Analysis of molecular mechanisms underlying retinal photoreceptor cell fate determination
| Project/Area Number |
23390074
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University (2012-2013)
Osaka Bioscience Institute (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 網膜 / 視細胞 / 網膜変性症 / ノックアウトマウス / 遺伝子制御 / 中枢神経系 / 細胞運命 / 光受容体 |
|---|
| Research Abstract |
In vertebrate retinal development, it has been known that six types of neurons and glial cells (photoreceptors, horizontal cells, bipolar cells, amacrine cells, ganglion cells, and Müller glial cells) are generated from common multipotent progenitor cells. In this developmental process, the gene expression programs controlling retinal development need to be precisely regulated by activating the expression of proper genes and repressing the expression of improper genes. We analyzed the molecular mechanisms underlying this genetic program using mouse molecular genetics. In the current project, we clarified several important mechanisms in cell fate determination, epigenetic regulation, maturation, and survival of photoreceptors. In addition, we developed a gene therapy system using AAV (adenovirus-associated virus), which is a important clue to the suitability of gene therapy for developmental disorders and degeneration of the retina in humans.
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Report
(4 results)
Research Products
(101 results)
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[Journal Article] Histone demethylase Jmjd3 is required for the development of subsets of retinal bipolar cells.2014
Author(s)
Iida A, Iwagawa T, Kuribayashi H, Satoh S, Mochizuki Y, Baba Y, Nakauchi H, Furukawa T, Koseki H, Murakami A, Watanabe S.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 111
Issue: 10
Pages: 3751-6
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Targeted ablation of Crb2 in Photoreceptor Cells induces Retinitis Pigmentosa.2014
Author(s)
Alves CH, Pellissier LP, Vos RM, Garcia Garrido M, Sothilingam V, Seide C, Beck SC, Klooster J, Furukawa T, Flannery JG, Verhaagen J, Seeliger MW & Wijnholds J
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Journal Title
Hum. Mol. Genet
Volume: 印刷中
Issue: 13
Pages: 3384-3401
DOI
Related Report
Peer Reviewed
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[Journal Article] Epigenetics of eu- and heterochromatin in inverted and conventional nuclei.2013
Author(s)
Eberhart A, Feodorava Y, Song C, Wanner G, Kiseleva E, Hake S, Furukawa T, Kimura H, Schotta G, Leonhardt H, Joffe B & Solovei I
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Journal Title
Chromosome Res.
Volume: 20
Issue: 5
Pages: 535-554
DOI
Related Report
Peer Reviewed
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[Journal Article] Basolateral Mg2+ extrusion via CNNM4 mediates transcellular Mg2+ transport across epithelia: a mouse model2013
Author(s)
Daisuke Yamazaki, Yosuke Funato, Jiro Miura, Sunao Sato, Satoru Toyosawa, Kazuharu Furutani, Yoshihisa Kurachi, Yoshihiro Omori, Takahisa Furukawa, Tetsuya Tsuda, Susumu Kuwabata, Shin Mizukami, Kazuya Kikuchi, Hiroaki Miki
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Journal Title
PLoS Genetics
Volume: 9(12)
Issue: 12
Pages: e1003983-e1003983
DOI
Related Report
Peer Reviewed
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[Journal Article] miR-124a is required for hippocampal axogenesis and retinal cone survival through Lhx2 suppression2011
Author(s)
Sanuki R, Onishi A, Koike C, Muramatsu R, Watanabe S, Muranishi Y, Irie S, Uneo S, Koyasu T, Matsui R, Cherasse Y, Urade Y, Watanabe D, KondoM, Yamashita T, Furukawa T
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Journal Title
Nat Neurosci
Volume: 14
Issue: 9
Pages: 1125-1134
DOI
Related Report
Peer Reviewed
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[Journal Article] Analysis of transcriptional regulatory pathways of photoreceptor genes by expression profiling of the Otx2-deficient retina2011
Author(s)
Omori, Y., Katoh, K., Sato, S., Muranishi, Y., Chaya, T., Onishi, A.(代表), Minami, T., Fujikado, T., Furukawa, T.
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Journal Title
PLoS One
Volume: 6(5)
Issue: 5
Pages: e19685-e19685
DOI
Related Report
Peer Reviewed
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[Presentation] RAX Homeoprotein and NOTCH-HES Signaling Regulate Otx2 Expression in Embryonic Retinal Photoreceptor Cell Fate Determination2012
Author(s)
Takahisa Furukawa , Yuki Muranishi, Koji Terada, Tatsuya Inoue, Kimiko Katoh, Toshinori Tsujii, Rikako Sanuki, Yasuhiro Tamaki
Organizer
ARVO2012
Place of Presentation
Convention Center, Fort Lauderdale, Florida, USA
Year and Date
2012-05-08
Related Report
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[Presentation] Regulation of cilia formation by ciliary kinases2012
Author(s)
Takahisa Furukawa, Yoshihiro Omori, Taro Chaya, Kimiko Katoh
Organizer
14th Vision Research Conference, Retina Ciliopathies-From genes to mechanisms and treatment
Place of Presentation
Convention Center, Fort Lauderdale, Florida, USA
Year and Date
2012-05-04
Related Report
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