| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Research Abstract |
Interleukin-6 and TNF-a in the blood are frequently measured by conventional sandwich ELISA using 96 wells plate. However, the conventional assay is time-consuming, and is not suitable for rapid diagnosis. To overcome these drawbacks, we performed a sandwich ELISA on a microchip for simultaneous analysis. The microchip was made of cyclic olefin copolymer with 4 straight microchannels that were 300 um wide and 100 um deep. For the construction of the sandwich ELISA for IL-6 or TNF-a, we used a piezoelectric inkjet printing system for the deposition and fixation of the 1st antibody on the surface of the each microchannel. This microchip-based assay enabled us to determine simultaneously blood IL-6 and TNF-a with accuracy, satisfactory sensitivity, time saving ability, and low consumption of sample and reagents, and thus will be applicable to early diagnosis of diabetes mellitus.
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