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Lipids are critical for formation of pollen coat.

Research Project

Project/Area Number 23570082
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionJapan Women's University

Principal Investigator

NAGATA Noriko  日本女子大学, 理学部, 准教授 (40311352)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords植物 / シロイヌナズナ / 花粉 / 葯 / 電子顕微鏡 / 脂質 / 資質 / ポーレンコート / タペータム
Research Abstract

Male gametophyte in higher plants contains characteristic lipid-rich organelles. Much of the pollen coat material derives from two tapetal lipid-rich organelles: tapetosome and elaioplast. The analysis of lipid components of tapetosome, elaioplast, male gametophyte and pollen coat revealed that they package a defined series of lipid components.
To understand the mechanism for formation of these lipid-rich organelles, I made an organelle-map using transmission electron microscopy during wild-type Arabidopsis pollen development. Furthermore, I analyzed the Arabidopsis mutants of lipid metabolism and described the role of lipids on the male gametophyte and tapetum development. It is becoming clear that the lipids are critical on the male gametophyte development by reverse-genetic strategy using Arabidopsis.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (33 results)

All 2014 2013 2012 2011 Other

All Journal Article (16 results) (of which Peer Reviewed: 12 results,  Open Access: 2 results) Presentation (15 results) Book (1 results) Remarks (1 results)

  • [Journal Article] シロイヌナズナでのクロロフィル形成と環境条件との関係2014

    • Author(s)
      渡邊絵梨, 清水麻里, 小澤あつみ, 永田典子, 今井元
    • Journal Title

      日本女子大学紀要理学部

      Volume: 第22号 Pages: 13-17

    • NAID

      120005572316

    • Related Report
      2013 Final Research Report
  • [Journal Article] Photoassimilation, assimilate translocation and plasmodesmal biogenesis in the source leaves of Arabidopsis thaliana grown under an increased atmospheric CO2 concentration.2014

    • Author(s)
      Z. Duan, A. Homma, M. Kobayashi, N. Nagata, Y. Kaneko, Y. Fujiki, I. Nishida
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 2 Pages: 358-369

    • DOI

      10.1093/pcp/pcu004

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development2014

    • Author(s)
      Takamitsu Kurusu, Tomoko Koyano, Shigeru Hanamata, Takahiko Kubo, Yuhei Noguchi, Chikako Yagi, Noriko Nagata, Takashi Yamamoto, Takayuki Ohnishi, Yozo Okazaki, Nobutaka Kitahata, Daichi Ando, Masaya Ishikawa, Shinya Wada, Akio Miyao, Hirohiko Hirochika, Hiroaki Shimada, Amane Makino, Kazuki Saito, Hiroyuki Ishida, Tetsu Kjnoshita, Nori Kurata and Kazuyuki Kuchitsu^*
    • Journal Title

      Autophagy

      Volume: 10(5) Issue: 5 Pages: 878-888

    • DOI

      10.4161/auto.28279

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phosphatidylglycerol biosynthesis is required for the development of embryos and normal membrane structures of chloroplasts and mitochondria in Arabidopsis2014

    • Author(s)
      R. Tanoue, M. Kobayashi, K. Katayama, N. Nagata, H. Wada
    • Journal Title

      FEBS letter

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylglycerol biosynthesis is required for the development of embryos and normal membrane structures of chloroplasts and mitochondria in Arabidopsis.2014

    • Author(s)
      Ryo Tanoue, Megumi Kobayashi, Kenta Katayama, Noriko Nagata, Hajime Wada
    • Journal Title

      FEBS Letters

      Volume: (掲載確定) Issue: 9 Pages: 1680-1685

    • DOI

      10.1016/j.febslet.2014.03.010

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 葉緑体タンパク質をコードする遺伝子変異体の表現型データベースの紹介2013

    • Author(s)
      明賀史純, 永田典子
    • Journal Title

      Perceptive Plants

      Volume: Vol.5 Pages: 19-19

    • Related Report
      2013 Final Research Report
  • [Journal Article] Development of Capsicum EST-SSR markers for species identification and in silico mapping onto the tomato genome sequence2013

    • Author(s)
      K. Shirasawa, K. Ishii, C. Kim, T. Ban, M. Suzuki, T. Ito, T. Muranaka, M. Kobayashi, N. Nagata, S. Isobe, S. Tabata
    • Journal Title

      Molecular Breeding

      Volume: 31 Pages: 101-110

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Chloroplast Function Database II : A comprehensive collection of homozygous mutants and their phenotypic/genotypic traits for nuclear-encoded chloroplast proteins2013

    • Author(s)
      F. Myouga, K. Akiyama, Y. Tomonaga, A. Kato, Y. Sato, M. Kobayashi, N. Nagata, T. Sakurai, K. Shinozaki
    • Journal Title

      Plant Cell Physiology

      Volume: 54(2), e2 Pages: 1-10

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Very-Long-Chain Fatty Acids in the Epidermis Controls Plant Organ Growth by Restricting Cell Proliferation2013

    • Author(s)
      T. Nobusawa, Y. Okushima, N. Nagata, M. Kojima, H. Sakakibara, M. Umeda
    • Journal Title

      PLOS Biology

      Volume: 11, 4

    • NAID

      120005716835

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Restriction of cell proliferation in internal tissues via the synthesis of very-long-chain fatty acids in the epidermis2013

    • Author(s)
      Nobusawa, T., Okushima, Y., Nagata, N., Kojima, M., Sakakibara, H. and Umeda, M.
    • Journal Title

      Plant Signal. Behav.

      Volume: 8 Issue: 8 Pages: 8-8

    • DOI

      10.4161/psb.25232

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of very-long-chain fatty acids in the epidermis controls plant organ growth by restricting cell proliferation2013

    • Author(s)
      Nobusawa, T., Okushima, Y., Nagata, N., Kojima, M., Sakakibara, H. and Umeda, M.
    • Journal Title

      PLoS Biol.

      Volume: 11 Issue: 4 Pages: 1-14

    • DOI

      10.1371/journal.pbio.1001531

    • NAID

      120005716835

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis mitochondrial protein TIM50 affects hypocotyl cell elongation through intracellular ATP level2012

    • Author(s)
      S. Kumar, T. Yoshizumi, H. Hongo, A. Yoneda, H. Hara, H. Hamasaki, N. Takahashi, N. Nagata, H. Shimada, M. Matsui
    • Journal Title

      Plant Science

      Volume: 183 Pages: 212-217

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] スペクトル観測によるクロロフィル成長過程の検討2012

    • Author(s)
      小澤あつみ, 渡邊絵梨, 永田典子, 今井元
    • Journal Title

      電子情報通信学会誌

      Volume: vol.J95-C, no.10 Pages: 228-230

    • NAID

      110009517414

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis mitochondrial protein TIM50 affects hypocotyl cell elongation through intracellular ATP level.2012

    • Author(s)
      Shailesh Kumar, Takeshi Yoshizumi, Hiroaki Hongo, Arata Yoneda, Hiroko Hara, Hidefumi Hamasaki, Naoki Takahashi, Noriko Nagata, Hiroaki Shimada, Minami Matsui
    • Journal Title

      Plant Science

      Volume: 183 Pages: 212-217

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 被子植物の細胞質遺伝2011

    • Author(s)
      永田典子
    • Journal Title

      Plant Morphology

      Volume: 23 Pages: 25-33

    • NAID

      130002152895

    • Related Report
      2013 Final Research Report
  • [Journal Article] 高圧凍結技法を取り入れた広域透過電顕像自動取得システムの開発とその応用

    • Author(s)
      豊岡公徳, 佐藤繭子, 朽名夏麿, 永田典子
    • Journal Title

      Plant Morphology

      Volume: (in press)

    • NAID

      130005066550

    • Related Report
      2013 Final Research Report
  • [Presentation] 透過型電子顕微鏡画像に基づく葉緑体微細構造異常の自動認識2014

    • Author(s)
      桧垣匠, 加藤綾, 明賀史純, 朽名夏麿, 馳澤盛一郎, 永田典子
    • Organizer
      日本顕微鏡学会関東支部第38回講演会
    • Place of Presentation
      日本女子大,東京
    • Year and Date
      2014-03-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 透過電顕像広域自動撮影システムの開発と植物組織への応用2014

    • Author(s)
      豊岡公徳, 佐藤繭子, 朽名夏麿, 若崎眞由美, 澤木史江, 桧垣匠, 馳澤盛一郎, 永田典子, 松岡健
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大,富山
    • Related Report
      2013 Final Research Report
  • [Presentation] 広域透過電子顕微鏡画像取得法の開発とその応用2013

    • Author(s)
      澤木史江, 小林恵, 佐藤繭子, 朽名夏麿, 桧垣匠, 馳澤盛一郎, 豊岡公徳, 永田典子
    • Organizer
      第37回日本顕微鏡学会関東支部講演会
    • Place of Presentation
      東京,東大
    • Year and Date
      2013-03-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Chloroplast Function Database IIは核コード葉緑体蛋白質変異体の網羅的収集データと変異体の表現型データとを提供する2013

    • Author(s)
      明賀史純, 秋山顕治, 朝長優美, 加藤綾, 佐藤由佳, 小林恵, 永田典子, 櫻井哲也, 篠崎一雄
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大,岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] 電顕アトラス:高圧凍結技法を取り入れた広域電顕像撮影システムの開発2013

    • Author(s)
      佐藤繭子, 朽名夏麿, 澤木史江, 若崎眞由美, 桧垣匠, 吉田拓弘, 櫻井哲也, 馳澤盛一郎, 持田恵一, 永田典子, 豊岡公徳
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大,岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナ葉緑体突然変異体のデータベース構築に向けた網羅的な透過電子顕微鏡解析2013

    • Author(s)
      加藤綾, 明賀史純, 秋山顕治, 佐藤由佳, 小林恵, 櫻井哲也, 篠崎一雄, 永田典子
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      大阪,ホテル阪急エキスポパーク
    • Related Report
      2013 Final Research Report
  • [Presentation] タバコ根端分裂組織における液胞膜タンパク質V-PPaseの分布と液胞形成2013

    • Author(s)
      佐藤繭子, 後藤友美, 若崎眞由美, 朽名夏麿, 澤木史江, 永田典子, 松岡健, 豊岡公徳
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      大阪,ホテル阪急エキスポパーク
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉緑体突然変異体の透過電子顕微鏡画像のデータベース構築2013

    • Author(s)
      加藤綾, 明賀史純, 秋山顕治, 小林恵, 櫻井哲也, 篠崎一雄, 永田典子
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道,北大
    • Related Report
      2013 Final Research Report
  • [Presentation] 広域TEM画像取得法の開発と茎頂オルガネラ地図作成への応用2013

    • Author(s)
      澤木史江, 小林恵, 佐藤繭子, 朽名夏麿, 桧垣匠, 馳澤盛一郎, 豊岡公徳, 永田典子
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道,北大
    • Related Report
      2013 Final Research Report
  • [Presentation] 生体顕微マルチスケーリングマッピングシステム:小胞クラスターの分布と構造2012

    • Author(s)
      豊岡公徳, 若崎眞由美, 朽名夏麿, 桧垣匠, 永田典子, 松岡健, 持田恵一, 佐藤繭子
    • Organizer
      日本顕微鏡学会第67回学術講演会
    • Place of Presentation
      つくば,つくば国際会議場
    • Year and Date
      2012-05-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 生体顕微マルチスケーリングマッピング:小胞クラスターの分布と超微形態2012

    • Author(s)
      豊岡公徳, 若崎眞由美, 吉田拓広, 朽名夏麿, 桧垣匠, 永田典子, 櫻井哲也, 松岡健, 持田恵一, 佐藤繭子
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都,京都産業大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Electron microscopy atlas : ultrastructure and distribution of vesicle cluster2012

    • Author(s)
      Toyooka K, Sato M, Kutsuna N, Higaki T, Nagata N, Mochida K, Matsuoka K, Shinozaki K
    • Organizer
      The 1st International Symposium on Plant Environmental Sensing
    • Place of Presentation
      京都,東大寺総合文化センター
    • Related Report
      2013 Final Research Report
  • [Presentation] 透過電子顕微鏡における広域高解像度画像取得法の開発とその応用2012

    • Author(s)
      澤木史江, 小林恵, 佐藤繭子, 朽名夏麿, 桧垣匠, 馳澤盛一郎, 豊岡公徳, 永田典子
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫,兵庫県立大学
    • Related Report
      2013 Final Research Report
  • [Presentation] TEM画像、600枚つなげてみました2011

    • Author(s)
      永田典子
    • Organizer
      植物電子顕微鏡若手ワークショップ2011
    • Place of Presentation
      横浜,理化学研究所
    • Year and Date
      2011-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 花粉の美しい模様はどのようにして作られるのか2011

    • Author(s)
      永田典子
    • Organizer
      日本女子大学バイオイメージングセンターシンポジウム「形の不思議」
    • Place of Presentation
      東京, 日本女子大学
    • Related Report
      2011 Research-status Report
  • [Book] The selective increase or decrease of organelle DNAs in young generative cells controls cytoplasmic inheritance in higher plants."Atlas of Plant Cell Structure"2014

    • Author(s)
      Nagata N
    • Publisher
      Springer(in press)
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://mcm-www.jwu.ac.jp/~n-nagata/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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