| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Research Abstract |
Pacific abalone is one of the most important aquaculture species in the Tohoku and Hokkaido areas. The most crucial problem for abalone culture is the characteristically slow growth. In an attempt to understand growth regulation in the abalone, we tried to determined growth-stimulating peptide.
cDNA libraries of the cerebral ganglions of abalone were prepared using total RNAs, and about 3300 colons were used for the sequence analysis. At least 42 peptide homologs were detected, and large number of clone is Neuropeptide Y (NPY) homolog. Two NPY cDNAs and peptides, named abNPY-1 and 2, were determined by cDNA cloning and LC-MS/MS.Higher AbNPY mRNA levels were observed in the larger-sized abalone than in those of smaller abalone. Injection of abNPY to juvenile abalone resulted in an increase in body mass. These results suggest that NPY homolog is present in the cerebral ganglion and this peptide is involved in the regulation of somatic growth of the Pacific abalone.
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