Molecular mechanism of the selective AID targeting at the IgV-region

• Project/Area Number: 23390122
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Kumamoto University
• Principal Investigator: SAKAGUCHI NOBUO 熊本大学, 生命科学研究部, 教授 (70192086)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 免疫学 / ゲノム / 遺伝子

Research Abstract

To elucidate the molecular mechanism of GANP interacted with AID in generation of antibody affinity maturation in B cells, GANP interacting proteins was identified in the nuclear extract from human B cell line Ramos cells. GANP interacts with histones and induces chromatin modification with the acetylation though the GANP HAT domain. GANP binds to the rearranged IgV region. This position is relaxed during IgV transcription. Transcription complex similarly retains during the IgV transcription, which presumably maintains the DNA configuration for continuation and providing the opportunity of AID accession. GANP recruits AID towards the selective site of the rearranged IgV region to augment mutation frequency. We further clarified the role of GANP in the choice of DNA repair pathways after the DNA injury caused by AID at the IgV region gene. GANP is essentially involved in maintenance of DNA and the genome integrity for efficient DNA mutations during antibody affinity maturation.

Research Products

• [Journal Article] GANP regulates the choice of DNA repair pathway by DNA-PKcs interaction in AID-dependent IgV region diversification (2014)
  • Author(s): Eid MMA, Maeda K, Almofty SA, Singh SK, Shimoda M, Sakaguchi N.
  • Journal Title: The Journal of Immunology Volume: 192(印刷中) Issue: 12 Pages: 5529-5539
  • DOI: 10.4049/jimmunol.1400021
  • Peer Reviewed / Open Access
• [Journal Article] Pcid2 inactivates developmental genes in human and mouse embryonic stem cells to sustain their pluripotency by modulation of EID1 stability (2014)
  • Author(s): Ye B, Dai Z, Liu B, Wang R, Li C, Huang G, Wang S, Xia P, Yang X, Kuwahara K, Sakaguchi N, Fan Z
  • Journal Title: Stem Cells Volume: 32 Issue: 3 Pages: 623-635
  • DOI: 10.1002/stem.1580
  • Peer Reviewed
• [Journal Article] Infection of adult thymus with murine retrovirus induces virus-specific central tolerance that prevents functional memory CD8+ T cell differentiation (2014)
  • Author(s): Takamura S, Kajiwara E, Tsuji-Kawahara S, Masumoto T, Fujisawa M, Kato M, Chikaishi T, Kawasaki Y, Kinoshita S, Itoi M, Sakaguchi N, Miyazawa M
  • Journal Title: PLoS Pathog Volume: 10 Issue: 3 Pages: e1003937-e1003937
  • DOI: 10.1371/journal.ppat.1003937
  • Peer Reviewed
• [Journal Article] Histopathological evaluation of the diversity of cells susceptible to H5N1 virulent avian influenza virus (2013)
  • Author(s): Ogiwara H, Yasui F, Munekata K, Takagi-Kamiya A, Munakata T, Nomura N, Shibasaki F, Kuwahara K, Sakaguchi N, Sakoda Y, Kida H, Kohara M
  • Journal Title: Am. J. Pathol Volume: 184 Issue: 1 Pages: 171-183
  • DOI: 10.1016/j.ajpath.2013.10.004
  • Peer Reviewed / Open Access
• [Journal Article] CA-S27 : a novel Lewis a associated carbohydrate epitope is diagnostic and prognostic for cholangiocarcinoma (2013)
  • Author(s): Silsirivanit A, Araki N, Wongkham C, Vaeteewoottacharn K, Pairojkul C, Kuwahara K, Narimatsu Y, Sawaki H, Narimatsu H, Okada S, Sakaguchi N, Wongkham S
  • Journal Title: Cancer Sci Volume: 104 Issue: 10 Pages: 1278-1284
  • DOI: 10.1111/cas.12222
  • Peer Reviewed
• [Journal Article] Broad-spectrum detection of h5 subtype influenza a viruses with a new fluorescent immunochromatography system (2013)
  • Author(s): Sakurai A, Takayama K, Nomura N, Munakata T, Yamamoto N, Tamura T, Yamada J, Hashimoto M, Kuwahara K, Sakoda Y, Suda Y, Kobayashi Y, Sakaguchi N, Kida H, Kohara M, Shibasaki F
  • Journal Title: PLoS One Volume: 8 Issue: 11 Pages: e76753-e76753
  • DOI: 10.1371/journal.pone.0076753
  • Peer Reviewed
• [Journal Article] Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival (2013)
  • Author(s): Rezano A, Kuwahara K, Yamamoto-Ibusuki M, Kitabatake M, Moolthiya P, Phimsen S, Suda T, Tone S, Yamamoto Y, Iwase H, Sakaguchi N
  • Journal Title: BMC Cancer Volume: 13 Issue: 1 Pages: 562-562
  • DOI: 10.1186/1471-2407-13-562
  • Peer Reviewed
• [Journal Article] GANP interacts with APOBEC3G and facilitates its encapsidation into the virions to reduce HIV-1 infectivity (2013)
  • Author(s): Maeda K, Almofty SA (co-first author), Singh SK, Eid MMA, Shimoda M, Ikeda T, Koito A, Pham P, Goodman MF, Sakaguchi N
  • Journal Title: J. Immunol Volume: 191 Issue: 12 Pages: 6030-6039
  • DOI: 10.4049/jimmunol.1302057
  • Peer Reviewed
• [Journal Article] GANP regulates recruitment ofAID to immunoglobulin variable regions bymodulating transcription and nucleosomeoccupancy (2013)
  • Author(s): Shailendra Kumar Singh, Kazuhiko Maeda(co-first author), Mohammed Mansour AbbasEid, Sarah Ameen Almofty, Masaya Ono,Phuong Pham, Myron F. Goodman, and NobuoSakaguchi
  • Journal Title: Nat. Commun Volume: 4 Issue: 1 Pages: 1830-1830
  • DOI: 10.1038/ncomms2823
  • Peer Reviewed
• [Journal Article] Lyn signaling to upregulate GANP is critical for the survival of high-affinity B cells in germinal centers of lymphoid organs (2012)
  • Author(s): Kuwahara K, Nakaya T, Phimsen S, Toda T, Kitabatake M, Kaji T, Takemori T, Watanabe T, Sakaguchi N
  • Journal Title: J. Immunol Volume: 189 Issue: 7 Pages: 3472-3479
  • DOI: 10.4049/jimmunol.1200649
  • Peer Reviewed
• [Journal Article] Transgenic overexpression of G5PR that is normally augmented in centrocytes impairsthe enrichment of high-affinity antigen-specific B cells, increases peritoneal B-1a cells, and induces autoimmunity in aged female mice (2012)
  • Author(s): hi H, Ohtsuji M, Tsurui H, Hirose S, and Sakaguchi N
  • Journal Title: J. Immunol Volume: 189 Issue: 3 Pages: 1193-1201
  • DOI: 10.4049/jimmunol.1102774
  • Peer Reviewed
• [Journal Article] Dynamic appearance of antigenic epitopes effective for viral neutralization during membrane fusion initiated by interactions between HIV-1 envelope proteins and CD4/CXCR4 (2012)
  • Author(s): Toda T, et al
  • Journal Title: Immunobiology Volume: (印刷中) Issue: 9 Pages: 864-872
  • DOI: 10.1016/j.imbio.2011.12.007
  • Peer Reviewed
• [Journal Article] Selective cell death of p53-insufficient cancer cells is induced by knockdown of the mRNA export molecule GANP (2012)
  • Author(s): Phimsen S, et al
  • Journal Title: Apoptosis Volume: (印刷中) Issue: 7 Pages: 679-690
  • DOI: 10.1007/s10495-012-0711-8
  • Peer Reviewed
• [Journal Article] A novel serum carbohydrate marker on mucin 5AC : values for diagnostic and prognostic indicators for cholangiocarcinoma (2011)
  • Author(s): Silsirivanit A, et al
  • Journal Title: Cancer Volume: 117 Issue: 15 Pages: 3393-3403
  • DOI: 10.1002/cncr.25912
  • Peer Reviewed
• [Journal Article] Molecular Mechanism of Immunoglobulin V-Region Diversification Regulated by Transcription and RNA Metabolism in Antigen-Driven B Cells (2011)
  • Author(s): Sakaguchi N, et al
  • Journal Title: Scandinavian Journal of Immunology Volume: 73 Issue: 6 Pages: 520-526
  • DOI: 10.1111/j.1365-3083.2011.02557.x
  • Peer Reviewed
• [Presentation] GANP plays a regulatory role in the choice of DNA repair pathway by dissociating DNA-PKcs in AID-induced immunoglobulin V-region diversification (2014)
  • Author(s): Sakaguchi N, Eid MMA, Singh SK, Almofty SA, Shimoda M, Maeda K
  • Organizer: Keystone Symposia Conference Biology of B Cell Responses
  • Place of Presentation: Keystone Conference Center (コロラド, アメリカ)
  • Year and Date: 2014-02-13
• [Presentation] GANP plays a regulatory role in the choice of DNA repair pathway by dissociating DNA-PKcs in AID-induced immunoglobulin V-region diversification (2014)
  • Author(s): Sakaguchi, N., Eid, M. M. A., Singh, S. K., Almofty, S. A., Shimoda, M, Maeda, K.
  • Organizer: Keystone Symposia Conference Biology of B Cell Responses
  • Place of Presentation: Keystone, Colorado, USA
• [Presentation] GANP regulates transcription and nucleosome occupancy at the immunoglobulin variable region for AID-accession (2013)
  • Author(s): Maeda K, Singh SK, Eid MMA, Almofty SA, Shimoda M, Kawabata T, Sakaguchi N
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2013-12-13
• [Presentation] B cell-associated regulation of DNA repair pathway through interaction with DNA-PKcs in IgV-region diversification (2013)
  • Author(s): Eid MMA, Maeda K, Almofty SA, Singh SK, Shimoda M, Sakaguchi N
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2013-12-13
• [Presentation] Role of mammalian TREX-2 component in HIV-1 mRNA export (2013)
  • Author(s): Almofty, S. A., Maeda, K., Singh, S. K., Eid, M. M. A., Shimoda, M., Kawabata, T., Sakaguchi, N.
  • Organizer: The 42th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 千葉幕張メッセ
• [Presentation] B cell-associated regulation of DNA repair pathway through interaction with DNA-PKcs in IgV-region diversification (2013)
  • Author(s): Eid, M. M. A., Maeda, K., Almofty, S. A., Singh, S. K., Shimoda, M., Sakaguchi, N.
  • Organizer: The 42th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 千葉幕張メッセ
• [Presentation] GANP regulates transcription and nucleosome occupancy at the immunoglobulin variable region for AID-accession (2013)
  • Author(s): Maeda, K., Singh, S. K., Eid, M. M. A., Almofty, S. A., Shimoda, M., Kawabata, T., Sakaguchi, N.
  • Organizer: The 42th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 千葉幕張メッセ
• [Presentation] Protein phosphatase subunit G5PR regulates the plasma blast differentiation and autoantibody production of peritoneal B-1a cells (2013)
  • Author(s): Kitabatake, M., Zhang, T. L., Kuwahara K., Sakaguchi, N.
  • Organizer: The 42th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 千葉幕張メッセ
• [Presentation] Mammalian Leng8 carrying a Sac3/GANP homologous region unregulated in germinal center B cells is involved in regulation of BCR-mediated proliferation (2013)
  • Author(s): Kuwahara, K., Kitabatake, M., Kitamura, D., Sakaguchi, N.
  • Organizer: The 42th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 千葉幕張メッセ
• [Presentation] Role of mammalian TREX-2 component GANP in HIV-1 infection (2013)
  • Author(s): Maeda, K., Almofty, S. A., Shimoda, M., Singh, S. K., Eid, M. M. A., Kawabata, T., Ikeda, T., Shida, H., Koito, A., Sakaguchi, N.
  • Organizer: The 14th Kumamoto AIDS Seminar GCOE Joint International Symposium
  • Place of Presentation: Kumamoto Castle Hotel, Aso Resort Grandvrio Hotel
• [Presentation] HIV感染経路における宿主因子GANPの役割 (2013)
  • Author(s): 前田和彦, アルモフティ・サーラ アミーン, シン・シャイレンドラ クマール, エイド・モハメド アバス, 下田真唯子, 川畑輝弥, 阪口薫雄
  • Organizer: 第27回日本エイズ学会学術集会
  • Place of Presentation: 市民会館崇城大学ホール(熊本市民会館)、熊本市国際交流会館
• [Presentation] 乳癌発症におけるmRNA核外輸送複合体分子GANP異常の関与 (2013)
  • Author(s): 桑原 一彦、阪口 薫雄
  • Organizer: 第17回日本がん分子標的治療学会学術集会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 乳癌発症における TREX2 複合体の役割 (2013)
  • Author(s): 桑原 一彦、岩瀬 弘敬、阪口 薫雄
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] GANP regulates transcription and nucleosome occupancy at the immunoglobulin variable region for AID-accession (2012)
  • Author(s): Maeda K, Singh SK, Almofty SA, Eid MMA, Shimoda M, Sakaguchi N
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場, 神戸国際展示場, 神戸ポートピアホテル(兵庫)
  • Year and Date: 2012-12-05
• [Presentation] Regulation of immunoglobulin variable region diversification through GANP-mediated regulation of DNA double-strand break repair machinery (2012)
  • Author(s): Eid MMA, Maeda K, Singh SK, Almofty SA, Shimoda M, Sakaguchi N
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場, 神戸国際展示場, 神戸ポートピアホテル(兵庫)
  • Year and Date: 2012-12-05
• [Presentation] Targeting the rearranged immunoglobulin V-region locus in B-cells by the histone acetyl transcriptase activity of GANP (2012)
  • Author(s): Maeda K, Singh SK, Almofty SA, Eid MMA, Ono M, Sakaguchi N
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 学術総合センター(東京)
  • Year and Date: 2012-05-14
• [Presentation] Role of GANP in transcriptional regulation of immunoglobulin gene locus (2012)
  • Author(s): Sakaguchi N, Singh SK, Almofty SA, Eid MMA, Ono M, Maeda K
  • Organizer: Keystone Symposia Mutations, Malignancy and Memory-Antibodies and Immunity
  • Place of Presentation: Boston, Boston Park Plaza & Towers (ボストン, アメリカ)
  • Year and Date: 2012-03-21
• [Presentation] GANP associated with HIV-1 RNA export is encapsidated with APOBEC3G into virion core (2012)
  • Author(s): Maeda K
  • Organizer: Keystone Symposia Mutations, Malignancy and Memory-Antibodies and Immunity
  • Place of Presentation: Boston Park Plaza & Towers・アメリカ
  • Year and Date: 2012-03-20
• [Presentation] Role of GANP in transcriptional regulation of immunoglobulin gene locus (2012)
  • Author(s): Sakaguchi N
  • Organizer: Keystone Symposia Mutations, Malignancy and Memory-Antibodies and Immunity
  • Place of Presentation: Boston Park Plaza & Towers・アメリカ
  • Year and Date: 2012-03-20
• [Presentation] Monoclonal antibodies (mAbs) against membrane fusion on HIV envelope-fusion complex (FC) enhanced appearance of neutralization target (2012)
  • Author(s): Toda T
  • Organizer: 新興・再興感染症に関するアジアリサーチフォーラム-2012
  • Place of Presentation: 神戸国際会議場・兵庫
  • Year and Date: 2012-01-10
• [Presentation] Targeting the rearranged immunoglobulin V-region locus in B-cells by the histone acetyl transcriptase activity of GANP (2012)
  • Author(s): Kazuhiko Maeda
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 学術総合センター（東京一ツ橋）
• [Presentation] siGanpによるp53変異悪性腫瘍特異的な腫瘍増殖阻止 (2012)
  • Author(s): 桑原 一彦
  • Organizer: 第16回日本がん分子標的治療学会学術集会
  • Place of Presentation: 北九州市 西日本総合展示場 AIM3F
• [Presentation] 高親和性B細胞制御と抗癌単クローン抗体 (2012)
  • Author(s): 阪口薫雄
  • Organizer: 第16回日本がん免疫学会総会
  • Place of Presentation: 北海道大学 学術交流会館
• [Presentation] Ganp siRNAはp53不活性型悪性腫瘍に選択的に細胞死を誘導することができる (2012)
  • Author(s): 桑原 一彦
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ホテルロイトン札幌
• [Presentation] 乳腺上皮細胞におけるエストロゲンが誘導するDNA損傷においてGANPは重要な役割を果たす (2012)
  • Author(s): 阪口薫雄
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ホテルロイトン札幌
• [Presentation] Mammalian TREX-2 component GANP is involved in HIV-1 mRNA export (2012)
  • Author(s): Kazuhiko Maeda
  • Organizer: The 13th Kumamoto AIDS Seminar GCOE Joint International Symposium
  • Place of Presentation: Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel
• [Presentation] GANPを介したAPOBEC3Gの抗HIV感染阻止機構の解析 (2012)
  • Author(s): 前田和彦
  • Organizer: 第26回日本エイズ学会学術集会・総会
  • Place of Presentation: 慶応義塾大学 日吉キャンパス
• [Presentation] GANP regulates transcription and nucleosome occupancy at the immunoglobulin variable region for AID-accession (2012)
  • Author(s): Kazuhiko Maeda
  • Organizer: The 41th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
• [Presentation] Regulation of immunoglobulin variableregion diversification through GANP-mediated regulation of DNA double-strand break repair machinery (2012)
  • Author(s): Mohammed Mansour Abbas Eid
  • Organizer: The 41th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
• [Presentation] Escorting APOBEC3G toward the fulllength HIV-1 mRNA at the cytoplasmic P bodies (2012)
  • Author(s): Sarah Ameen Almofty
  • Organizer: The 41th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
• [Presentation] GANP regulates APOBEC3G targeting for reduction of HIV-1 infectivity (2012)
  • Author(s): Shailendra Kumar Singh
  • Organizer: The 41th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
• [Presentation] Lyn-mediated signaling to GANP upregulation is essential for persistence of high-affinity antigen-specific B-cells during immune response (2012)
  • Author(s): Kazuhiko Kuwahara
  • Organizer: The 41th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
• [Presentation] Survival of antigen-driven germinal center B-cell is controlled by centrocyte-associated expression of apoptosis-regulator G5PR (2012)
  • Author(s): Masahiro Kitabatake
  • Organizer: The 41th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
• [Presentation] APOBEC3GのHIV-1粒子内への取り込みを制御する宿主因子GANP (2011)
  • Author(s): 前田和彦
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: ハイアットリージェンシー東京
  • Year and Date: 2011-12-01
• [Presentation] HIV-1 gp120/CD4&CXCR4膜融合複合体エピトープと感染阻止効果 (2011)
  • Author(s): 戸田哲平
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: ハイアットリージェンシー東京
  • Year and Date: 2011-11-30
• [Presentation] Analysis of GANP-associated complex in B-cell nuclei (2011)
  • Author(s): Maeda K, Singh SK, Almofty SA, Eid MMA, Sakaguchi N
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-28
• [Presentation] Involvement of GANP in transcriptional regulation of immunoglobulin gene locus (2011)
  • Author(s): Singh SK, Maeda K, Almofty SA, Eid MMA, Sakaguchi N
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-28
• [Presentation] GANP augments the immunoglobulin V region gene conversion via Ku70 dependent mechanism (2011)
  • Author(s): Eid MMA, Maeda K, Singh SK, Almofty SA, Sakaguchi N
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-28
• [Presentation] 広範な中和活性を有する抗体エピトープの同定を目的としたHIV env-CD4膜融合複合体に対するモノクローナル抗体の開発 (2011)
  • Author(s): 戸田哲平
  • Organizer: The 40th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-11-28
• [Presentation] GANP is required for prevention of DNA damage and cell apoptosis (2011)
  • Author(s): Phimsen S
  • Organizer: The 40th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-11-28
• [Presentation] Analysis of GANP-associated complex in B-cell nuclei (2011)
  • Author(s): Maeda K
  • Organizer: The 40th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-11-28
• [Presentation] Involvement of GANP in transcriptional regulation of immunoglobulin gene locus (2011)
  • Author(s): Singh SK
  • Organizer: The 40th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-11-28
• [Presentation] GANP augments the immunoglobulin Vregion gene conversion via Ku70 dependent mechanism (2011)
  • Author(s): Eid MMA
  • Organizer: The 40th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-11-28
• [Presentation] Lynを介するシグナルターゲットであるGANPは末梢リンパ組織における高親和性抗体産生B細胞の維持に重要である (2011)
  • Author(s): 桑原一彦
  • Organizer: The 40th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-11-27
• [Presentation] An apoptotic regulator G5PR is highly expressed in centrocytes and critical for B cell survival in mice (2011)
  • Author(s): Kitabatake M
  • Organizer: The 40th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-11-26
• [Presentation] GANP promotes APOBEC3G encapsidation in virions to reduce viral infectivity (2011)
  • Author(s): Maeda K
  • Organizer: The 12th Kumamoto AIDS Seminar GCOE Joint International Symposium
  • Place of Presentation: Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel
  • Year and Date: 2011-10-21
• [Presentation] Dynamic changes in antigenic memberane fusion epitopes initiated by interactions between HIV-1 envelope proteins and CD4/CXCR4 (2011)
  • Author(s): Toda T
  • Organizer: The 12th Kumamoto AIDS Seminar GCOE Joint International Symposium
  • Place of Presentation: Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel
  • Year and Date: 2011-10-20
• [Presentation] Glycans in MUC5AC mucin as diagnostic/prognostic and early markers for cholangiocarcinoma (2011)
  • Author(s): Wongkham S
  • Organizer: 日本癌学会学術集会
  • Place of Presentation: 名古屋国際会議場・愛知
  • Year and Date: 2011-10-04
• [Presentation] 乳腺特異的GANP欠損マウスにおける乳管形成障害と相関する高頻度乳癌発症 (2011)
  • Author(s): 桑原一彦
  • Organizer: 日本癌学会学術集会
  • Place of Presentation: 名古屋国際会議場・愛知
  • Year and Date: 2011-10-04
• [Presentation] 高親和性単クローン抗体による胆管癌特異的糖鎖エピトープの同定 (2011)
  • Author(s): 阪口薫雄
  • Organizer: 日本がん免疫学会
  • Place of Presentation: 千里ライフサイエンスセンター大阪
  • Year and Date: 2011-07-01
• [Presentation] 乳腺特異的GANP欠損マウスにおける乳癌発症機構の解析 (2011)
  • Author(s): 桑原一彦
  • Organizer: 日本がん分子標的治療学会
  • Place of Presentation: ホテル日航東京
  • Year and Date: 2011-06-24
• [Presentation] RNA metabolism and antibody affinity maturation (2011)
  • Author(s): Sakaguchi N
  • Organizer: Immunology Symposium Basel (ISB) 2011 on "Immune Responses and Autoimmunity"
  • Place of Presentation: University of Basel、スイフ
  • Year and Date: 2011-06-10
• [Presentation] GANP associated with HIV-1 RNA export is encapsidated with Apobec3G into virion cores (2011)
  • Author(s): Maeda K
  • Organizer: The 2011 Meeting on Retroviruses at Cold Spring Harbor Laboratory
  • Place of Presentation: Cold Spring Harbor, New York
  • Year and Date: 2011-05-25
• [Presentation] Transcriptional targeting at immunoglobulin V-region locus by an RNA-export molecule GANP (2011)
  • Author(s): Maeda K, Singh SK, Almofty SA, Eid MAA, Sakaguchi N
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本(熊本)
  • Year and Date: 2011-05-20
• [Presentation] Transcriptional targeting at immunoglobulin V-region locus by an RNA-exportmolecule GANP (2011)
  • Author(s): 前田和彦
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本、熊本
  • Year and Date: 2011-05-20
• [Book] 臨床免疫・アレルギー科, 免疫グロブリンの体細胞高頻度突然変異誘導と GANP (2014)
  • Author(s): 下田真唯子, 前田和彦, 阪口薫雄
  • Publisher: 科学評論社
• [Book] 臨床免疫・アレルギー科, 胚中心B細胞の高親和性成熟における GANP の役割 (2013)
  • Author(s): 前田和彦, 阪口薫雄
  • Publisher: 科学評論社
• [Book] 胚中心B細胞の高親和性成熟におけるGANPの役割 (2013)
  • Author(s): 前田和彦、阪口薫雄
  • Publisher: 臨床免疫・アレルギー科 科学評論社
• [Book] 臨床免疫・アレルギー科, B細胞の生存維持とmiRNA (2012)
  • Author(s): 前田和彦, 阪口薫雄
  • Publisher: 科学評論社
• [Book] 免疫学コア講義改訂3版 (2012)
  • Author(s): 阪口薫雄, 前田和彦
  • Publisher: 南山堂
• [Book] 臨床免疫・アレルギー科, プロテインホスファターゼ 2A の調節と自己免疫 (2012)
  • Author(s): 前田和彦, 阪口薫雄
  • Publisher: 科学評論社
• [Book] B細胞の生存維持とmiRNA (2012)
  • Author(s): 前田和彦
  • Publisher: 科学評論社
• [Book] プロテインホスファターゼ2Aの調節と自己免疫 (2012)
  • Author(s): 前田和彦, 他
  • Publisher: 科学評論社
• [Book] 自己免疫疾患とB1細胞 (2012)
  • Author(s): 北畠正大, 他
  • Publisher: 科学評論社
• [Book] 免疫記憶の制御と疾患/B 細胞免疫記憶:免疫記憶研究のフロンティア, 実験医学, 高親和性抗体産生のメカニズム (2011)
  • Author(s): 前田和彦, 阪口薫雄
  • Publisher: 羊土社
• [Book] 臨床免疫・アレルギー科, 胚中心B細胞の分化・増殖と GANP (2011)
  • Author(s): 前田和彦, 阪口薫雄
  • Publisher: 科学評論社
• [Book] 高親和性抗体産生のメカニズム (2011)
  • Author(s): 前田和彦, 他
  • Publisher: 羊土社
• [Book] 胚中心B細胞の分化・増殖とGANP (2011)
  • Author(s): 前田和彦, 他
  • Publisher: 科学評論社
• [Remarks] 新聞報道2013年5月15日, 熊本日日新聞, 「遺伝子変異させ強力な抗体」
  • URL: http://www.k-immu.jp/ja/
• [Remarks]
  • URL: http://www.k-immu.jp/ja/index.html