Establishment of early diagnosis system of pancreatobiliary carcinomas by the application of mucin expression profiles
| Project/Area Number |
23390085
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
YONEZAWA Suguru 鹿児島大学, 医歯(薬)学総合研究科, 教授 (10175002)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIGASHI Michiyo 鹿児島大学, 医歯学総合研究科, 准教授 (60315405)
YOKOYAMA Seiya 鹿児島大学, 医歯学総合研究科, 助教 (20569941)
SHINCHI Hiroyuki 鹿児島大学, 医歯学総合研究科, 教授 (60284874)
NARIMATSU Hisashi 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, センター長 (40129581)
KAMEYAMA Akihiko 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, チーム長 (80415661)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
|
|---|
| Keywords | 膵癌 / IPMN / mucin / DNA methylation / diagnosis / angiogenesis / 膵胆管系腫瘍 / ムチン / DNAメチル化 / 異常糖鎖修飾 / intestinal-type IPMN / gastric-type IPMN / 悪性度 / MUC4 / MUC1 / 低酸素応答性 / 膵胆管系癌 / MUC16 / MUC17 / 予後 / DANメチル化 |
|---|
| Research Abstract |
It is very difficult to perform an early detection of pancreatic ductal adenocarcinomas (PDACs) or an accurate differential diagnosis for subtypes of intraductal papillary mucinous neoplasms (IPMNs) with different outcome. Expression profiles of MUC1, MUC2 and MUC4 are different between PDACs and IPMNs. We developed a methylation-specific electrophoresis (MSE) method to analyze the DNA methylation status.
By using the MSE, we evaluated pancreatic juices from 45 patients. The predictive diagnosis by MSE showed sensitivity and specificity of 80% and 87% for PDAC; 88% and 100% for intestinal-type IPMN; and 77% and 88% for gastric-type IPMN; respectively. The MSE analysis of pancreatic juices may give us useful informations for diagnosis of human pancreatic neoplasms. Another study for the first time demonstrated a pivotal role of MUC1 in controlling the hypoxia-driven angiogenesis through the regulation of multiple proangiogenic factors in PDAC cells.
|
Report
(4 results)
Research Products
(65 results)
-
[Journal Article] Diagnosis of pancreatic neoplasms using a novel method of DNA methylation analysis of mucin expression in pancreatic juice2014
Author(s)
Yokoyama S, Kitamoto S, Higashi M, Goto Y, Hara T, Ikebe D, Yamaguchi T, Arisaka Y, Niihara, Nishimata H, Tanaka S, Takaori K, Batra S K, Yonezawa S
-
Journal Title
PLoS ONE
Volume: 9(4)
Issue: 4
Pages: e93760-e93760
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Pathobiological implications of mucin (MUC) expression in the outcome of small bowel cancer2014
Author(s)
Shibahara H, Higashi M, Koriyama C, Yokoyama S, Kitazono I, Yasuhiro K, Narita M, Kuze S, Takanori K, Mita S, Arai T, Kato T, Yuasa N, Yamaguchi R, Kubota H, Suzuki H, Baba S, Rousseau K, Batra S K, Yonezawa S
-
Journal Title
PLoS ONE
Volume: 9(4)
Issue: 4
Pages: e86111-e86111
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Expression of MUC4 mucin is observed mainly in the intestinal-type of intraductal papillary mucinous neoplasm of the pancreas2013
Author(s)
Kitazono I, Higashi M, Kitamoto S, Yokoyama S, Horinouchi M, Osako M, Shimizu T, Tabata M, Batra S K, Goto M, Yonezawa S
-
Journal Title
Pancreas
Volume: 42(7)
Issue: 7
Pages: 1120-1128
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] MUC4 and MUC1 Expression in Adenocarcinoma of the Stomach Correlates with Vessel Invasion and Lymph Node Metastasis : An Immunohisto chemical Study of Early Gastric Cancer.2013
Author(s)
Yukihiro Tamura, Michiyo Higashi, Sho Kitamoto, Seiya Yokoyama, Masahiko Osako, Michiko Horinouchi, Takeshi Shimizu, Mineo Tabata, Surinder K. Batra, Masamichi Goto, Suguru Yonezawa
-
Journal Title
PLoS ONE
Volume: (in press)
Issue: 11
Pages: e49251-e49251
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] The application of new DNA methylation analysis, designated methylation specific electrophoresis (MSE) for pancreatic juice analysis.2012
Author(s)
Yokoyama S, kitamoto S, Hara T, Arisaka Y, Takaori K, Yamamoto T, Niihara T, Nishimata H, Tanaka S, Higashi M, Yonezawa S
Organizer
International Symposium on Pancreas Cancer 2012
Place of Presentation
京都国際コンベンションセンター （京都)
Related Report
-
-
-
-
-
-
-
-
-
[Presentation] ムチンと膵・胆道癌2011
Author(s)
米澤傑
Organizer
第8回日本病理学会カンファレンス
Place of Presentation
ホテルブエナビスタ(長野県)(招待講演)
Year and Date
2011-08-06
Related Report
-
-
-
-
-
-
-
-
-
-
[Book] 病理診断に役立つ分子生物学(病理と臨床臨時増刊号 Vol.29) (分担執筆), 第 2 部 病理診断医になじみのある疾患関連分子, MUC 診断編. (金井弥栄, 石川俊平, 池田栄二;編集)2011
Author(s)
米澤 傑, 山田宗茂, 横山勢也, 北本 祥, 田畑和宏, 東美智代
Total Pages
545
Publisher
株式会社文光堂, 東京
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
