Production and Evaluation of artificial oral tossue stem cells applied cell reprogramming

• Project/Area Number: 23390470
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: FUKUMOTO Emiko 東北大学, 大学病院, 助教 (10264251)
• Co-Investigator(Kenkyū-buntansha): SAKAI Takayoshi 大阪大学, 大学院・歯学研究科, 教授 (90379082) ; YAMADA Aya 東北大学, 大学院・歯学研究科, 準教授 (40295085)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: 幹細胞 / 口腔組織 / 歯髄 / 分化誘導

Research Abstract

The aim of this research is the application of multipotent stem cells artificially induced from tissue cells to regenerative medicine.
We revealed that GSK3b inhibitor IX induced tooth pulp cells to pulp stem cells, and mediated marker of stem cell. As a result of the transplantation of induced pulp stem cell derived from human deciduous teeth to subcutaneous of mouse using hydroxyapatite as a carrier, hydroxyapatite was absorbed completely and produced substantial bone tissue included human derived blood cells. These results are the innovative discovery that artificial induction of tissue stem cells generated human bone marrow, and the method is expected as the applicable technique for the treatment of blood disorder such as leukemia.

Research Products

• [Journal Article] Establishment of ex vivo mucocele model using salivaly gland organ culture (2014)
  • Author(s): Hino R, Furagi M, Yamada A, Arakaki M, Saito K, Sugawara Y, Ono M, Fukumoto E, Nakamura T, Fukumoto S
  • Journal Title: Pediatric Dental Journal Volume: (in press)
  • Peer Reviewed
• [Journal Article] New insight into the functions of enamel matrices in calcified tissues (2014)
  • Author(s): Fukumoto S, Nakamura T, Yamada A, Arakaki M, Saito K, Xu J, Fukumoto E, Yamada Y
  • Journal Title: Japanease Dental Science Review Volume: (in press)
  • NAID: 50009117196
  • Peer Reviewed
• [Journal Article] Establishment of ex vivo mucocele model using salivary gland organ culture (2014)
  • Author(s): Nino R, Furagi M, Yamada A, Arakaki M, Saito K, Sugawara Y, Ono M, Fukumoto E, Nakamura T, Fukumoto S.
  • Journal Title: Pediatric Dental Journal Volume: in press
  • Peer Reviewed
• [Journal Article] New insights into the functions of enamel matrices in calcified tissues Japanese (2014)
  • Author(s): Fukumoto S, Nakamura T, Yamada A, Arakaki M, Saito K, Xu J, Fukumoto E, Yamada Y.
  • Journal Title: Dental Science Review Volume: in press
  • Peer Reviewed
• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: J Biol Chem Volume: 287(13) Issue: 13 Pages: 10590-601
  • DOI: 10.1074/jbc.m111.285874
  • Peer Reviewed
• [Journal Article] Role of Epithelial-Stem Cell Interactions during Dental Cell Differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: J Biol Chem Volume: 287 Pages: 10590-10601
  • Peer Reviewed
• [Journal Article] IL-12- and IL-18-mediated, nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis of bone marrow cells (2011)
  • Author(s): Kitaura H, Fujimura Y, Yoshimatsu M, Kohara H, Morita Y, Aonuma T, Fukumoto E, Masuyama R, Yoshida N, Takano-Yamamoto T
  • Journal Title: Calcif Tissue Int Volume: 89 Issue: 1 Pages: 65-73
  • DOI: 10.1007/s00223-011-9494-0
  • Peer Reviewed
• [Presentation] 新規エナメル質コート材(PRG バリアコート)のイオン徐放性による再石灰化促進について (2011)
  • Author(s): 菅原優, 山田亜矢, 福本恵美子, 相澤志津子, 岩本勉, 福本敏
  • Organizer: 第49回日本小児歯科学会
  • Place of Presentation: いわて県民情報交流センターアイーナ 盛岡市
  • Year and Date: 2011-11-29
• [Presentation] NF-kB 経路を介した歯の形態形成分子制御機構 (2011)
  • Author(s): 福本敏, 岩本勉, 福島秀文, 山田亜矢, 新垣真紀子, 福本恵美子, 中村卓史, 自見英治郎
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館 京都市
  • Year and Date: 2011-09-23