Adjuvant effect of chemicals on contact hypersensitivity through transient receptor potential channels

• Project/Area Number: 23390031
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: University of Shizuoka
• Principal Investigator: IMAI Yasuyuki 静岡県立大学, 薬学部, 教授 (80160034)
• Co-Investigator(Kenkyū-buntansha): KUROHANE Kohta 静岡県立大学, 薬学部, 助教 (90333525)
• Co-Investigator(Renkei-kenkyūsha): WATANABE Tatsuo 静岡県立大学, 食品栄養科学部, 教授 (10210915)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: フタル酸エステル / 接触性皮膚炎 / TRPチャネル / 知覚神経 / 代替可塑剤 / アジュバント / アレルギー・ぜんそく / 衛生 / 免疫学 / 薬学 / 神経科学

Research Abstract

Adjuvant mechanisms of chemicals in allergy are largely unknown. We demonstrated that TRPA1 channels are involved in the adjuvant activity of phthalate esters using a contact hypersensitivity mouse model. This conclusion was supported by the presence of adjuvant activity in TRPA1 agonistic natural products as well as by experiments using a TRPA1 antagonist. TRPA1 activation was found to be useful for the prediction of adjuvant effect using alternative plasticizers that are widely used for cosmetics and drug formulation.

Research Products

• [Journal Article] Lack of transient receptor potential melastatin 8 activation by phthalate esters that enhance contact hypersensitivity in mice (2013)
  • Author(s): Kohta Kurohane, Yurina Sahara, Ayako Kimura, Masataka Narukawa, Tatsuo Watanabe, Takashi Daimon, Yasuyuki Imai
  • Journal Title: Toxicol. Lett Volume: 217 Issue: 3 Pages: 192-196
  • DOI: 10.1016/j.toxlet.2012.12.025
  • Peer Reviewed
• [Journal Article] Transient receptor potential ankyrin 1 activation enhances hapten sensitization in a T-helper type 2-driven fluorescein isothiocyanate -induced contact hypersensitivity mouse model (2012)
  • Author(s): Takahiro Shiba, Takuma Tamai, Yurina Sahara, Kohta Kurohane, Tatsuo Watanabe, Yasuyuki Imai
  • Journal Title: Toxicol. Appl. Pharmacol Volume: 264 Issue: 3 Pages: 370-376
  • DOI: 10.1016/j.taap.2012.08.012
  • Peer Reviewed
• [Journal Article] High dose dietary pyridoxine induces T-helper type 1 polarization and decreases contact hypersensitivity response to fluorescein isothiocyanate in mice (2012)
  • Author(s): Chie Kobayashi, Kohta Kurohane, Yasuyuki Imai
  • Journal Title: Biol. Pharm. Bull Volume: 35 Pages: 532-538
  • NAID: 130001872201
  • URL: https://www.jstage.jst.go.jp/article/bpb/35/4/35_4_532/_article
  • Peer Reviewed
• [Journal Article] High dose dietary pyridoxine induces T-helper type 1 polarization and decreases contact hypersensitivity response to fluorescein isothiocyanate in mice (2012)
  • Author(s): Chie Kobayashi
  • Journal Title: Biol.Pharm.Bull. Volume: 35 Pages: 532-538
  • Peer Reviewed
• [Presentation] 接触性皮膚炎の感作過程における代替可塑剤のアジュバント作用機構 (2013)
  • Author(s): 寺澤里恵, 木村文子, 小林香美結, 鈴木若菜, 松岡 毅, 黒羽子孝太, 今井康之
  • Organizer: 日本病院薬剤師会東海ブロック/日本薬学会東海支部合同学術大会2013
  • Place of Presentation: 鈴鹿市
  • Year and Date: 2013-11-10
• [Presentation] 接触性皮膚炎の感作過程における代替可塑剤のアジュバント作用機構 (2013)
  • Author(s): 寺澤里恵、木村文子、小林香美結、鈴木若菜、松岡 毅、黒羽子孝太、今井康之
  • Organizer: 日本病院薬剤師会東海ブロック／日本薬学会東海支部合同学術大会2013
  • Place of Presentation: 鈴鹿市
• [Presentation] FITC 誘導接触性皮膚炎における代替可塑剤のアジュバント活性 (2012)
  • Author(s): 木村文子, 佐原百合名, 黒羽子孝太, 渡辺達夫, 今井康之
  • Organizer: 第58回日本薬学会東海支部総会・大会
  • Place of Presentation: 静岡市
  • Year and Date: 2012-07-07
• [Presentation] 接触性皮膚炎に対するフタル酸エステル類のアジュバント活性におけるTRP channel の関与 (2012)
  • Author(s): 黒羽子孝太, 佐原百合名, 木村文子, 玉井琢磨, 渡辺達夫, 今井康之
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-03-29
• [Presentation] 接触性皮膚炎に対するフタル酸エステル類のアジュバント活性におけるTRP channelの関与 (2012)
  • Author(s): 黒羽子孝太
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] FITC誘導接触性皮膚炎における代替可塑剤のアジュバント活性 (2012)
  • Author(s): 木村 文子
  • Organizer: 第58回日本薬学会東海支部総会・大会
  • Place of Presentation: 静岡市
• [Presentation] Lack of TRPM8 involvement in the enhancement of skin sensitization to FITC (2011)
  • Author(s): Yurina Sahara, Kohta Kurohane, Yasuyuki Imai
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 千葉市
  • Year and Date: 2011-11-27
• [Presentation] Lack of TRPM8 involvement in the enhancement of skin sensitization to FITC (2011)
  • Author(s): 佐原百合名
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 幕張(千葉)
  • Year and Date: 2011-11-27
• [Remarks]
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/bisei/
• [Remarks] 研究室のホームページ
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/bisei/
• [Remarks] 研究室英語ホームページ
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/bisei/English/index.html
• [Remarks] 研究室のホームページ
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/bisei/
• [Remarks] 研究室の英語ホームページ
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/bisei/English/index.html
• [Remarks]
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/bisei/