| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Research Abstract |
This study examined to clarify the heat sensitivity in X-ray resistant cells from human tongue cancer cells. Our results show that heat sensitivity was not significant difference between X-ray resistant cells and parental cells. The data of flow cytometory showed that cancer stem-like cells were significantly decreased after heating as compared with exposure to X-rays at iso-survival doses in both cells.
In addition, we examined the effect of the inhibitor of HR repair or NHEJ repair for DSBs on heat sensitivity. Heat sensitivity was not affected by NHEJ inhibitor. On the other hand, HR inhibitor was able to enhance heat sensitivity. These results suggest that the heat sensitivity may be enhanced by the suppression of HR repair. In addition, these findings provide support for the concept that heat may lead to the induction of DSBs.
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