| Project/Area Number |
23390317
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
MORITA NAOKI 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (60371085)
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| Co-Investigator(Kenkyū-buntansha) |
HAGA Sanae 北海道大学, 大学院・保健科学研究院, 博士研究員 (60706505)
OZAKI Michitaka 北海道大学, 大学院・保健科学研究院, 教授 (80256510)
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| Co-Investigator(Renkei-kenkyūsha) |
OHMIYA Yoshihiro 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究部門長 (20223951)
OZAWA Takeaki 東京大学, 大学院・理学系研究科, 教授 (40302806)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | 生体イメージング / 発光プローブ / 細胞情報伝達 / 創薬 / 抗癌剤 |
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| Research Abstract |
In this study, we developed molecular probes for imaging the activation of Akt (Akt probe) and endoplasmic reticulum (ER) stress (CHOP and PERK probe) by using the "luciferase reconstitution system" technique. For Akt probe, we tried to build bioluminescent probes that emits light when stimulus enters (= when Akt is activated). It is known that PERK molecules dimerize when cells sense ER stress. Therefore, we also constructed bioluminescent PERK probes that detect ER stress by monitoring dimerization of PERK molecules using the "luciferase reconstitution system" technique.
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