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Validation of therapeutic efficacy of allogenic transplantation therapy using pluripotent stem stem cell-derived cardiomyocytes for myocardial infarction in non-human primate: common marmoset.

Research Project

Project/Area Number 23390072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKeio University

Principal Investigator

HATTORI Fumiyuki  慶應義塾大学, 医学部, 講師(非常勤) (50398624)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Hiromi  慶應義塾大学, 医学部, 特任助教 (30594890)
OUGINO Yasuyuki  慶應義塾大学, 医学部, 助教 (20598916)
Co-Investigator(Renkei-kenkyūsha) SASAKI Erika  公益財団法人実験動物中央研究所, 応用発生学研究部, 部長 (70390739)
ITO Toshio  公益財団法人実験動物中央研究所, マーモセット研究部, 部長 (20106644)
Project Period (FY) 2011-11-18 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords再生医学 / 心筋細胞 / 多能性幹細胞 / 心臓再生 / ES細胞 / 同種移植 / 霊長類 / 心筋梗塞モデル / 再生医療
Research Abstract

We demonstrated myocardial infarctions in common marmoset and simultaneously transplanted saline (Sham group: n=5) and 1 x 10^6 purified cardiomyocyte-balls (Treated group: n=5) into their myocardium. We performed monthly echocardiographic analyses before and after the surgeries until three months of them, which revealed that only after one month of the surgery, heart functions of the treated group were significantly sustained compared with sham group. After three month of the transplantation, histological analyses showed good survival of GFP-positive graft cardiomyocytes in the infracted area and border zone. The infarct/total heart volume was no significant difference between sham and treated groups. In conclusion, allogenic transplant of embryonic stem cell-derived cardiomyocytes served a significant but transient beneficial effect to the heart infracted model developed in a non-human primate, common marmoset.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (5 results)

All 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results) (of which Invited: 1 results)

  • [Journal Article] Distinct Metabolic Flow Enable Large-Scale Purification of Mouse and Human Pluripotent Stem Cell-Derived Cardiomyocytes.2013

    • Author(s)
      Tohyama S, Hattori F, Sano M, et al.
    • Journal Title

      Cell Stem Cell

      Volume: 12 Issue: 1 Pages: 127-37

    • DOI

      10.1016/j.stem.2012.09.013

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct iPS Cells shows different Cardiac Differentiation Efficiency.2013

    • Author(s)
      Ohno Y, Yuasa S, Seki T, Fukuda K., et al.
    • Journal Title

      Stem Cells International

      Volume: 2013 Pages: 659739-659739

    • DOI

      10.1155/2013/659739

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes2013

    • Author(s)
      Shugo Tohyama
    • Journal Title

      Cell Stem Cell

      Volume: 12 Pages: 127-137

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] iPS細胞を活用した心臓再生医療と創薬応用に向けた現状と今後2013

    • Author(s)
      服部文幸
    • Organizer
      日本学術会議 薬学委員会 生物系薬学分科会シンポジウム
    • Place of Presentation
      日本学術会議講堂
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Progress of a heart cell-therapy model system in Common Marmoset using Embryonic stem cells2012

    • Author(s)
      服部文幸,山下裕美
    • Organizer
      日本マーモセット研究会
    • Place of Presentation
      東京医科歯科大学 鈴木章夫記念講堂
    • Related Report
      2013 Final Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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