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Development of bioluminescent probes to visualize protein activity involved in cell signaling

Research Project

Project/Area Number 23390317
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

MORITA NAOKI  独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (60371085)

Co-Investigator(Kenkyū-buntansha) HAGA Sanae  北海道大学, 大学院・保健科学研究院, 博士研究員 (60706505)
OZAKI Michitaka  北海道大学, 大学院・保健科学研究院, 教授 (80256510)
Co-Investigator(Renkei-kenkyūsha) OHMIYA Yoshihiro  独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究部門長 (20223951)
OZAWA Takeaki  東京大学, 大学院・理学系研究科, 教授 (40302806)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords生体イメージング / 発光プローブ / 細胞情報伝達 / 創薬 / 抗癌剤
Research Abstract

In this study, we developed molecular probes for imaging the activation of Akt (Akt probe) and endoplasmic reticulum (ER) stress (CHOP and PERK probe) by using the "luciferase reconstitution system" technique. For Akt probe, we tried to build bioluminescent probes that emits light when stimulus enters (= when Akt is activated). It is known that PERK molecules dimerize when cells sense ER stress. Therefore, we also constructed bioluminescent PERK probes that detect ER stress by monitoring dimerization of PERK molecules using the "luciferase reconstitution system" technique.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (21 results)

All 2014 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 1 results) Presentation (16 results)

  • [Journal Article] Bio-imaging of surgical stress : Dynamic analysis of liver oxidative stress and damage2012

    • Author(s)
      Naoki Morita, Sanae Haga, Takeaki Ozawa, James S. Remington, and Michitaka Ozaki
    • Journal Title

      Luminescence

      Volume: 27 Pages: 143-145

    • Related Report
      2013 Final Research Report
  • [Journal Article] Bio-imaging of surgical stresses - Dynamic analyses of liver oxidative stress and damage -2012

    • Author(s)
      Michitaka Ozaki, Sanae Haga, Takeaki Ozawa, Naoki Morita, Yoshimi Kaneshima, and James S. Remington
    • Journal Title

      5th European Conference of the International Federation for Medical and Biological Engineering (IFMBE) Proceedings

      Volume: Volume37,Part1,Part10 Pages: 1094-1097

    • Related Report
      2013 Final Research Report
  • [Journal Article] 'Collagen vitrigel sheet' as a novel drug delivery bio-material2012

    • Author(s)
      Sanae Haga, Toshiaki Takezawa, Takeaki Ozawa, James. S. Remington, Naoki Morita and Michitaka Ozaki
    • Journal Title

      5th European Conference of the International Federation for Medical and Biological Engineering (IFMBE) Proceedings

      Volume: Volume37,Part1,Part14 Pages: 1354-1357

    • Related Report
      2013 Final Research Report
  • [Journal Article] Bio-imaging of surgical stress: Dynamic analysis of liver oxidative stress and damage.2012

    • Author(s)
      Naoki Morita
    • Journal Title

      Luminescence

      Volume: 27 Pages: 143-145

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 移植臓器の非侵襲的モニタリング法の開発2011

    • Author(s)
      尾崎 倫孝, 芳賀 早苗, 森田 直樹, 深井 原, 藤堂 省, 小澤 岳昌, 近江谷 克裕
    • Journal Title

      Organ Biology

      Volume: 18 Pages: 134-140

    • NAID

      130004709996

    • Related Report
      2013 Final Research Report
  • [Presentation] 新規分泌型発光プローブをもちいたマウス腫瘍の増殖モニタリング2014

    • Author(s)
      森田直樹、尾崎倫孝
    • Organizer
      第18回日本がん分子標的治療学会学術集会
    • Place of Presentation
      仙台市情報・産業プラザ
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光学技術を応用した新たな臓器保存法, 細胞・臓器移植法の開発2013

    • Author(s)
      尾崎 倫孝, 芳賀 早苗, 森田 直樹, 野田 なつみ, 山田 勇磨, 小澤 岳昌
    • Organizer
      第40回日本臓器保存生物医学会学術集会
    • Place of Presentation
      東京医科大学病院臨床講堂
    • Related Report
      2013 Final Research Report
  • [Presentation] p62/SQSTM1 plays a protective role from oxidative injury of steatotic liver in mouse hepatectomy model2013

    • Author(s)
      Sanae Haga, Takeaki Ozawa, Naoki Morita, Yayoi Seki, Yuma Yamada, Yukari Yasuzaki, Kazuo Umezawa, and Michitaka Ozaki
    • Organizer
      The 20th Annual Meeting of the Japanese Society for the Research of Hepatic Cells
    • Place of Presentation
      Osaka International Convention Center, Osaka
    • Related Report
      2013 Final Research Report
  • [Presentation] 分泌型ルシフェラーゼを用いた腫瘍細胞進展のモニタリング2013

    • Author(s)
      森田 直樹, 芳賀 早苗, 関 弥生, 樋口 美保, 呉 純, 尾崎 倫孝, 近江谷 克裕
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] p62/STSQM1 plays a central role in liver injury in steatotic liver during regeneration2012

    • Author(s)
      芳賀 早苗, 小澤 岳昌, 森田 直樹, 梅澤 一夫, 藤堂 省, 尾崎 倫孝
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 虚 血/再灌流傷害における高血糖および脂肪化病態肝の反応性の相違2012

    • Author(s)
      芳賀 早苗, 小澤 岳昌, 森田 直樹, 阿部 理一郎, 藤堂 省, 尾崎 倫孝
    • Organizer
      第19回肝細胞研究会
    • Place of Presentation
      札幌医科大学臨床教育研究棟講堂
    • Related Report
      2013 Final Research Report
  • [Presentation] Bio-imaging of surgical stress : Dynamic analysis of liver oxidative stress and damage2012

    • Author(s)
      Naoki Morita, Sanae Haga, Takeaki Ozawa, James S. Remington, and Michitaka Ozaki
    • Organizer
      17th International Symposium on Bioluminescence and Chemiluminescence (ISBC2012)
    • Place of Presentation
      Delta Guelph Hotel & Conference Centre, Guelph, Ontario, Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] Bio-imaging of surgical stress: Dynamic analysis of liver oxidative stress and damage.2012

    • Author(s)
      Naoki Morita
    • Organizer
      17th International Symposium on Bioluminescence and Chemiluminescence (ISBC2012)
    • Place of Presentation
      Guelph, Ontario, Canada
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of different cellular responses of cells in hyperglycemic condition or steatotic hepatocytes - a simulated study of stress response in metabolic syndrome -2011

    • Author(s)
      Delta Guelph Hotel & Conference Centre, Guelph, Ontario, Canada
    • Organizer
      第34回日本分子生物学会年会(MBSJ2011)
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] Age-associated p66Shc induced redox-dependent liver damage and impaired regeneration after hepatectomy in aged mice2011

    • Author(s)
      Michitaka Ozaki, Sanae Haga, Kaikobad Irani, Naoki Morita, Yoshimi Kaneshima, and Takeaki Ozawa
    • Organizer
      62th Annual meeting of AASLD
    • Place of Presentation
      Moscone West Convention Center, San Francisco, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Bio-imaging of surgical stresses-dynamic analyses of liver oxidative stress and damage2011

    • Author(s)
      Michitaka Ozaki, Sanae Haga, Takeaki Ozawa, Naoki Morita, Yoshimi Kaneshima, and James S. Remington
    • Organizer
      5th European Conference of the International Federation for Medical and Biological Engineering
    • Place of Presentation
      Budapest Congress & World Trade Center, Budapest, Hungary
    • Related Report
      2013 Final Research Report
  • [Presentation] 脂肪肝におけるFas過剰発現および細胞内酸化的ストレスによる肝再生不全の検討2011

    • Author(s)
      芳賀 早苗, 森田 直樹, 青山 貴子, 小澤 岳昌, James S. Remington, 阿 部 理一郎, 尾崎 倫孝
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Final Research Report
  • [Presentation] 'Collagen vitrigel sheet' as a novel drug delivery bio-material2011

    • Author(s)
      Sanae Haga, Toshiaki Takezawa, Takeaki Ozawa, James S. Remington, Naoki Morita and Michitaka Ozaki
    • Organizer
      5th European Conference of the International Federation for Medical and Biological Engineering
    • Place of Presentation
      Budapest Congress & World Trade Center, Budapest, Hungary
    • Related Report
      2013 Final Research Report
  • [Presentation] 種々の病態における肝再生の分子制御機構の解析(細胞増 殖・細胞成長・細胞死の観点から)2011

    • Author(s)
      尾崎 倫孝, 芳賀 早苗, 森田直樹, 金島由実, James S. Remington, 小澤 岳昌, 井上 啓, 松本 道宏
    • Organizer
      第18回肝細胞研究会
    • Place of Presentation
      東京ガーデンパレス
    • Related Report
      2013 Final Research Report
  • [Presentation] 外科治療による肝細胞スト レスと障害・再生への影響(生体イメージング法をもちいた動的解析)2011

    • Author(s)
      尾崎 倫孝, 芳賀 早苗, 森田 直樹, 青山 貴子, 金島 由実, Kaikobad Irani, James S. Remington
    • Organizer
      第18回細胞研究会
    • Place of Presentation
      東京ガーデンパレス
    • Related Report
      2013 Final Research Report
  • [Presentation] 脂肪肝における肝再生不全機序の解析2011

    • Author(s)
      芳賀 早苗, 森田 直樹, 小澤 岳昌, James S. Remington, 尾崎 倫孝
    • Organizer
      第18回肝細胞研究会
    • Place of Presentation
      東京ガーデンパレス
    • Related Report
      2013 Final Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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