Establishing revolutional regenerative medicine with universality of social medicine using human dental pulp cells
| Project/Area Number |
23390485
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
YAEGAKI Ken 日本歯科大学, 生命歯学部, 教授 (40166468)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAHARA Taka 日本歯科大学, 生命歯学部, 教授 (10366768)
ISHIKAWA Hiroshi 日本歯科大学, 生命歯学部, 教授 (30089784)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | dental pulp / stem cell / regenerative medicine / liver / pancreas / differentiation / liver cirrhosis / 膵臓 / 再生医療 / 歯髄幹細胞 / 移植 / 肝臓 / 歯髄 / 間葉系幹細胞 / 体性幹細胞 / 無血清培地 / ヒト / 硫化水素 |
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| Outline of Final Research Achievements |
CD117 fraction, which was separated from the primary culture from human tooth pulp cells, was employed for this research. The α-、β-、γ- and δ-cellswere obtained after pancreatic differentiation. After hepatic differentiation all hepatic markers were positive in the most of cells, moreover glycogen accumulation and urea production, which are specific function in hepatic cells, were found.
We developed a novel serum-free media for hepatic differentiation. After implantation of the cells differentiated in the serum-free into immunosuppressive rats, we found successful implantation, proliferation and the formation of blood vessels. Moreover we produced biliary liver cirrhosis in nude rats and transplanted the cells, and then the condition was dramatically improved. No cirrhosis was found.
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Report
(5 results)
Research Products
(9 results)
