Diagnostic methods to predict the development of autoimmune diseases by gene polymorphisms of immune regulatory factors
Project/Area Number |
23390150
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Osaka University |
Principal Investigator |
YOSHINORI Iwatani 大阪大学, 医学(系)研究科(研究院), 教授 (60168581)
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Co-Investigator(Kenkyū-buntansha) |
HIDAKA Yoh 大阪大学, 大学院・医学系研究科, 准教授 (30243231)
WATANABE Mikio 大阪大学, 医学(系)研究科(研究院), 准教授 (50294088)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
Keywords | 免疫応答制御因子 / 遺伝子多型 / 自己免疫疾患 / 発症予知診断法 / 疾患感受性遺伝子 / サイトカイン / DNAメチル化酵素 / CD24 / IL-10 / EPO / 一塩基多型 / 発症予知 / 重症度 / CD40 / FCRL3 / DNMT1 / MTRR / マスター遺伝子 / Th1細胞 / Th2細胞 |
Research Abstract |
In order to develop the predictive diagnostic methods for the development and prognosis of autoimmune thyroid diseases, we analyzed the single mononucleotide polymorphisms (SNPs) of immune regulatory factors in patients with Hashimoto's disease and with Graves' disease. We clarified the usefulness of ZFAT Ex9b-SNP10T/A, MTRR +66A/G, IL-10 -592A/C and TSHR rs179247A/G SNPs to predict the development of hypothyroidism under 50 years old in patients with Hashimoto's disease, and that of TBX21 -1514T/C, HLX -742C/G, IL-13 -1112C/T, CD40 -1C/T,FCRL3 -169C/T, DNMT1 +32204A/G and TSHR rs179247A/G SNPs to predict the intractability of Graves' disease against anti-thyroid drug therapy. Furthermore, we clarified the importance of an EPO -1125G/T SNP which determine the strength of angiogenic capability for the development of Graves' disease. These data indicate that the SNPs of immune regulatory factors are useful for the predictive diagnostic methods for autoimmune diseases.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] IL-10 -592A/C polymorphism is associated with severity of Hashimoto's disease2013
Author(s)
Inoue, N., Watanabe, M., Wada, M., Morita, M., Hidaka, Y., Iwatani, Y
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Journal Title
Cytokine
Volume: 64
Issue: 1
Pages: 370-374
DOI
Related Report
Peer Reviewed
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[Journal Article] Preparation of thyroid follicular cells for mRNA quantifi cation after fl uorescence-activated cell sorting2013
Author(s)
Matsumoto, C., Ito, M., Yamada, H., Yoshida, H., Watanabe, M., Hidaka, Y., Iwatani, Y., Miyauchi, A., Takano, T
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Journal Title
Scandinavian Journal of Clinical & Laboratory Investigation
Volume: 73
Issue: 3
Pages: 245-252
DOI
Related Report
Peer Reviewed
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[Journal Article] Genes that characterize T3-predominant Graves' thyroid tissues2013
Author(s)
Matsumoto, C., Ito, M., Yamada, H., Yamakawa, N., Yoshida, H., Date, A., Watanabe, M., Hidaka, Y., Iwatani, Y., Miyauchi, A., Takano, T
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Journal Title
Eur J Endocrinol
Volume: 168(2)
Issue: 2
Pages: 137-44
DOI
Related Report
Peer Reviewed
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[Journal Article] Association ofpolymorphisms in DNMT1, DNMT3A, DNMT3B, MTHFR, and MTRR genes with global DNA methylation levels and prognosis of autoimmune thyroid disease2012
Author(s)
Arakawa, Y., Watanabe, M., Inoue, N., Sarumaru, M., Hidaka, Y., Iwatani, Y
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Journal Title
Clin Exp Immunol
Volume: 170(2)
Issue: 2
Pages: 194-201
DOI
Related Report
Peer Reviewed
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[Journal Article] Associations between autoimmune thyroid disease prognosis and functional polymorphisms of susceptibility genes, CTLA4, PTPN22, CD40, FCRL3, and ZFAT, previously revealed in Genome-wide association studies2012
Author(s)
Inoue, N. Watanabe, M., Yamada, H., Takemura, K., Hayashi, F., Yamakawa, N., Akahane, M., Shimizuishi, Y., Hidaka, Y., Iwatani, Y
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Journal Title
J Clin Immunol
Volume: 32
Issue: 6
Pages: 1243-52
DOI
Related Report
Peer Reviewed
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[Journal Article] Measurement of <i>TFF3</i> mRNA in aspirates from thyroid nodules using mesh filtration: The first clinical trial in 130 cases2012
Author(s)
Yamada, H., Takano, T., Kihara, M., Hirokawa, M., Yoshida, H., Watanabe, M., Iwatani, Y., Hidaka, Y., Miyauchi, A
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Journal Title
Endocrine Journal
Volume: 59
Issue: 7
Pages: 621-630
DOI
NAID
ISSN
0918-8959, 1348-4540
Related Report
Peer Reviewed
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[Journal Article] Prolonged hybridization with a cRNA probe improves the signal to noise ratio for in-tube in situ hybridization for quantification of mRNA after fluorescence-activated cell sorting2012
Author(s)
Yamada, H., Yamakawa, N., Watanabe, M., Hidaka, Y., Iwatani, Y., Takano, T.
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Journal Title
Biotech Histochem
Volume: (in press)
Issue: 5
Pages: 366-71
DOI
Related Report
Peer Reviewed
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[Journal Article] Preparation of a cut-off solution that gives a clear index for distinguishing Le(a-b-) sera in CA19-9 assays using SphereLight 180 and Architect i2000 kits2012
Author(s)
Hanada, H., Mugii, S., Takeoka, K., Maeda, I., Watanabe, M., Hidaka, Y., Iwatani, Y
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Journal Title
Clinica Chemica Acta
Volume: 413(1-2)
Pages: 278-281
Related Report
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[Journal Article] Functional polymorphisms in TBX21 and HLX are associated with development and prognosis of Graves' disease2011
Author(s)
Morita, M., Watanabe, M., Inoue, N., Inaoka, C., Akamizu, T., Tatsumi, K., Hidaka, Y., Iwatani, Y.
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Journal Title
Autoimmunity
Volume: 45(2)
Issue: 2
Pages: 129-36
DOI
Related Report
Peer Reviewed
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[Journal Article] Association of functional GITR gene polymorphisms related to expression of GITR molecules with prognosis of autoimmune thyroid disease2011
Author(s)
Tomizawa, R., Watanabe, M., Inoue N., Takemura, K., Hidaka, Y., Akamizu, T., Hayakawa, K., Iwatani, Y
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Journal Title
Clin Exp Immunol
Volume: 165(2)
Pages: 141-147
Related Report
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[Journal Article] Association of functional polymorphisms in promoter regions of IL5, IL6, and IL13 genes with development and prognosis of autoimmune thyroid diseases2011
Author(s)
Inoue, N., Watanabe, M., Morita, M., Tatsumi, K., Hidaka, Y., Akamizu, T., Iwatani, Y
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Journal Title
Clin Exp Immunol
Volume: 163(3)
Pages: 318-323
Related Report
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[Journal Article] mRNA quantification after fluorescence activated cell sorting using locked nucleic acid probes2011
Author(s)
Maruo, R., Yamada, H., Watanabe, M., Hidaka, Y., Iwatani, Y. Takano, T
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Journal Title
Mol Biotechnol
Volume: 49(1)
Pages: 42-47
Related Report
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[Journal Article] Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines2011
Author(s)
Yamada, H., Takano, T., Matsuzuka, F., Watanabe, M., Miyauchi, A., Iwatani, Y
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Journal Title
Genet Mol Biol
Volume: 34(1)
Pages: 6-10
Related Report
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[Journal Article] Association of functional polymorphisms in promoter regions of IL5, IL6, and IL13 genes with development and prognosis of autoimmune thyroid diseases2011
Author(s)
Inoue, N., Watanabe, M., Morita, M., Tatsumi, K., Hidaka, Y., Akamizu, T., Iwatani, Y.
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Journal Title
ClinExp Immunol
Volume: 163(3)
Issue: 3
Pages: 318-23
DOI
Related Report
Peer Reviewed
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[Journal Article] Association of functional GITR gene polymorphisms related to expression of GITR molecules with prognosis of autoimmune thyroid disease2011
Author(s)
Tomizawa, R., Watanabe, M., Inoue N., Takemura, K., Hidaka, Y., Akamizu, T., Hayakawa, K., Iwatani, Y.
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Journal Title
Clin Exp Immunol
Volume: 165(2)
Issue: 2
Pages: 141-147
DOI
Related Report
Peer Reviewed
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[Journal Article] mRNA quantification after fluorescence activated cell sorting using locked nucleic acid probes2011
Author(s)
Maruo, R., Yamada, H., Watanabe, M., Hidaka, Y., Iwatani, Y. Takano, T.
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Journal Title
Mol Biotechnol
Volume: 49(1)
Issue: 1
Pages: 42-47
DOI
Related Report
Peer Reviewed
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[Journal Article] Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines2011
Author(s)
Yamada, H., Takano, T., Matsuzuka, F., Watanabe, M., Miyauchi, A., Iwatani, Y.
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Journal Title
Genet Mol Biol
Volume: 34(1)
Issue: 1
Pages: 6-10
DOI
Related Report
Peer Reviewed
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