| Project/Area Number |
23570081
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphology/Structure
|
|---|
| Research Institution | Rakuno Gakuen University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAIYA Hiroyuki 独立法人国立循環器病研究センター, 研究所, 室長 (40300975)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SAKAI Takafumi 埼玉大学, 理工学研究科, 教授 (40235114)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
|---|
| Keywords | グレリン / モチリン / 受容体 / グレリン合成酵素 / ニワトリ消化管 / 遺伝子発現調整機構 / 収縮機能 / ニワトリ / 腺胃 / グレリンmRNA / グレリン合成酵素mRNA / グレリン受容体(GHS-R) mRNA / 成長 / 中枢神経 / 消化管収縮性 / 受容体発現 / 内因性グレリン / 消化管 / 成長過程 / グレリン受容体(GHS-R) / モチリン受容体 |
|---|
| Research Abstract |
Ghrelin contracted a chicken gastrointestinal tract. The ghrelin-induced contraction and expression of growth hormone secretagogue receptor (GHS-R1a) mRNA in the proventriculus decreased depending on age. Ghrelin concentrations in the proventriculus and plasma increased after hatching and reached plateau at 30 days. Expression of ghrelin and ghrelin-O-acetyltransferase (GOAT) mRNAs also tended to decrease depending on age. Expression of ghrelin mRNA did not show any correlation with plasma ghrelin. Inverse relationships between ghrelin concentration and GHS-R1a and GOAT mRNAs expression are indicative for a ghrelin-induced downregulation of the ghrelin receptor and synthetic enzyme. The expression pattern of ghrelin, GOAT and GHS-R1a mRNAs in the brain distinctly change in region-dependent manner on growing stage. The present results indicated that physiological role of ghrelin system changes in the brain and gastrointestinal tract of growing chickens.
|
