Roles of new ion channel involved in the regeneration/development of the periodontal Ruffini endings

• Project/Area Number: 23390418
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Niigata University
• Principal Investigator: MAEDA Takeyasu 新潟大学, 医歯学系, 教授 (40183941)
• Co-Investigator(Kenkyū-buntansha): IZUMI Kenji 新潟大学, 医歯学系, 教授 (80242436) ; INOUE Kayoko (NOZAWA Kayoko) 新潟大学, 医歯学系, 特任准教授 (90303130) ; KAWANO Yoshiro 新潟大学, 医歯学系, 助教 (60303129)
• Research Collaborator: OHZAMA Atsushi 新潟大学, 医歯学系, 准教授 (40266169) ; SUZUKI Akiko 新潟大学, 医歯学系, 特任助教 ; HARADA Fumiko 新潟大学, 医歯学系, 科研費雇用研究員 ; YOSHII Tomoko 新潟大学, 大学院・医歯学総合研究科, 大学院生(現研究員) ; IHYO Chika 新潟大学, 大学院・医歯学総合研究科, 大学院生(現教務補佐員)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 歯根膜 / 機械受容器 / ルフィニ神経終末 / ASIC3 / イオンチャネル / 再生 / ASIC / 免疫細胞化学 / 三叉神経節 / 定量解析

Research Abstract

This study examined the regeneration process of the periodontal Ruffini ending, an essential mecahnoreceptor, based on the changes in the expression pattern of acid-sensing ion channel-3 (ASIC3) at the periodontal ligament and trigeminal ganglion. The findings obtained from the nerve injury model (resection of the inferior alveoar nerve at one side) indicate that this molecule is not direcly involved in the axonal regeneration of the periodontal Ruffini endings but rather in neuron-glial interactions such as control of neuronal activity in the trigeminal ganglion.

Research Products

• [Journal Article] Immunoexpression of aquaporin-1 in the rat periodontal ligament during experimental tooth movement (2012)
  • Author(s): T. Yoshii, F. Harada, I Saito, K. Nozawa-Inoue, Y. Kawano, T. Maeda
  • Journal Title: Biomedical Research Volume: 33(4) Pages: 225-233
  • NAID: 130004470825
  • URL: https://www.jstage.jst.go.jp/article/biomedres/33/4/33_225/_article
  • Peer Reviewed
• [Journal Article] e injury provides an essential delivery tool for neuropathic pain attenuation (2012)
  • Author(s): H.M. Zakir, R.M. Mostafeezur, A. Suzuki, S. Hitomi, T. Maeda, K. Seo, Y. Yamada, K. Yamamura, S. Lev, A.M. Binshtok, K. Iwata, J. Kitagawa
  • Journal Title: PLoS One Volume: 7(9) Issue: 9 Pages: e44023-e44023
  • DOI: 10.1371/journal.pone.0044023
  • Peer Reviewed
• [Journal Article] Immunoexpression of aquaporin-1 in the rat periodontal ligament during experimental tooth movement. (2012)
  • Author(s): Yoshii T, Harada F, Saito I, Nozawa-Inoue K, Kawano Y, Maeda T.
  • Journal Title: Biomedical Research Volume: 33(4) Pages: 225-233
  • NAID: 130004470825
  • Peer Reviewed
• [Presentation] ラット切歯歯根膜ルフィニ神経終末の再生過程におけるASIC3 の役割 (2013)
  • Author(s): 井表千馨, 原田史子, 斎藤功, 前田健康
  • Organizer: 平成25年度新潟歯学会第2回例会
  • Place of Presentation: 新潟
  • Year and Date: 2013-11-19
• [Presentation] Possible role of BDNF on nerve regeneration following transection of inferior alveolar nerve (2013)
  • Author(s): Y.M. Valverde Guevara, H. Yoshikawa, I. Saito, T. Maeda, K. Seo
  • Organizer: 平成25年度新潟歯学会総会
  • Place of Presentation: 新潟
  • Year and Date: 2013-04-20
• [Presentation] Contribution of BDNF to nerve regeneration and neuroma formation after nerve injury (2013)
  • Author(s): K. Seo, H. Yoshikawa, Y.M. Valverde Guevara, T. Maeda, M. Kurose, K. Yamamura
  • Organizer: 8th Congress of the European Federation of IASP
  • Place of Presentation: Florence, Italy
• [Presentation] Ruffini ending as a primary mechanoreceptor in periodontal ligament ; Its morphology, histochemical features and neuroplasticity- (2013)
  • Author(s): T. Maeda
  • Organizer: KPPIKG 2013
  • Place of Presentation: KPPIKG 2013(
  • Invited
• [Presentation] Contribution of BDNF to nerve regeneration and neuroma formation after nerve injury (2013)
  • Author(s): Seo K, Yoshikawa H, Valverde YM, Maeda T, Kurose M, Yamamura K
  • Organizer: 8th Congress of the European Federation of IASP
  • Place of Presentation: フィレンツェ、イタリア
• [Presentation] Ruffini ending as a primary mechanoreceptor in periodontal ligament. – Its morphology, histochemical features and neuroplasticity - (2013)
  • Author(s): Maeda T
  • Organizer: KPPIKG 2013
  • Place of Presentation: ジャカルタ、インドネシア
  • Invited
• [Presentation] Effects of antibody to BDNF on nerve regeneration following transection of inferior alveolar nerve (2012)
  • Author(s): Y.M. Valverde Guevara, H. Yoshikawa, K. Seo, T. Maeda, I. Saito
  • Organizer: 42nd Annual Meeting of the Society for Neuroscience
  • Place of Presentation: New Orleans, USA
• [Presentation] 実験的歯の移動時におけるラット臼歯歯根膜のアクアポリン-1免疫発現 (2012)
  • Author(s): 吉居朋子, 斎藤功, 前田健康, 原田史子, 河野芳朗
  • Organizer: 第71回日本矯正歯科学会
  • Place of Presentation: 盛岡
• [Presentation] 実験的歯の移動時におけるラット臼歯歯根膜のアクアポリン-1免疫発現 (2012)
  • Author(s): 吉居朋子，齋藤 功，前田健康，原田史子，河野芳朗
  • Organizer: 第71回日本矯正歯科学会大会
  • Place of Presentation: 盛岡市民文化ホール、盛岡
• [Presentation] Effects of antibody to BDNF on nerve regeneration following transaction of inferior alveolar nerve. (2012)
  • Author(s): Valverde Guevara YM, Yoshikawa H, Seo K, Maeda T, Saito I
  • Organizer: 42nd Annual Meeting of the Society for Neuroscience
  • Place of Presentation: ニューオーリンズ、アメリカ
• [Presentation] Dental Innervation (2011)
  • Author(s): T. Maeda
  • Organizer: Congreso Magno De Egresados De Vuelta A Casa
  • Place of Presentation: Torreon, Mexico
  • Year and Date: 2011-09-16
• [Presentation] Dental innervation (2011)
  • Author(s): T.Maeda
  • Organizer: Congreso Magno De Egresados De Vuelta A Casa
  • Place of Presentation: コアウイラ自治大学,Torreon, Mexico(招待講演)
  • Year and Date: 2011-09-16
• [Presentation] Mechanoreceptive Ruffini endings innervating by ASIC3- positive trigeminal ganglion neurons (2011)
  • Author(s): T. Maeda, et al
  • Organizer: 8th International Brain Research Organization World Congress of Neuroscience
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-07-17
• [Presentation] Mechanoreceptive Ruffini endings innervating by ASIC3-positive trigeminal ganglion neurons (2011)
  • Author(s): T.Maeda, et al.
  • Organizer: 8th International Brain Research Organization World Congress of Neuroscience
  • Place of Presentation: フローレンスコンベンションセンター,Florence, Italy
  • Year and Date: 2011-07-17
• [Presentation] Possible role of BDNF during nerve regeneration after inferior alveolar nerve injury
  • Author(s): Valverde YM, Yoshikawa H, Saito I, Maeda T, Seo K
  • Organizer: 平成25年度新潟歯学会総会
  • Place of Presentation: 新潟
• [Presentation] ラット切歯歯根膜ルフィニ神経終末の再生過程におけるASIC3の役割
  • Author(s): 井表千馨，原田史子，齋藤 功，前田健康
  • Organizer: 平成25年度新潟歯学会第２回例会
  • Place of Presentation: 新潟
• [Book] 第6章 歯周組織の神経支配(口腔組織発生学第2版) (2015)
  • Author(s): 前田健康
  • Publisher: 医歯薬出版(印刷中)
• [Book] 口腔組織発生学第2版（第6章歯周組織の神経支配） (2014)
  • Author(s): 前田健康
  • Total Pages: 400
  • Publisher: 医歯薬出版
• [Book] 口腔の構造と機能(口腔科学) (2013)
  • Author(s): 前田健康
  • Publisher: 朝倉書店
• [Book] 歯の構造と機能(口腔科学) (2013)
  • Author(s): 前田健康
  • Publisher: 朝倉書店
• [Book] 歯根膜の感覚機能(歯と歯列を守る歯根膜活用術) (2011)
  • Author(s): 前田健康
  • Publisher: 医歯薬出版
• [Book] 歯と歯列を守る歯根膜活用術 (2011)
  • Author(s): 前田健康
  • Total Pages: 148
  • Publisher: 医歯薬出版