Development of microchip for rapid detection of adipokines and early diagnosis of diabetes mellitus.
| Project/Area Number |
23310093
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KATAOKA Masatoshi 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (20224438)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | マイクロチップ基板 / サンドイッチELISA / 微細化インクジェット / IL-6 / TNF-α / アディポネクチン / 糖尿病 / マイクロチップ / アディポカイン / TNF-alpha |
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| Research Abstract |
Interleukin-6 and TNF-a in the blood are frequently measured by conventional sandwich ELISA using 96 wells plate. However, the conventional assay is time-consuming, and is not suitable for rapid diagnosis. To overcome these drawbacks, we performed a sandwich ELISA on a microchip for simultaneous analysis. The microchip was made of cyclic olefin copolymer with 4 straight microchannels that were 300 um wide and 100 um deep. For the construction of the sandwich ELISA for IL-6 or TNF-a, we used a piezoelectric inkjet printing system for the deposition and fixation of the 1st antibody on the surface of the each microchannel. This microchip-based assay enabled us to determine simultaneously blood IL-6 and TNF-a with accuracy, satisfactory sensitivity, time saving ability, and low consumption of sample and reagents, and thus will be applicable to early diagnosis of diabetes mellitus.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip2013
Author(s)
Abe K, Hashimoto Y, Yatsushiro S, Yamamura S, Bando M, Hiroshima Y, Kido J, Tanaka M, Shinohara Y, Ooie T, Baba Y, Kataoka M
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Journal Title
PLoS One
Volume: 8
Issue: 1
Pages: e53620-e53620
DOI
Related Report
Peer Reviewed
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[Journal Article] Singlet oxygen induced products of linoleates, 10-and 12(Z,E)-hydroxyoctadecadienoic acid (HODE), can be potential biomarkers for early detection of type 2 diabetes2013
Author(s)
Umeno A, Shichiri M, Ishida N, Hashimoto Y, Abe K, Kataoka M, Yoshino K, Hagihara Y, Aki N, Funaki M, Asada Y, Yoshida Y
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Journal Title
PLoS One
Volume: 8
Issue: 5
Pages: e63542-e63542
DOI
Related Report
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