Slow axonal transport driven by directional actin turnover
| Project/Area Number |
23370088
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
INAGAKI Naoyuki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (20223216)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIURA Tadao 奈良先端科学技術大学院大学, 情報科学研究科, 准教授 (60304010)
SAKUMURA Yuichi 愛知県立大学, 情報科学部, 准教授 (50324968)
TORIYAMA Michinori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (90457151)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 脳・神経 / 発生・分化 / 神経科学 / 軸索輸送 / 細胞骨格 / 分子クラッチ / Shootin1 / アクチン線維 |
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| Research Abstract |
Although actin and associated proteins are essential for axonal extension, how they are transported along axons remains unclear. Here we show that actin filaments (F-actins) and associated proteins migrate toward the axonal growth cone by means of directional actin polymerization/depolymerization, called treadmilling. F-actins migrating along axonal shafts underwent treadmilling, with their polymerizing ends oriented toward the growth cone. F-actins were anchored to the plasma membrane through the cell adhesion molecule L1-CAM and the linker protein shootin1. Migration of F-actins depended on their polymerization and substrate anchorage, and directional counter-forces associated with F-actin migration were detected on the substrate. Actin-associated proteins co-migrated with F-actins by interacting with the filaments. Our findings reveal a new mode of intracellular transport, which supplies actin and associated proteins to the axonal leading edge, thereby promoting its protrusion.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Ceramic coating of liposomal gene carrier for minimizing toxicity to primary hippocampal neurons2013
Author(s)
Tahara, K., Moriuchi, T., Tsukui, M., Hirota, A., Maeno, T., Toriyama, M., Inagaki, N. and Kikuchi, J
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Journal Title
Chemistry Letters
Volume: 42
Pages: 1265-1267
NAID
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Peer Reviewed
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[Presentation] Cortactin functions as a clutch molecule to promote axon outgrowth
Author(s)
Kubo, Y., Toriyama, M., Kozawa, S., Ikeda, K., Sugiura, T. and Inagaki, N.
Organizer
International Symposium on “Sensory Systems and Neural Circuits”
Place of Presentation
東京大学本郷キャンパス
Related Report
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[Presentation] Cortactin functions as a clutch molecule to promote axon outgrowth
Author(s)
Kubo, Y., Toriyama, M., Kozawa, S., Ikeda, K., Sugiura, T., and Inagaki, N.
Organizer
2012 Annual Meeting of the American Society for Cell Biology
Place of Presentation
San Francisco, USA
Related Report
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[Presentation] Slow axonal transport driven by directional actin treadmilling
Author(s)
Katsuno, H., Toriyama, M., Sakumura, Y., Ikeda, K. Mizuno, K., and Inagaki, N.
Organizer
2012 Annual Meeting of the American Society for Cell Biology
Place of Presentation
San Francisco, USA
Related Report
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[Presentation] Slow axonal transport driven by directional actin treadmilling of anchored actin filaments
Author(s)
Katsuno, H., Toriyama, M., Sakumura, Y., Ikeda, K. Mizuno, K., and Inagaki, N.
Organizer
第35回 日本神経科学大会
Place of Presentation
愛知県名古屋市
Related Report
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[Presentation] Cortactin functions as a clutch molecule to promote axon outgrowth
Author(s)
Kubo, Y., Toriyama, M., Kozawa, S., Ikeda, K., Sugiura, T., and Inagaki, N.
Organizer
第45回日本発生生物学会・第64回日本細胞生物学会合同大会
Place of Presentation
兵庫県神戸市
Related Report
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