| Project/Area Number |
23390085
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kagoshima University |
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Principal Investigator |
YONEZAWA Suguru 鹿児島大学, 医歯(薬)学総合研究科, 教授 (10175002)
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| Co-Investigator(Kenkyū-buntansha) |
HIGASHI Michiyo 鹿児島大学, 医歯学総合研究科, 准教授 (60315405)
YOKOYAMA Seiya 鹿児島大学, 医歯学総合研究科, 助教 (20569941)
SHINCHI Hiroyuki 鹿児島大学, 医歯学総合研究科, 教授 (60284874)
NARIMATSU Hisashi 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, センター長 (40129581)
KAMEYAMA Akihiko 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, チーム長 (80415661)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Keywords | 膵癌 / IPMN / mucin / DNA methylation / diagnosis / angiogenesis / 膵胆管系腫瘍 / ムチン / DNAメチル化 / 異常糖鎖修飾 / intestinal-type IPMN / gastric-type IPMN / 悪性度 / MUC4 / MUC1 / 低酸素応答性 / 膵胆管系癌 / MUC16 / MUC17 / 予後 / DANメチル化 |
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| Research Abstract |
It is very difficult to perform an early detection of pancreatic ductal adenocarcinomas (PDACs) or an accurate differential diagnosis for subtypes of intraductal papillary mucinous neoplasms (IPMNs) with different outcome. Expression profiles of MUC1, MUC2 and MUC4 are different between PDACs and IPMNs. We developed a methylation-specific electrophoresis (MSE) method to analyze the DNA methylation status.
By using the MSE, we evaluated pancreatic juices from 45 patients. The predictive diagnosis by MSE showed sensitivity and specificity of 80% and 87% for PDAC; 88% and 100% for intestinal-type IPMN; and 77% and 88% for gastric-type IPMN; respectively. The MSE analysis of pancreatic juices may give us useful informations for diagnosis of human pancreatic neoplasms. Another study for the first time demonstrated a pivotal role of MUC1 in controlling the hypoxia-driven angiogenesis through the regulation of multiple proangiogenic factors in PDAC cells.
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