| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
CD117 fraction, which was separated from the primary culture from human tooth pulp cells, was employed for this research. The α-、β-、γ- and δ-cellswere obtained after pancreatic differentiation. After hepatic differentiation all hepatic markers were positive in the most of cells, moreover glycogen accumulation and urea production, which are specific function in hepatic cells, were found.
We developed a novel serum-free media for hepatic differentiation. After implantation of the cells differentiated in the serum-free into immunosuppressive rats, we found successful implantation, proliferation and the formation of blood vessels. Moreover we produced biliary liver cirrhosis in nude rats and transplanted the cells, and then the condition was dramatically improved. No cirrhosis was found.
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