • Organizer
      第48回ペプチド討論会
    • Place of Presentation
      北海道、札幌国際コンベンションセンター
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] フローストレッチング法を利用したポリカチオン-DNA相互作用の解析2011

    • Author(s)
      尊田尚孝, ほか8名
    • Organizer
      第21回バイオ・高分子シンポジウム
    • Place of Presentation
      大阪府、関西大学
    • Year and Date
      2011-07-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] マイクロ化学システムを用いるプロセッシング技術の開発

    • Author(s)
      宮崎真佐也
    • Organizer
      第25回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      崇城大学、熊本市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] マイクロ化学デバイスを用いた効率的アフィニティータグ切断法の開発

    • Author(s)
      浅海裕也、古賀裕也、山口浩、渡口啓史、関 清彦、光富勝、兒玉浩明、前田英明、宮崎真佐也
    • Organizer
      平成24年度生化学会九州支部例会
    • Place of Presentation
      福岡大学、福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Laccase-immobilized microreactors for the removal of estrogenic compounds

    • Author(s)
      Lucia Lloret Caulonga、宮崎 真佐也、G. Eibes、 G. Feijoo、 M.T. Moreira、J.M. Lema
    • Organizer
      第25回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      崇城大学、熊本市
    • Related Report
      2012 Annual Research Report
  • [Presentation] マイクロ化学システムを用いるプロセッシング技術

    • Author(s)
      宮崎真佐也
    • Organizer
      第3回生物計測化学懇談会
    • Place of Presentation
      北海道大学、札幌市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ミクロ流路を利用したDNA・リガンド相互作用の一分子計測とその応用

    • Author(s)
      宮崎真佐也
    • Organizer
      平成24年度「物質・デバイス領域共同研究拠点特定課題」B-5課題研究集会
    • Place of Presentation
      九州大学、福岡市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] Microreactors in Organic Synthesis and Catalysis, 2nd ed. (Wirth, T.ed.)2013

    • Author(s)
      Masaya Miyazaki, Maria Portia Briones-Nagata, Takeshi Honda, Hiroshi Yamaguchi
    • Publisher
      Wiley-VCH
    • Related Report
      2013 Final Research Report
  • [Book] Microreactors in Organic Synthesis and Catalysis, Second Edition2013

    • Author(s)
      Masaya Miyazaki, Maria Portia Briones Nagata, Takeshi Honda, Hiroshi Yamaguchi
    • Total Pages
      77
    • Publisher
      Wiley-VCH
    • Related Report
      2013 Annual Research Report
  • [Book] Microreactors in Organic Chemistry and Catalysis (Thomas Wirth ed.), 2nd ed.2013

    • Author(s)
      Masaya Miyazaki et al.
    • Total Pages
      465
    • Publisher
      Wiley-VCH
    • Related Report
      2012 Annual Research Report
  • [Book] Integrative Proteomics (Leung E. ed)2012

    • Author(s)
      Hiroshi Yamaguchi, Masaya Miyazaki, Hideaki Maeda
    • Publisher
      InTech-Croatia
    • Related Report
      2013 Final Research Report
  • [Book] Integrative Proteomics2012

    • Author(s)
      Yamaguchi, H., et al
    • Total Pages
      442
    • Publisher
      InTech-Croatia
    • Related Report
      2011 Annual Research Report
  • [Book] Methods in Molecular Biology Vol. 815 Functional Genomics, Second Edition (Kaufmann, M. & Klinger, C. eds)2011

    • Author(s)
      Hiroshi Yamaguchi, Masaya Miyazaki, Hideaki Maeda
    • Publisher
      Humana Press
    • Related Report
      2013 Final Research Report
  • [Book] Methods in Molecular Biology Series-Functional Genomics, Second Edition2011

    • Author(s)
      Yamaguchi, H., et al
    • Total Pages
      418
    • Publisher
      Springer (Humana Press)
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      https://staff.aist.go.jp/m.miyazaki/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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