| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Outline of Final Research Achievements |
RNA aptamers are artificial synthetic ribonucleic acids that form a higher-order structure and bind phosphorylated sites of specific protein. We will develop novel cancer therapy by utilizing RNA aptamers for controlling radio-sensitivities of malignant cells during radiation therapy for cancer. We tried making RNA aptamers against phosphorylated sites of DNA-PKcs by SELEX, and produced specific molecules to DNA-PK. Some of the RNA enhanced sensitivity of radiation for U2OS cells. In this experiment, we also try to construct an expression system of RNA aptamers using lenti-virus RNA expression vector, and try to find a good candidate molecule for therapy. Furthermore, for developing better radiation therapy, we combine RNA technology and live cell-imaging technology for evaluating DNA damage and cell cycle progression.
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