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Analyses of ER-resident molecular chaperone-dependent nuclear membrane fusion during fertilization of Arabidopsis thaliana

Research Project

Project/Area Number 23570051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNiigata University (2012-2013)
Nagoya University (2011)

Principal Investigator

NISHIKAWA Shuh-ichi  新潟大学, 自然科学系, 教授 (10252222)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords小胞体 / 分子シャペロン / 核膜融合 / 有性生殖 / シロイヌナズナ
Research Abstract

We showed that BiP, an Hsp70 molecular chaperone in the endoplasmic reticulum, function in the fusions of the outer and inner nuclear membranes of the polar nuclei with different sets of J-domain containing partner proteins during the female gametogenesis of A. thaliana. Live imaging analyses showed that the polar nuclei fusion defect can be recovered during the first endosperm nuclear division after fertilization and that the fusion of sperm nuclei at fertilization is required for proper endosperm proliferation. We also identified candidates of nuclear membrane proteins required for the polar nuclei fusion.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (33 results)

All 2014 2013 2012 2011

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (14 results) (of which Invited: 2 results) Book (4 results)

  • [Journal Article] Multiple BiP genes of Arabidopsis thaliana are required for male gametogenesis and pollen competitiveness2014

    • Author(s)
      Maruyama, D., Sugiyama, T., Endo, T., and Nishikawa, S.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Pages: 801-810

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Plant Organelles Database 3 (PODB3) update 2014: integrating electron micrographs and new options for plant organelle research2014

    • Author(s)
      Mano, S., Nakamura, T., Kondo, M., Miwa, T., Nishikawa, S., Mimura, T., Nagatani, A., and Nishimura, M.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Plant Organelles Database 3 (PODB3) update 2014: integrating electron micrographs and new options for plant organelle research.2014

    • Author(s)
      Mano S, Nakamura T, Kondo M, Miwa T, Nishikawa S, Mimura T, Nagatani A, Nishimura M.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 1 Pages: e1-e1

    • DOI

      10.1093/pcp/pct140

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiple BiP genes of Arabidopsis thaliana are required fbr male gametogenesis and pollen competitiveness.2014

    • Author(s)
      Daisuke Maruyama, Tomoyuki Sugiyama, Toshiya Endo, Shuh-ichi Nishikawa
    • Journal Title

      Plant & Cell Physiology

      Volume: 55 Issue: 4 Pages: 801-810

    • DOI

      10.1093/pcp/pcu018

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analyses of protein-protein interactions by in vivo photocrosslinking in budding yeast2013

    • Author(s)
      Shiota,T., Nishikawa, S., and Endo, T.
    • Journal Title

      Meth, Mol. Biol.

      Volume: 1033 Pages: 207-217

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria2013

    • Author(s)
      Tamura, Y., Harada, Y., Nishikawa, S., Yamano, K., Kamiya, M., Shiota, T., Kuroda, T., Kuge, O., Sesaki, H., Imai, K., Tomii, K., and Endo, T.
    • Journal Title

      Cell Metab.

      Volume: 17 Pages: 709-718

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria2013

    • Author(s)
      Y. Tamura, Y. Harada, S. Nishikawa, K. Yamano, M. Kamiya, T. Shiota, T. Kuroda, O. Kuge, H. Sesaki, K. Imai, K. Tomii, and T. Endo
    • Journal Title

      Cell Metab.

      Volume: 17 Issue: 5 Pages: 709-718

    • DOI

      10.1016/j.cmet.2013.03.018

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analyses of protein-protein interactions by in vivo photocrosslinking in budding yeast.2013

    • Author(s)
      塩田拓也、西川周一
    • Journal Title

      Meth. Mol. Biol.

      Volume: 1033 Pages: 207-217

    • DOI

      10.1007/978-1-62703-487-6_14

    • ISBN
      9781627034869, 9781627034876
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analyses of protein-protein interactions by in vivo photocrosslinking in budding yeast.2013

    • Author(s)
      塩田拓也
    • Journal Title

      Methods Mol. Biol.

      Volume: -

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria2013

    • Author(s)
      田村康
    • Journal Title

      Cell Metabol.

      Volume: -

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of Dom34:Hbs1 in non-stop protein clearance from translocators for normal organelle protein influx2012

    • Author(s)
      Izawa, T., Tsuboi, T., Kuroha, K., Inada, T., Nishikawa, S., and Endo, T.
    • Journal Title

      Cell Rep.

      Volume: 2 Pages: 447-453

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Yos9p and Hrd1p mediate ER retention of misfolded proteins for ER- associated degradation2012

    • Author(s)
      Izawa, T., Nagai, H., Endo, T. and Nishikawa, S.
    • Journal Title

      Mol. Biol. Cell

      Volume: 23 Pages: 1283-1293

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of dom34 : hbs1 in nonstop protein clearance from translocators for normal organelle protein influx.2012

    • Author(s)
      Toshiaki Izawa, Tatsuhisa Tsuboi, Kazushige Kuroha, et al.
    • Journal Title

      Cell Reports

      Volume: 2 Issue: 3 Pages: 447-453

    • DOI

      10.1016/j.celrep.2012.08.010

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Yos9p and Hrd1p mediate ER retention of misfolded proteins for ER-associated degradation2012

    • Author(s)
      T. Izawa, H. Nagai, T. Endo, and S. Nishikawa
    • Journal Title

      Mol. Biol. Cell

      Volume: 23 Issue: 7 Pages: 1283-1293

    • DOI

      10.1091/mbc.e11-08-0722

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Plant Organelles Database 2 (PODB2) : An update containing movie data of plant organelle dynamics2011

    • Author(s)
      Mano Shoji
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Issue: 2 Pages: 244-253

    • DOI

      10.1093/pcp/pcq184

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 高温ストレス下での花粉成熟過程における小胞体品質管理の役割2014

    • Author(s)
      杉山智之, 山本雅也, 遠藤斗志也, 西川周一
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学,富山県
    • Year and Date
      2014-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 高温ストレス下での花粉成熟過程における小胞体品質管理の役割2014

    • Author(s)
      杉山智之、西川周一
    • Organizer
      第55回 日本植物生理学会大会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] ER quality control plays key roles in sexual reproduction at an elevated temperature in Arabidopsis thaliana2013

    • Author(s)
      Tomoyuki Sugiyamaa, Masaya Yamamotob, Toshiya Endo and Shuh-ichi Nishikawa
    • Organizer
      The 3rd International Congress on Natural Sciences
    • Place of Presentation
      新潟大学五十嵐キャンパス,新潟県
    • Year and Date
      2013-10-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 小胞体における異常タンパク質の処理機構:分子機構と高次機能2013

    • Author(s)
      西川周一
    • Organizer
      新潟生化学懇話会
    • Place of Presentation
      新潟大学脳研究所,新潟県
    • Year and Date
      2013-07-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 雌性配偶体形成時の極核の核外膜融合は中央細胞における精核の融合と胚乳形成に必要である2013

    • Author(s)
      丸山大輔, 山本雅也, 東山哲也, 遠藤斗志也, 西川周一
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学,岡山県
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 小胞体における異常タンパク質の処理機構:分子機構と高次機能2013

    • Author(s)
      西川周一
    • Organizer
      新潟生化学懇話会
    • Place of Presentation
      新潟大学脳研究所
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ER quality control plays key roles in sexual reproduction at an elevated temperature in Arabidopsis thaliana.2013

    • Author(s)
      杉山智之、西川周一
    • Organizer
      The 3rd International Congress on Natural Sciences
    • Place of Presentation
      新潟大学五十嵐キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 雌性配偶体形成時の極核の核外膜融合は中央細胞における精核の融合と胚乳形成に必要である2013

    • Author(s)
      西川周一
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 植物の環境ストレス応答における小胞体品質管理の役割2012

    • Author(s)
      西川周一
    • Organizer
      第12回日本蛋白質科学会年会ワークショップ「植物の環境感覚をささえる多様な蛋白質機能」
    • Place of Presentation
      名古屋国際会議場,愛知県
    • Year and Date
      2012-06-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物の環境ストレス応答における小胞体品質管理の役割2012

    • Author(s)
      西川周一
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Regulation of plant growth and development by the Hsp70 system in the ER.2011

    • Author(s)
      Shuh-ichi Nishikawa
    • Organizer
      「奈良先端未来開拓コロキウム」シンポジウムProtein and Organelle Dynamics under Cellular Stress Conditions
    • Place of Presentation
      奈良先端科学技術大学院大学バイオサイエンス研究科,奈良県
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of plant growth and development by the Hsp70 system in the ER.2011

    • Author(s)
      西川周一
    • Organizer
      「奈良先端未来開拓コロキウム」シンポジウムProtein and Organelle Dynamics under Cellular Stress Conditions(招待講演)
    • Place of Presentation
      奈良先端科学技術大学院大学バイオサイエンス研究科
    • Related Report
      2011 Research-status Report
  • [Presentation] 植物の発生・生育において小胞体品質管理機構がはたす役割は何か2011

    • Author(s)
      西川周一
    • Organizer
      第29回日本植物細胞分子生物学会大会 シンポジウム(招待講演)
    • Place of Presentation
      九州大学箱崎キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] Substrate sorting and selection in ER-associated degradation2011

    • Author(s)
      西川周一
    • Organizer
      Global-COE Mini-Symposium -Toward System Glycobiology- Biosynthesis and Catabolism of Glycoproteins(招待講演)
    • Place of Presentation
      名古屋大学
    • Related Report
      2011 Research-status Report
  • [Book] Using image-based resources : Databases for plant organelle dynamics and applications based on image information. Image and Video Processing2013

    • Author(s)
      Mano, S., Kimori, S., Takeda, T., Miwa, T., Nishikawa, S., Mimura, T., Nagatani, A., and Nishimura, M.
    • Publisher
      iConcept Press(ISBN : 978-1-477554-80-7)
    • Related Report
      2013 Final Research Report
  • [Book] Image and Video Processing - An Introductory Guide (Chapter 5)2013

    • Author(s)
      真野昌二、西川周一
    • Total Pages
      326
    • Publisher
      iConcept press
    • Related Report
      2013 Annual Research Report
  • [Book] Image and Video Processing2013

    • Author(s)
      真野昌二
    • Publisher
      iConcept Press.
    • Related Report
      2012 Research-status Report
  • [Book] 動的なタンパク質相互作用の「スナップショット」を撮る〜in vivoにおける部位特異的光架橋法2012

    • Author(s)
      塩田拓也, 西川周一, 遠藤斗志也
    • Publisher
      実験医学
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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