| Project/Area Number |
23500967
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | University of Hyogo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUKUI Toru 兵庫県立大学, 環境人間学部, 研究員 (50425443)
EBARA Shuhei 兵庫県立大学, 環境人間学部, 助教 (10372856)
SAWAMURA Hiromi 兵庫県立大学, 環境人間学部, 助手 (30555371)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ビオチン / 異化代謝物 / 高速液体クロマトグラフィ / ビオチンスルホキシド / ビオチンスルホン / ビスノルビオチン / ヒドロキシイソ吉草酸 / HPLC / ３－ヒドロキシイソ吉草酸 / アビジン / 尿 / 血清 / 栄養状態 |
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| Outline of Final Research Achievements |
As for biotin which is one of water-soluble vitamins, the standard method for determination has not been established as yet. Biotin sulfoxide, biotin sulfone and bisnorbiotin are excreted in the urine as catabolisms of biotin, and hydroxyisovaleric acid is known as an indicator of biotin deficiency. In this study the analytical method to determine simultaneously these biotin-related substances using a high performance liquid chromatography (HPLC) was studied. The methods labeling the carboxylic acid of these substances and the separation conditions were determined. As a result, the determined method labeled with a fluorescent dye was the most available. However, in the analysis of biological samples, because the contaminants such as proteins are contained much, it was difficult to detect even sample was concentrated. This HPLC method as the standard method for determining biotin and its related substances can not be proposed as yet.
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