Analysis of regulatory mechanisms of epigenetic gene regulation by a complex of nuclear matrix protein and noncoding RNA
| Project/Area Number |
23370093
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
NAKAGAWA Shinichi 独立行政法人理化学研究所, 中川RNA生物学研究室, 准主任研究員 (50324679)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2013: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 核マトリクス / hnRNP U / インプリンティング / アリル特異的発現 / ノンコーディングRNA / Xist / X染色体 / ゲノムインプリンティング / Airn / Kcnqlot1 / クロマチン免疫沈降 / クロスリンク免疫沈降 |
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| Research Abstract |
In the human and mouse, vast majority of the genome is transcribed into RNAs, producing a huge number of non protein-coding RNAs. These noncoding RNAs associate with chromatin-modifying complexes, and are assumed to regulate epigenetic gene expression. In this study, we have performed functional analysis of a nuclear matrix protein hnRNP U that regulate chromosomal localization of Xist, a renowned noncoding RNA involved in the X chromosome inactivation. We found that hnRNP U also regulates the chromosomal localization and function of noncoding RNAs such as Kcnq1ot1 and Airn, and the allele-specific expression of the imprinted loci were impaired upon depletion of hnRNP U. We also performed genome-wide gene expression analysis of the hnRNP U depleted cells, and found that multiple allele-specific expression were also disturbed. We found a novel noncoding RNA expressed from the allele-specific locus, and its transcripts were also associated with the chromosome in a mono-allelic manner.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Malat1 is not an essential component of nuclear speckles in mice2012
Author(s)
Nakagawa, S., Ip, J. Y., Shioi, G., Tripathi, V., Zong, X., Hirose, T., and Prasanth, K. V
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Journal Title
RNA
Volume: 18
Issue: 8
Pages: 1487-1499
DOI
Related Report
Peer Reviewed
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[Journal Article] Alternative 3'-end processing of long noncoding RNA initiates construction of nuclear paraspeckles2012
Author(s)
Naganuma, T., Nakagawa, S., Tanigawa, A., Sasaki, Y. F., Goshima, N., and Hirose, T
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Journal Title
EMBO J
Volume: 31
Issue: 20
Pages: 4020-4034
DOI
Related Report
Peer Reviewed
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[Journal Article] Competition between a noncoding exon and introns : Gomafu contains tandem UACUAAC repeats and associates with splicing factor-12011
Author(s)
Tsuiji, H., Yoshimoto, R., Hasegawa, Y., Furuno, M., Yoshida, M., and Nakagawa, S
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Journal Title
Genes Cells
Volume: 16
Issue: 5
Pages: 479-490
DOI
Related Report
Peer Reviewed
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