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Chromosome evolution in Lepidoptera analyzed by comparative FISH mapping

Research Project

Project/Area Number 23380030
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Insect science
Research InstitutionIwate University (2012-2014)
Hokkaido University (2011)

Principal Investigator

SAHARA Ken  岩手大学, 農学部, 教授 (30241368)

Co-Investigator(Kenkyū-buntansha) SUZUKI Go  大阪教育大学, 教育学部, 教授 (10314444)
安河内 祐二  独立行政法人農業生物資源研究所, その他部局等, 研究員 (50355723)
Co-Investigator(Renkei-kenkyūsha) YASUKOCHI Yuji  独立行政法人農業生物資源研究所, その他部局等, 研究員 (50355723)
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords染色体進化 / FISH / オルソログ / カイコゲノム / BAC-FISH / 遺伝子マッピング / ヒゲナガカワトビケラ / ゲノム比較 / コウモリガ / BAC-FIS / コナガ / オオモンシロチョウ / cDNA / BAC
Outline of Final Research Achievements

We obtained information of Bombyx mori single orthologs from Plutella xylostella, Endoclita excrescens and Stenopsyche marmorata by means of EST analyses. BAC libraries in the latter two species were also constructed in the present study. The BAC-FISH experimental system in these species were successfully developed to analyze chromosome correspondence to the model Lepidoptera, the silkworm. Comprehensive genome comparison between B. mori and the target species revealed that conserved synteny of gene order is apparent in P. xylostella. While a trichopteran representative, S. marmorata showed only putative microsyntenic correspondence to the gene order of the silkworm. Pieris does not have conserved synteny in spite of well conserved correspondence between the two species.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (27 results)

All 2015 2014 2013 2012 2011

All Journal Article (15 results) (of which Peer Reviewed: 14 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (10 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] (2015): A new molecular technique for determining the sex of Harmonia axyridis2015

    • Author(s)
      Gotoh H, Nishikawa H, Sahara K, Yaginuma T, Niimi T
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 84

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] チョウ目昆虫の性染色体進化2015

    • Author(s)
      佐原 健・松橋伊織・吉戸敦生
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 84

    • NAID

      130005087597

    • Related Report
      2014 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Plant omics data center: an integrated web repository for interspecies gene expression networks with NLP-based curation.2015

    • Author(s)
      Ohyanagi, H., Takano, T., Terashima, S., Kobayashi, M., Kanno, M., Morimoto, K., Matsumura, H., Sasaki, Y., Aya, K., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., Yokoyama, K., Yano, K.
    • Journal Title

      Plant and Cell Physiology

      Volume: 56 Issue: 1 Pages: 1-8

    • DOI

      10.1093/pcp/pcu188

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The LOSS OF APOMEIOSIS (LOA) locus in Hieracium praealtum can function independently of the associated large-scale repetitive chromosomal structure.2014

    • Author(s)
      Kotani, Y., Henderson, S.T., Suzuki, G., Johnson, S.D., Okada, T., Siddons, H., Mukai, Y., Koltunow, A.M.G.
    • Journal Title

      New Phytologist

      Volume: 201 Issue: 3 Pages: 973-981

    • DOI

      10.1111/nph.12574

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FISH identification of Helicoverpa armigera and Mamestra brassicae chromosomes by BAC and fosmid probes.2013

    • Author(s)
      Sahara K, Yoshido A, Shibata F, Fujikawa-Kojima N, Okabe T, Tanaka-Okuyama M, Yasukochi Y.
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 43 Issue: 8 Pages: 644-653

    • DOI

      10.1016/j.ibmb.2013.04.003

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid turnover of the W chromosome in geographical populations of wild silkmoths, Samia cynthia ssp2013

    • Author(s)
      Yoshido A, Sichova J, Kubichova S, Marec F, Sahara K
    • Journal Title

      Chromosome Research

      Volume: 21 Issue: 2 Pages: 149-164

    • DOI

      10.1007/s10577-013-9344-1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neo-sex chromosomes and adaptive potential in tortricid pests2013

    • Author(s)
      Nguyen P, Sykorova M, Sichova J, Kuta V, Dalikova M, Capkova-Frydrychva R, Neven LG, Sahara K, Marec F
    • Journal Title

      PNAS

      Volume: 110 Issue: 17 Pages: 6931-6936

    • DOI

      10.1073/pnas.1220372110

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular localization of mitotic RAD21 with repetitive amino acid motifs in Allium cepa.2013

    • Author(s)
      Suzuki, G., Nishiuchi, C., Tsuru, A., Kako, E., Li, J., Yamamoto, M., Mukai, Y.
    • Journal Title

      Gene

      Volume: 514 Issue: 2 Pages: 75-81

    • DOI

      10.1016/j.gene.2012.11.012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EST sequencing and fosmid library construction in a non-model moth, Mamestra brassicae, for comparative mapping2012

    • Author(s)
      Kamimura M, Taetishi K, Tanaka-Okuyama M, Okabe T, Shibata F, Sahara K, Yasukochi Y
    • Journal Title

      Genome

      Volume: 55 Issue: 11 Pages: 775-781

    • DOI

      10.1139/g2012-063

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Random BAC FISH of monocot plants reveals differential distribution of repetitive DNA elements in small and large chromosome species2012

    • Author(s)
      Suzuki, G., et al
    • Journal Title

      Plant Cell Report

      Volume: 31 Issue: 4 Pages: 621-628

    • DOI

      10.1007/s00299-011-1178-8

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Step-by-step evolution of neo-sex chromosomes in geographical populations of wild silkmoth, Samia cynthia ssp.2011

    • Author(s)
      吉戸敦生
    • Journal Title

      Heredity

      Volume: 106 Issue: 4 Pages: 614-624

    • DOI

      10.1038/hdy.2010.94

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of BAC clones containing conserved genes from libraries of three distantly related moths: A useful resource for comparative genomics of Lepidoptera2011

    • Author(s)
      Yasukochi Y, Tanaka-Okuyama M, Kamimura M, Nakano R, Naito Y, Ishikawa Y, Sahara K
    • Journal Title

      Journal of Biomedicine and Biotechnology

      Volume: 2011 Issue: 1 Pages: 165894-165894

    • DOI

      10.1155/2011/165894

    • NAID

      120003043350

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Samia cynthia versus Bombyx mori : Comparative gene mapping between a species with a low-number karyotype and the model2011

    • Author(s)
      Yoshido A, Yasukochi Y, Sahara K
    • Journal Title

      Insect Biochem Mol Biol

      Volume: 41 Issue: 6 Pages: 370-377

    • DOI

      10.1016/j.ibmb.2011.02.005

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sex-linked pheromone receptor genes of the European corn borer, Ostrinia nubilalis, are in tandem arrays2011

    • Author(s)
      Yasukochi Y, Miura N, Nakano R, Sahara K, Ishikawa Y
    • Journal Title

      PLoS ONE

      Volume: 6 Issue: 4 Pages: 18843-18843

    • DOI

      10.1371/journal.pone.0018843

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sex chromosome evolution in moths and buttertflies2011

    • Author(s)
      Sahara K, Yoshido A, Traut
    • Journal Title

      Chromosome Res

      Volume: 20 Issue: 1 Pages: 83-94

    • DOI

      10.1007/s10577-011-9262-z

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 染色体異系統を用いてカイコ突然変異の原因遺伝子にせまる2015

    • Author(s)
      佐原 健・鈴木一生・安河内祐二・伴野 豊
    • Organizer
      第59回応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 毛翅目昆虫ヒゲナガカワトビケラのBAC-FISHマッピング2015

    • Author(s)
      藤本章晃・吉戸敦生・鈴木剛・安河内祐二・ 佐原健
    • Organizer
      第59回応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヨーロッパアワノメイガとカイコとの染色体比較から見えてくるもの2015

    • Author(s)
      大野瑞紀・柴田洋・石川幸男・中野亮・安河内祐二・佐原健
    • Organizer
      第59回応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] FISH によるn=28とn=31の鱗翅目昆虫染色体対応関係の解析2014

    • Author(s)
      大野瑞紀・河合綾・藤川-小島典子・柴田洋・安河内祐二・佐原健
    • Organizer
      染色体学会 第65回年会
    • Place of Presentation
      倉敷市芸文館
    • Year and Date
      2014-10-24 – 2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラン科植物Rhynchostylis属とNeofinetia属の細胞遺伝学的解析のためのBACライブラリー構築.2014

    • Author(s)
      松葉篤・藤井美樹・鈴木剛・山本真紀・向井康比己
    • Organizer
      染色体学会 第65回年会
    • Place of Presentation
      倉敷市芸文館
    • Year and Date
      2014-10-24 – 2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 2n=55のカイコ個体2014

    • Author(s)
      佐原健・鈴木宙・石井啓祐・山田恭裕・安河内祐二
    • Organizer
      日本蚕糸学会第84回大会
    • Place of Presentation
      神奈川県藤沢市亀井野1866 日本大学生物資源科学部
    • Related Report
      2013 Annual Research Report
  • [Presentation] Incest avoidance applicable in hybrid seed production.2013

    • Author(s)
      Suzuki, G., Takada, Y., and Watanabe, M.
    • Organizer
      International Symposium on Diversifying Biological Resources. -Toward Food Security and Sustainable Society-
    • Place of Presentation
      University of Tsukuba, Tsukoba, Japan
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] アワノメイガとウスジロキノメイガの性フェロモン受容体遺伝子クラスターの構造解析2013

    • Author(s)
      安河内祐二
    • Organizer
      日本蚕糸学会第83回大会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所(茨城県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シンジュ蚕W染色体はランダムに配分されることがある2013

    • Author(s)
      吉戸敦生・佐原健
    • Organizer
      日本蚕糸学会第83回大会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所(茨城県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シンジュサンにおける染色体の同定と性染色体進化の解明2011

    • Author(s)
      吉戸敦生・安河内祐二・○佐原健
    • Organizer
      日本染色体学会
    • Place of Presentation
      神奈川大学平塚キャンパス
    • Year and Date
      2011-11-11
    • Related Report
      2011 Annual Research Report
  • [Book] Chapter 6: Silkmoths (Lepidoptera). In. Protocols for cytogenetic mapping of arthropod genomes. pp219-256.2014

    • Author(s)
      Yoshido A, Sahara K, Yasukochi Y
    • Total Pages
      526
    • Publisher
      CRC press
    • Related Report
      2014 Annual Research Report
  • [Book] 海游舎2012

    • Author(s)
      齋藤裕・佐原健 共編
    • Total Pages
      195
    • Publisher
      糸の博物誌 ムシたちが糸で織りなす多様な世界
    • Related Report
      2012 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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