Research on the identification of growth-stimulating peptides from the cerebral ganglion of Pacific abalone
| Project/Area Number |
23380114
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKUMURA Seiichi 北里大学, 海洋生命科学部, 准教授 (60224169)
AMANO Haruna 北里大学, 海洋生命科学部, 講師 (50431341)
UCHIDA Katsuhisa 宮崎大学, 農学部, 准教授 (50360508)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Keywords | エゾアワビ / 脳神経節cDNAライブラリー / 食欲調節ペプチド / 再生促進ペプチド / 性成熟促進ペプチド / 代謝調節ペプチド / 受容体 / 成長促進ペプチド / アワビ |
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| Research Abstract |
Pacific abalone is one of the most important aquaculture species in the Tohoku and Hokkaido areas. The most crucial problem for abalone culture is the characteristically slow growth. In an attempt to understand growth regulation in the abalone, we tried to determined growth-stimulating peptide.
cDNA libraries of the cerebral ganglions of abalone were prepared using total RNAs, and about 3300 colons were used for the sequence analysis. At least 42 peptide homologs were detected, and large number of clone is Neuropeptide Y (NPY) homolog. Two NPY cDNAs and peptides, named abNPY-1 and 2, were determined by cDNA cloning and LC-MS/MS.Higher AbNPY mRNA levels were observed in the larger-sized abalone than in those of smaller abalone. Injection of abNPY to juvenile abalone resulted in an increase in body mass. These results suggest that NPY homolog is present in the cerebral ganglion and this peptide is involved in the regulation of somatic growth of the Pacific abalone.
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Report
(4 results)
Research Products
(73 results)
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[Journal Article] Involvement of insulin-like peptide in long-term synaptic plasticity and long-term memory of the pond snail Lymnaea stagnalis2013
Author(s)
Murakami J, Okada R, Sadamoto H, Kobayashi S, Mita K, Sakamoto Y, Yamagishi M, Hatakeyama D, Otsuka E, Okuta A, Sunada H, Takigami S, Sakakibara M, Fujito Y, Awaji M, Moriyama S, Lukowiak K, Ito E
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Journal Title
J Neurosc
Volume: 33
Issue: 1
Pages: 371-381
DOI
Related Report
Peer Reviewed
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[Journal Article] Species sensitivity distribution approach to primary risk analysis of the metal pyrithione photodegradation product, 2,2’-dipyridyldisulfide in the Inland Sea and induction of notochord undulation in fish embryos.2012
Author(s)
Mochida K, Amano H, Ito K, Ito M, Onduka T, Ichihashi H, Kakuno A, Harino H, Fujii K.
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Journal Title
Aquat Toxicol
Volume: 118
Pages: 152-163
DOI
Related Report
Peer Reviewed
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[Book] Prolactin2013
Author(s)
Takahashi H, Kudose H, Takagi C, Moriyama S, Sakamoto T.
Publisher
InTech
Related Report
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