Photo-physiological acclimation of cyanobacteria diversified in various marine environments

• Project/Area Number: 23370013
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: Kobe University
• Principal Investigator: Murakami Akio 神戸大学, 内海域環境教育研究センター, 准教授 (50304134)
• Co-Investigator(Kenkyū-buntansha): HIROSE Euichi 琉球大学, 理学部, 教授 (30241772) ; AKIMOTO Seiji 神戸大学, 分子フォトサイエンス研究センター, 准教授 (40250477)
• Research Collaborator: YOKONO Makio 北海道大学, 低温科学研究所 ; TAKAICHI Shinichi 日本医科大学 ; YAMANAKA Rio 姫路獨協大学, 薬学部 ; UCHIDA Hiroko 神戸大学, 内海域環境教育研究センター ; HAMDA Fumiya 神戸大学, 大学院理学研究科
• Project Period (FY): 2011-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: シアノバクテリア / 光合成色素 / 光環境 / 海洋 / 光合成 / 海洋生態

Outline of Final Research Achievements

Cyanobacteria are the pioneer microorganisms that joined the water-splitting and oxygen-evolving reaction to the solar-light-energy conversion and photosynthetic organic carbon producing system. In ocean covering 70% of Earth’s surface, where the evolution and diversification of life continues over several billion years, various and colorful cyanobacteria grow. In this study, we revealed how cyanobacteria operate photosynthetic system and how cyanobacteria adapt to various environmental factors in the euphotic zone of ocean.

Research Products

• [Journal Article] Excitation relaxation dynamics and energy transfer in pigment-protein complexes of a dinoflagellate revealed by ultrafast fluorescence spectroscopy (2016)
  • Author(s): Tanaka K, Iida S, Takaichi S, Mimuro M, Murakami A and Akimoto S
  • Journal Title: Photosynthesis Research Volume: 128 Issue: 1-3 Pages: 9-9
  • DOI: 10.1007/s11120-016-0238-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Carotenogenesis diversification in phylogenetic lineages of Rhodophyta (2016)
  • Author(s): Takaichi S, Yokoyama A, Mochimaru M, Uchida H and Murakami A
  • Journal Title: Journal of Phycology Volume: 52 Issue: 3 Pages: 10-10
  • DOI: 10.1111/jpy.12411
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comparative analysis of ultrafast excitation energy-transfer pathways in three strains of divinyl chlorophyll a/b-containing cyanobacterium, Prochlorococcus marinus (2015)
  • Author(s): Hamada F, Murakam A and Akimoto S
  • Journal Title: Journal of Physical Chemistry B Volume: 119 Issue: 51 Pages: 15593-15600
  • DOI: 10.1021/acs.jpcb.5b10073
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Diplosoma gumavirens (Ascidiacea, Didemnidae): The first record of photosymbiotic ascidians in the Taiwan Strait (2015)
  • Author(s): Hirose E, Hirose M and Shy J-Y
  • Journal Title: Marine Biodiversity Records Volume: 8 Pages: 6-6
  • DOI: 10.1017/s1755267215000330
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Selective synthesis of cinnamyl alcohol by cyanobacterial photobiocatalysts. (2015)
  • Author(s): Rio Yamanaka, Kaoru Nakamura, Masahiko Murakami, Akio Murakami
  • Journal Title: Tetrahedron Letters, Volume: 56 Issue: 9 Pages: 1089-1091
  • DOI: 10.1016/j.tetlet.2015.01.092
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functional characterization of the FNT family nitrite transporter of marine picocyanobacteria. (2015)
  • Author(s): Shin-ichi Maeda, Akio Murakami, Hisashi Ito, Ayumi Tanaka, Tatsuo Omata
  • Journal Title: Life Volume: 5 Issue: 1 Pages: 432-446
  • DOI: 10.3390/life5010432
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Aquatic plant surface as a niche for methanotrophs. (2014)
  • Author(s): Yoshida N, Iguchi H, Yurimoto H, Murakami A, Sakai Y
  • Journal Title: Frontiers in Microbiology / Terrestrial Microbiology Volume: 5 Pages: 1-8
  • DOI: 10.3389/fmicb.2014.00030
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Possible involvement of a tetrahydrobiopterin in photoreception for UV-B-induced anthocyanin synthesis in carrot. (2014)
  • Author(s): Takeda J., Nakata R., Ueno H., Murakami A., Iseki M. and Watanabe M.
  • Journal Title: Photochemistry and Photobiology Volume: 90 Issue: 5 Pages: 1043-1049
  • DOI: 10.1111/php.12302
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] All of α-carotene and its derivatives have a sole chirality? (2013)
  • Author(s): Takaichi S, Murakami A, Mochimaru M.
  • Journal Title: “Photosynthesis Research for Food, Fuel and Future: 15th International Conference on Photosynthesis. Volume: 1 Pages: 135-138
  • Peer Reviewed
• [Journal Article] Opposite chilarity of α-carotene in unusual cyanobacteria with unique chlorophylls, Acaryochloris and Prochlorococcus (2012)
  • Author(s): Takaichi, S.
  • Journal Title: Plant Cell Physiol. Volume: 53 Issue: 11 Pages: 1881-1888
  • DOI: 10.1093/pcp/pcs126
  • Peer Reviewed
• [Journal Article] Excitation energy relaxation in a symbiotic cyanobacterium, Prochloron didemni, occurring in coral-reef ascidians, and in a free-living cyanobacterium,Prochlorothrix hollandica. (2012)
  • Author(s): Hamada, F., Yokono, M., Hirose, E., Murakami, A., Akimoto, S
  • Journal Title: Biochim. Biophys. Acta Volume: 1817 Issue: 11 Pages: 1992-1997
  • DOI: 10.1016/j.bbabio.2012.06.008
  • Peer Reviewed
• [Journal Article] α-Carotene and its derivatives have a sole chirality in phototrophic organisms? (2012)
  • Author(s): Takaichi S, Murakami A, Mochimaru M and Yokoyama A.
  • Journal Title: Acta Biochimica Polonica Volume: 59 Pages: 159-161
  • Peer Reviewed
• [Journal Article] Stabilization and modulation of the phycobilisome by calcium in the calciphilic freshwater red alga Bangia atropurpurea (2012)
  • Author(s): Makio Yokono, et al
  • Journal Title: Biochimica et Biophysica Acta (BBA)-Bioenergetics Volume: 1817 Issue: 2 Pages: 306-311
  • DOI: 10.1016/j.bbabio.2011.11.002
  • Peer Reviewed
• [Journal Article] Microscopic anatomy and pigment characterization of coral-encrusting black sponge with cyanobacterial symbiont, Terpios hoshinota. (2011)
  • Author(s): Euichi Hirose, Akio Murakami
  • Journal Title: Zoological Science Volume: 28 Issue: 3 Pages: 199-205
  • DOI: 10.2108/zsj.28.199
  • NAID: 40018724185
  • Peer Reviewed
• [Journal Article] Molecular environments of divinyl chlorophylls in Prochlorococcusand Synechocystis: Differences in fluorescence properties with chlorophyll replacement (2011)
  • Author(s): Mimuro, M., Murakami, A., Tomo, T., Tsuchiya, T., Watabe, K., Yokono, M., Akimoto, S
  • Journal Title: Biochim. Biophys. Acta Volume: 1807 Issue: 5 Pages: 471-481
  • DOI: 10.1016/j.bbabio.2011.02.011
  • Peer Reviewed
• [Journal Article] Reduction of exogenous ketones depends upon NADPH generated photosynthetically in cells of the cyanobacterium Synechococcus PCC 7942. (2011)
  • Author(s): Rio Yamanaka, Kaoru Nakamura, Akio Murakami
  • Journal Title: AMB Express Volume: 1:24 Issue: 1 Pages: 8-8
  • DOI: 10.1186/2191-0855-1-24
  • Peer Reviewed
• [Journal Article] Excitation energy transfer between photosystem II and photosystem I in red algae: Larger amounts of phycobilisome enhance spillover. (2011)
  • Author(s): Makio Yokono, Akio Murakami, Akio Murakami,
  • Journal Title: Biochimica et Biophysica Acta Volume: 1807 Issue: 7 Pages: 847-853
  • DOI: 10.1016/j.bbabio.2011.03.014
  • Peer Reviewed
• [Presentation] ケトカロテノイドを有する色素タンパク質複合体における超高速エネルギー移動 (2016)
  • Author(s): 秋本誠志・三室守・村上明男
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス（岩手県・盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Ultrafast excitation energy transfer pathways in divinyl chlorophyll a/b-containing cyanobacterium, Prochlorococcus marinus (2016)
  • Author(s): Fumiya Hamada, Akio Murakami, Seiji Akimoto
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス（岩手県・盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] 新しいタイプの脂質分子種を合成するシアノバクテリア (2015)
  • Author(s): 和田元・齊藤勝和・遠藤嘉一郎・小林康一・渡辺麻衣・池内昌彦・村上明男・村田紀夫
  • Organizer: 藍藻の分子生物学2015
  • Place of Presentation: かずさアカデミアホール（千葉県・千葉市）
  • Year and Date: 2015-11-16
• [Presentation] 藍藻イソプロパノール耐性株の光合成特性 (2015)
  • Author(s): 渡部和幸・金本優杞・新井小百合・清水一憲・本多裕之・広川安孝・花井泰三・村上明男
  • Organizer: 第67回日本生物工学会大会
  • Place of Presentation: 城山観光ホテル（鹿児島県・鹿児島市）
  • Year and Date: 2015-10-26
• [Presentation] Excitation relaxation dynamics and energy transfer in pigment-protein complexes containing keto-carotenoids (2015)
  • Author(s): Seiji Akimoto, Mamoru Mimuro, Akio Murakami
  • Organizer: 6th International Conference “Photosynthesis Research for Sustainability – 2015
  • Place of Presentation: Orthodox Academy of Grete Conference Center (Crete, Greece)
  • Year and Date: 2015-09-21
  • Int'l Joint Research
• [Presentation] ニチニチソウ組織におけるTerpenoid indole alkaloidの合成と蓄積機構の解明 (2015)
  • Author(s): 山本浩太郎・大西美輪・姉川彩・高橋勝利・水野初・村上明男・石崎公庸・山崎真巳・深城英弘・升島努・三村徹郎
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ:新潟コンベンションセンター (新潟県・新潟市）
  • Year and Date: 2015-09-06
• [Presentation] 近紫外線(ultrviolet-A)照射によるシアノバクテリア増殖抑制効果の解明 (2015)
  • Author(s): 西坂理沙・馬渡一諭・常冨愛香里・後藤茉凛・上番増明子・下畑隆明・上番増喬・榎本崇宏・芥川正武・木内陽介・金本優杞・村上明男・髙橋章
  • Organizer: 第251回 徳島医学会学術集会,
  • Place of Presentation: 徳島大学（徳島県・徳島市）
  • Year and Date: 2015-08-02
• [Presentation] Opposite chilarity of a-carotene in unusual cyanobacteria with unique chlorophylls, Acaryochloris and Prochlorococcus (2015)
  • Author(s): Shinichi Takaichi, Mari Mochimaru, Hiroko Uchida, Akio Murakami, Euichi Hirose
  • Organizer: 15th International Symposium on Phototrophic Prokaryotes: ISPP2015
  • Place of Presentation: Eberhard-Karis Universitaet (Tubingen, Germany)
  • Year and Date: 2015-08-02
  • Int'l Joint Research
• [Presentation] 超高速時間分解蛍光分光法によるシアノバクテリアProchlorococcusにおける光捕集機能の解明 (2015)
  • Author(s): 浜田文哉・村上明男・秋本誠志
  • Organizer: 第23回「光合成セミナー2015：反応中心と色素系の多様性」
  • Place of Presentation: 龍谷大学大宮キャンパス (京都府・京都市）
  • Year and Date: 2015-07-11
• [Presentation] Interspecies distribution of PSI-PSII megacomplex may relate to quenching ability of PSI (2015)
  • Author(s): Makio Yokono, Atsushi Takabayashi, Ayumi Tanaka, Akio Murakami, Seiji Akimoto
  • Organizer: Photosynthesis (GRS) Gordon Research Seminar “Beyond Steady-State Photosynthesis: Emerging Model Organisms and Technologies”
  • Place of Presentation: Bentley University, (Waltham, USA)
  • Year and Date: 2015-06-27
  • Int'l Joint Research
• [Presentation] 紅色植物門の系統分類とカロテノイド生合成経路の多様性 (2015)
  • Author(s): 高市真一・横山亜紀子・内田博子・村上明男・持丸真里
  • Organizer: 第56回日本植物学生理学会
  • Place of Presentation: 東京農大、世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 紅色植物門の系統分類とカロテノイド生合成経路の多様性 (2014)
  • Author(s): 高市真一・横山亜紀子・内田博子・村上明男・持丸真里
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学・生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Highly selective synthesis of cinnamyl alcohol by cyanobacteria as photobioctalysts (2014)
  • Author(s): Rio Yamanaka, Kaoru Nakayama, Masahiko Murakami and Akio Murakami
  • Organizer: Biocatalysis, Gordon Research Conference, Changing Paradigms in Catalysis,
  • Place of Presentation: Bryant University, Smithfield, RI, USA
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] Mosaic distribution of the genes encoding tetrapyrole biosynthesizing enzymes in bacteria (2014)
  • Author(s): Ryota kohata, Yuuki Akiyama, Akio Murakami, Yuichi Fujita, Hisashi Ito, Ayumi Tanaka and Ryoichi Tanaka
  • Organizer: International Conference on Porphyrins and Phthalocyanines (ICPP 8)
  • Place of Presentation: Turkey, Istanbul
  • Year and Date: 2014-06-22 – 2014-06-27
• [Presentation] ラン藻におけるProtoporphyrinogen IX oxidase遺伝子の多様性 (2014)
  • Author(s): 木幡亮哉・村上明男・藤田祐一・伊藤寿・田中歩・田中亮一
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 2014-03-18 – 2014-03-20
• [Presentation] Excitation energy transfer in cyanobacteria containing phycoerythrin (2013)
  • Author(s): Yuki Koge, Akio Murakami, Seiji Akimoto
  • Organizer: 第51回日本生物物理学会年会.
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2013-09-25 – 2013-09-27
• [Presentation] 紅藻の系統分類とカロテノイド合成系 (2013)
  • Author(s): 高市真一・横山亜紀子・村上明男
  • Organizer: 第27回カロテノイド研究談話会
  • Place of Presentation: 三重大学
  • Year and Date: 2013-09-19 – 2013-09-20
• [Presentation] バラの花色決定における液胞機能の解析 (2013)
  • Author(s): 西本朱里・大西美輪・姉川彩・高橋勝利・村上明男・七條千津子・石崎公庸・深城英弘・三村徹郎
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2013-09-13 – 2013-09-15
• [Presentation] Carotenoid composition including a-carotene of unusual cyanobacteria with unique chlorophylls: Acaryochloris (Chl a/d), Prochlorococcus (DV-Chl a/b), Prochlorothrix (Chl a/b) and Prochloron (Chl a/b) (2013)
  • Author(s): Shinichi Takaichi, Hiroko Uchida, Euichi Hirose, Mari Mochimaru, Akio Murakami.
  • Organizer: The 16th International Congress on Photosynthesis Research
  • Place of Presentation: St. Louis, MO, USA
  • Year and Date: 2013-08-11 – 2013-08-16
• [Presentation] フィコビリンをもつ真核藻類のカロテノイド組成と生合成：Carotenoids and their synthesis in algae containing phycobilins (2013)
  • Author(s): 高市真一・横山亜紀子・持丸真里・内田博子・村上明男
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] 海洋性ラン藻の亜硝酸イオン輸送体の機能解析 (2013)
  • Author(s): 前田真一・村上明男・伊藤寿・田中歩・小俣達男
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] α-Carotene found only in unusual chlorophyll-containing cyanobacteria, Acaryochloris and Prochlorococcus, among Prokaryotes (2012)
  • Author(s): Takaichi S, Uchida H, Murakami A, Hirose E, Mochimaru M, Tsuchiya T and Mimuro M
  • Organizer: 14th International Symposium on Phototrophic Prokaryotes
  • Place of Presentation: Porto, Portugal
• [Presentation] 特異なクロロフィルをもつシアノバクテリアにおけるカロテノイド (2012)
  • Author(s): 高市真一・内田博子・村上明男・広瀬裕一・土屋徹・持丸真里・横山亜紀子
  • Organizer: 第26 回カロテノイド研究談話会
  • Place of Presentation: 函館国際ホテル
• [Presentation] 特殊なクロロフィルを持つシアノバクテリアのカロテノイド (2012)
  • Author(s): 高市真一・内田博子・村上明男・広瀬裕一・土屋徹・持丸真里・横山亜紀子
  • Organizer: 日本植物学会大会
  • Place of Presentation: 兵庫県立大学姫路
• [Presentation] 光合成色素が主導する光誘起電子授受反応 (2012)
  • Author(s): 村上明男
  • Organizer: 第64回日本生物工学会大会 シンポジウム“日本から発信する、エネルギー革新省エネ型炭素固定とe-バイオの融合”
  • Place of Presentation: 神戸国際会議場
  • Invited
• [Presentation] Characterization of diatom silica frustules by IR micro-spectroscopy (1): polymerization degree of Si-O. (2012)
  • Author(s): Leila Alipour, Satoru Nakashima, Osamu Oku, Akio Murakami and Rika Harui
  • Organizer: 平成24年度日本分光学会年次講演会・国際シンポ「バイオセンシングと触媒反応解析への分光学の応用」
  • Place of Presentation: 東工大百年記念館
• [Presentation] シアノバクテリアにおけるメラトニンの同定 (2012)
  • Author(s): 赤塚涼佑・高根正之・海老原充・村上明男・井上和仁・関口俊男・鈴木信雄・服部淳彦
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学
• [Presentation] ラン藻－光合成システムとその機能，調節，多様性 (2012)
  • Author(s): 村上明男
  • Organizer: 第4回 高資源循環ポリマーセンターシンポジウム（第2回サクラン研究会）
  • Place of Presentation: JAIST (品川キャンパス)
  • Invited
• [Presentation] シアノバクテリアのα-カロテン：分布と立体異性α-Carotene from cyanobacteria: distribution and stereochemistry (2012)
  • Author(s): 高市真一・内田博子・村上明男・広瀬裕一・持丸真里・土屋徹・三室守
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] シアノバクテリアProchloronにおける励起エネルギー移動 (2011)
  • Author(s): 浜田文哉・横野牧生・広瀬裕一・村上明男・秋本誠志
  • Organizer: 第19回光合成の色素系と反応中心に関するセミナー
  • Place of Presentation: 大阪大学
• [Presentation] シアノバクテリアProchlorococcusにおけるジビニルクロロフィルの励起緩和ダイナミクス (2011)
  • Author(s): 秋本誠志・横野牧生・村上明男・鞆達也・土屋徹・渡部和幸・三室守
  • Organizer: 第19回光合成の色素系と反応中心に関するセミナー
  • Place of Presentation: 大阪大学
• [Presentation] クロロフィルbをもつラン藻ProchloronとProchlorococcusの扱い (2011)
  • Author(s): 内田博子・広瀬裕一・三室守・村上明男
  • Organizer: 第19回光合成の色素系と反応中心に関するセミナー
  • Place of Presentation: 大阪大学
• [Presentation] 酸素発生光合成生物のα-カロテン：Acaryochlorisの例外的な立体構造 (2011)
  • Author(s): 高市真一・持丸真理・土屋徹・内田博子・村上明男・三室守
  • Organizer: 第19回光合成の色素系と反応中心に関するセミナー
  • Place of Presentation: 大阪大学
• [Presentation] a-Carotene and its derivatives have a sole chirality in phototrophic organisms? (2011)
  • Author(s): Shinichi Takaichi, Akio Murakam, Mari Mochimaru
  • Organizer: 16th International Symposium on carotenoids
  • Place of Presentation: Krakow, Poland
• [Presentation] シアノバクテリアAcaryochlorisのα-カロテン：例外的な立体異性 (2011)
  • Author(s): 高市真一・持丸真里・土屋徹・内田博子・村上明男・三室守
  • Organizer: 第25 回カロテノイド研究談話会
  • Place of Presentation: つくば国際会議
• [Presentation] 海洋ラン藻におけるクロロフィルb：ProchlorococcusとProchloron (2011)
  • Author(s): 村上明男・内田博子・広瀬裕一・浜田文哉・秋本誠志
  • Organizer: 植物学会第75回大会
  • Place of Presentation: 東京大学
• [Presentation] 種々のホヤ中における藍藻Prochloronの励起緩和ダイナミクス (2011)
  • Author(s): 浜田文哉・横野牧生・広瀬裕一・村上明男・秋本誠志
  • Organizer: 第５回分子科学討論会
  • Place of Presentation: 札幌コンベンションセンター
• [Book] 「光と生命の事典」（日本光生物学協会 光と生命の事典 編集委員会 編） (2016)
  • Author(s): 村上明男（項目分担執筆：補色馴化、口絵写真）
  • Total Pages: 436
  • Publisher: 朝倉書店
• [Book] 「植物学の百科事典」（日本植物学会編 (2016)
  • Author(s): 村上明男（項目分担執筆：藍藻）
  • Total Pages: 800
  • Publisher: 丸善出版
• [Book] 藻類ハンドブック(監修、渡辺信) (2012)
  • Author(s): 村上明男
  • Publisher: エヌ・ティー・エス