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Elucidation of reproductive endocrine system in hagfish

Research Project

Project/Area Number 23570072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionNiigata University

Principal Investigator

NOZAKI Masumi  新潟大学, 自然科学系, 教授 (70136232)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsヌタウナギ / 無顎類 / 下垂体 / 生殖腺刺激ホルモン / 性ステロイドホルモン / コレステロール側鎖切断酵素 / ステロイド産生細胞 / 退化卵胞
Research Abstract

Since hagfishes are considered the most primitive vertebrate known, living or extinct, studies on their reproduction are important for understanding the evolutionary aspects of the vertebrate reproductive endocrine system. Recently, single functional GTH was identified in the hagfish pituitary gland. In the present study, cytochrome P450 side-chain cleavage enzyme (CYP11A) was cloned from the hagfish testis. A clear positive correlation was noted between CYP11A mRNA levels and gonadal development in both sexes. Moreover, hagfish GTH stimulated CYP11A mRNA levels in the cultured testis. A PQRFamide peptide identified from the hagfish hypothalamus was shown to stimulate GTH mRNA levels in the hagfish pituitary. The present study further showed the presence of a steroid (estradiol) feedback system at the hypothalamic-pituitary levels. It is suggested that vertebrates, during their early evolution, have established the hypothalamic-pituitary-gonadal axis.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (30 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (21 results) (of which Invited: 3 results) Remarks (2 results)

  • [Journal Article] Hypothalamic-pituitary-gonadal endocrine system in the hagfish.2013

    • Author(s)
      Nozaki, M.
    • Journal Title

      Front. Endocrinol.

      Volume: 4 Pages: 200-200

    • DOI

      10.3389/fendo.2013.00200

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Relationships between plasma concentrations of sex steroid hormones and gonadal development in the brown hagfish2013

    • Author(s)
      Nishiyama M, Chiba H, Uchida K, Shimotani T, Nozaki M
    • Journal Title

      Paramyxine atami, Zool Sci

      Volume: 30 Issue: 11 Pages: 967-974

    • DOI

      10.2108/zsj.30.967

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of estradiol or testosterone treatment on expression of gonadotropin subunit mRNAs and proteins in the pituitary of juvenile brown hagfish, Paramyxine atami2013

    • Author(s)
      Nozaki, M., Uchida, K., Honda, K., Shimotani, T., Nishiyama, M.
    • Journal Title

      Gen. Comp. Endocrinol.

      Volume: 189 Pages: 111-118

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glycoprotein hormone in the pituitary of hagfish and its evolutionary implications2013

    • Author(s)
      K. Uchida, S. Moriyama, S. SowerA., M. Nozaki
    • Journal Title

      Fish Physiol. Biochem.

      Volume: 39 Issue: 1 Pages: 75-83

    • DOI

      10.1007/s10695-012-9657-6

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evolutionary origin of the structure and function of gonadotropin-inhibitory hormone : Insights from lampreys2012

    • Author(s)
      T.Osugi, T.Ubuka, M.Nozaki, S.A.Sower, K.Tsutsui, et al
    • Journal Title

      Endocrinology

      Volume: (印刷中)(in~press) Issue: 5 Pages: 2362-2374

    • DOI

      10.1210/en.2011-2046

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hagfish, genome duplications, and RFamide neuropeptide evolution2011

    • Author(s)
      T.Osugi, K.Tsutsui, et al
    • Journal Title

      Endocrinology

      Volume: 152 Issue: 11 Pages: 4010-4013

    • DOI

      10.1210/en.2011-1375

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of novel RFamide peptides in the central nervous system of the brown hagfish: Isolation, localization and functional analysis.2011

    • Author(s)
      Osugi, T., Uchida, K., Nozaki, M., Tsutsui, K.
    • Journal Title

      Endocrinology

      Volume: 152 Pages: 4252-4264

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ヌタウナギからみた下垂体の進化。小林英司先生記念シンポジウム「比較内分泌学の創成と発展」2013

    • Author(s)
      野崎眞澄
    • Organizer
      第38回日本比較内分泌学会大会及びシンポジウム
    • Place of Presentation
      宮崎
    • Related Report
      2013 Final Research Report
  • [Presentation] クロヌタウナギのおける生殖腺発達に応じた血中性ステロイドホルモン動態と合成酵素の探索2013

    • Author(s)
      西山真樹, 内田勝久, 森山俊介, 千葉洋明, 阿部希美, 下谷豊和, 野崎眞澄
    • Organizer
      第38回日本比較内分泌学会大会及びシンポジウム
    • Place of Presentation
      宮崎
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ヌタウナギからみた腺下垂体ホルモンの進化。シンポジウム「下垂体の起源と進化」2013

    • Author(s)
      野崎眞澄
    • Organizer
      日本下垂体研究会第28回学術集会
    • Place of Presentation
      花巻
    • Related Report
      2013 Final Research Report
  • [Presentation] クロヌタウナギにおける生殖腺発達に応じた血中性ステロイドホルモン動態と合成酵素の探索2013

    • Author(s)
      西山真樹, 阿部希美, 内田勝久, 下谷豊和, 野崎眞澄
    • Organizer
      日本下垂体研究会第28回学術集会
    • Place of Presentation
      花巻
    • Related Report
      2013 Final Research Report
  • [Presentation] ヌタウナギからみた視床下部ー下垂体ー生殖腺軸の進化。セッション「境界動物の内分泌現象」2013

    • Author(s)
      野崎眞澄
    • Organizer
      東京大学大気海洋研究所共同利用研究集会「海洋生物の様々な適応戦略」
    • Place of Presentation
      柏
    • Related Report
      2013 Final Research Report
  • [Presentation] Hypothalamic-pituitary- gonadal endocrine system in the hagfish2013

    • Author(s)
      Nozaki, M.
    • Organizer
      Deep Sequencing at CDB
    • Place of Presentation
      Kobe
    • Related Report
      2013 Final Research Report
  • [Presentation] ヌタウナギからみた腺下垂体ホルモンの進化2013

    • Author(s)
      野崎眞澄
    • Organizer
      日本下垂体研究会 第28回学術集会
    • Place of Presentation
      花巻
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] クロヌタウナギにおける生殖腺発達に応じた血中性ステロイドホルモン動態と合成酵素の探索2013

    • Author(s)
      西山真樹・阿部希美・内田勝久・下谷豊和・野崎眞澄
    • Organizer
      日本下垂体研究会 第28回学術集会
    • Place of Presentation
      花巻
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヌタウナギからみた下垂体の進化2013

    • Author(s)
      野崎眞澄
    • Organizer
      第38回日本比較内分泌学会大会及びシンポジウム
    • Place of Presentation
      宮崎
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] クロヌタウナギ下垂体のGTHmRNAレベルならびにGTHタンパク質レベルに対するエストロジェン投与の効果2012

    • Author(s)
      野崎眞澄, 本田香織, 内田勝久, 下谷豊和, 西山真樹
    • Organizer
      日本下垂体研究会第27回学術集会
    • Place of Presentation
      花巻
    • Related Report
      2013 Final Research Report
  • [Presentation] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish2012

    • Author(s)
      Nozaki, M., Shimotani, T., Nishiyama, M., Uchida, K.
    • Organizer
      The 7th Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Related Report
      2013 Final Research Report
  • [Presentation] Study on sex steroid hormones in the hagfish - Their serum concentrations and sex steroid biosynthetic enzymes2012

    • Author(s)
      Nishiyama, M., Uchida, K., Moriyama, S., Chiba, H., Awata, S., Nozaki, M.
    • Organizer
      The 7th Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Related Report
      2013 Final Research Report
  • [Presentation] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish.2012

    • Author(s)
      Nozaki, M., Shimotani, T., Nishiyama, M., Uchida, K.
    • Organizer
      7th Congress of the Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      Kuala Lumpur
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Study on sex steroid hromones in the hagfish: Their serum concentrations and sex steroid biosynthetic enzymes.2012

    • Author(s)
      Nishiyama, M., Uchida, K., Moriyama, S., Chiba, H., Awata, S., Nozaki, M.
    • Organizer
      7th Congress of the Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      Kuala Lumpur
    • Related Report
      2012 Research-status Report
  • [Presentation] クロヌタウナギ下垂体のGTH mRNAレベルならびにGTHタンパク質レベルに対するエストロジェン投与の効果。2012

    • Author(s)
      野崎眞澄・本田香織・内田勝久・下谷豊和・西山真樹
    • Organizer
      日本下垂体研究会第27回学術集会
    • Place of Presentation
      天童
    • Related Report
      2012 Research-status Report
  • [Presentation] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish.2012

    • Author(s)
      Nozaki, M., Shimotani, T., Nishiyama, M., Uchida, K.
    • Organizer
      7th Congress of the Asia and Oceania Society for Comparative Endocrinology(招待講演)
    • Place of Presentation
      クアラルンプール(マレーシア)
    • Related Report
      2011 Research-status Report
  • [Presentation] Study on sex steroid hormones in the hagfish - Their serum concentrations and sex steroid biosynthetic enzymes.2012

    • Author(s)
      Nishiyama, M., Uchida, K., Moriyama, S., Chiba, H., Awata, S., Nozaki, M.
    • Organizer
      7th Congress of the Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      クアラルンプール(マレーシア)
    • Related Report
      2011 Research-status Report
  • [Presentation] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish2011

    • Author(s)
      Nozaki, M.
    • Organizer
      2nd JAMBIO Forum
    • Place of Presentation
      Shimoda Marine Res. Center, Univ. of Tsukuba
    • Year and Date
      2011-01-21
    • Related Report
      2013 Final Research Report
  • [Presentation] クロヌタウナギにおける性ステロイド合成酵素の探索2011

    • Author(s)
      西山真樹, 内田勝久, 森山俊介, 千葉洋明, 下谷豊和, 安房田智司, 野崎眞澄
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 吉村賞受賞者講演「無顎類からみた下垂体の進化に関する研究」2011

    • Author(s)
      野崎眞澄
    • Organizer
      日本下垂体研究会第26回学術集会
    • Place of Presentation
      せとうち児島ホテル、岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] 無顎類からみた下垂体の進化に関する研究2011

    • Author(s)
      野崎眞澄
    • Organizer
      日本下垂体研究会第26回学術集会(招待講演)
    • Place of Presentation
      せとうち児島ホテル(岡山)
    • Related Report
      2011 Research-status Report
  • [Remarks] 1)新潟大学ホームページ

    • URL

      http://www.niigata-u.ac.jp/top/pickup/230926_1.html

    • Related Report
      2011 Research-status Report
  • [Remarks] 2)新潟大学理学部ホームページ

    • URL

      http://www.sc.niigata-u.ac.jp/sc/pub/maga/No29.pdf

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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