Contribution of post-translational modifications of tubulins in neuronal morphogenesis

• Project/Area Number: 23500443
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: University of Fukui
• Principal Investigator: KONISHI Yoshiyuki 福井大学, 工学(系)研究科(研究院), 准教授 (00382838)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 微小管 / 翻訳後修飾 / 軸索 / 極性 / 樹状突起 / チュブリン / シグナル伝達 / 神経極性 / 細胞移動 / 神経細胞 / 神経 / ニューロフィラメント

Outline of Final Research Achievements

Microtubules play central roles in the regulation of cellular morphology. It could receive region dependent regulation within the cell, which is especially important in the regulation of complicated neuronal cell shape. In this project, we investigated the role of two types of post-translational modifications of tubulins, tyrosination and polyglutamylation in the regulation of neuronal structure. Especially, we found that polyglutamylation regulate the dendritic distribution of neurofilaments. In addition to these tubulin modifications, we investigated the neuronal subtype dependent regulation of morphogenesis.

Research Products

• [Journal Article] A low-density culture method of cerebellar granule neurons with paracrine support applicable for the study of neuronal morphogenesis (2013)
  • Author(s): Kubota K, Seno T, Konishi Y
  • Journal Title: Brain Res. Volume: 1539 Pages: 15-23
  • DOI: 10.1016/j.brainres.2013.09.046
  • Peer Reviewed
• [Journal Article] Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics (2012)
  • Author(s): Yang H, Sugiura Y, Ikegami K, Konishi Y, Setou M
  • Journal Title: J Biol Chem Volume: 287巻 Issue: 8 Pages: 5290-5300
  • DOI: 10.1074/jbc.m111.316877
  • Peer Reviewed
• [Presentation] 神経細胞の形態形成における細胞種依存的なGSK-3の機能 (2014)
  • Author(s): 小西慶幸, 久保田健太, 衞藤拓也
  • Organizer: 第36回日本生物学的精神医学会、第57回日本神経化学会大会
  • Place of Presentation: 奈良市
  • Year and Date: 2014-09-29
• [Presentation] Local regulation of molecular reactions in maintaining axonal morphology (2013)
  • Author(s): Konishi Y, Seno T, Kubota K, Sakae N, Takada H
  • Organizer: 7th International Symposium on Nanomedicine
  • Place of Presentation: Kitakyushu, Japan
  • Invited
• [Presentation] Molecular network analysis of neuronal polarity in cerebellar granule neurons (2013)
  • Author(s): Seno T, Kubota K, Sakae N, Konishi Y
  • Organizer: Neuro2013
  • Place of Presentation: Kyoto, Japan
• [Presentation] Functional analysis of GSK3beta in cerebellar granule neurons by using new method of low-density culture (2013)
  • Author(s): Kubota K, Seno T, Konishi Y
  • Organizer: Neuro2013
  • Place of Presentation: Kyoto, Japan
• [Presentation] Spatial cell signaling mediated by microtubule regulations contributes to the maintenance of neuronal morphology (2012)
  • Author(s): Yoshiyuki Konishi
  • Organizer: The 11th biennial meeting of APSN and the 55th annual meeting of JSN
  • Place of Presentation: Kobe, Japan
• [Presentation] 神経細胞の形づくりの機構－分子細胞生物学的視点から (2012)
  • Author(s): 小西慶幸
  • Organizer: 形の科学会
  • Place of Presentation: 福井
• [Presentation] .軸索形態制御に関わる翻訳後修飾を介した細胞内分子機構 (2011)
  • Author(s): 小西慶幸
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 石川
• [Remarks] 福井大学 知能システム工学科 ホームページ
  • URL: http://kou25hp.eng.u-fukui.ac.jp/his/
• [Remarks] 福井大学 教育研究者情報
  • URL: http://t-profile.u-fukui.ac.jp/ufids/servlet/RefOutController?exeBO=WR0000SBO&monitorID=WR4100S
• [Remarks] 福井大学知能システム工学科ホームページ
  • URL: http://kou25hp.eng.u-fukui.ac.jp/his/