| Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Outline of Final Research Achievements |
In this study, we have developed a method to isolate cell cortices from oocytes and eggs of the anuran amphibian Xenopus laevis. Using isolated cortices of Xenopus oocytes and eggs, we investigated the protein components of cell cortices of immature oocytes and mature eggs. We have found that the amount of anillin, an actin-binding protein, increased more than twice during oocyte maturation. To know the physiological meaning of the increase in the anillin amount, the newly synthesis of anilline during oocyte maturation was inhibited by the use of antisense oligonucleotides. We also inhibited the anillin function with anti-anillin antibodies. The results demonstrated that the increased anillin is required for the formation of the first polar body and also for cleavage division after fertilization. Thus, it is suggested that the cell cortex should change during oocyte maturation to render oocytes more competent in undergoing cytokinesis.
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