Isolation and characterization of ubiquitin-like protein MNSF-conjugating enzymes

• Project/Area Number: 23570138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Shimane University
• Principal Investigator: NAKAMURA Morihiko 島根大学, 産学連携センター, 教授 (20155865)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ユビキチン様タンパク質 / 結合酵素 / 翻訳後修飾 / ユビキチン類似タンパク質 / タンパク質翻訳後修飾

Research Abstract

In this study, we identified and characterized an enzyme which conjugates MNSF to target protein(s). This enzyme is a novel protein similar to ubiquitin conjugating enzyme E3. In addition, we also identified a heat shock protein which stabilizes aggregative MNSF. Thus, the mechanism of MNSF conjugation was partly shown.

Research Products

• [Journal Article] Ubiquitin-like protein MNSFβnegatively regulates T cell function and survival (2014)
  • Author(s): Nakamura M, Nakagawa M, Watanabe J.
  • Journal Title: Immunol Invest Volume: (in press)
  • Peer Reviewed
• [Journal Article] Ubiquitin-like protein MNSFβ negatively regulates T cell function and survival (2014)
  • Author(s): Nakamura M, Nakagawa M
  • Journal Title: Immunological Investigations Volume: -
  • Peer Reviewed
• [Journal Article] Ubiquitin-like protein MNSFβcovalently binds to Bcl-G and enhances lipopolysaccharide (LPS)/interferonγ(IFNγ)-induced apoptosis in macrophages (2013)
  • Author(s): Wtanabe J, Nakagawa M, Nakamura M.
  • Journal Title: FEBS J. Volume: 280 Pages: 1281-93
  • Peer Reviewed
• [Journal Article] Preliminary study on using accelerometers to measure involuntary movement for the assessment of neurological motor impairments (2013)
  • Author(s): Iwasaki Y, Hirotomi T, Oguro H, Nakamura M.
  • Journal Title: Advanced Applied Informatics Volume: 46 Pages: 33-38
  • Peer Reviewed
• [Journal Article] Ubiquitin-like protein MNSFβ covalently binds to Bcl-G and enhances lipopolysaccharide/interferonγ-induced apoptosis in macrophages. (2013)
  • Author(s): Watanabe J, Nakagawa M, Watanabe N, Nakamura M
  • Journal Title: FEBS J Volume: 280 Issue: 5 Pages: 1281-1293
  • DOI: 10.1111/febs.12120
  • Peer Reviewed
• [Journal Article] Ubiquitin-like protein MNSFβregulates TLR-2-mediated signal transduction (2012)
  • Author(s): Nakamura M, Watanabe J, Watanabe N.
  • Journal Title: Mol Cell Biochem Volume: 364 Pages: 39-43
  • Peer Reviewed
• [Journal Article] New surface-modified zinc oxide nanoparticles with aminotriethylene oxide chains linked by 1,2,3-triazole ring : preparation, and visible light-emitting and noncytotoxic properties (2012)
  • Author(s): Sato M, Shimatani K, Iwasaki Y, Morito S., Tanaka H, Fujita Y, Nakamura M.
  • Journal Title: Applied Surface Science Volume: 258 Pages: 786-790
  • Peer Reviewed
• [Journal Article] Chronic administration of cardanol (Ginkgol) extracted from Ginkgo biloba leaves and cashew nutshell liquid improves Working Memory-Related learning in rats (2012)
  • Author(s): Tobinaga S, Hashimoto M, Utsunomiya I, Taguchi K, Nakamura M, Tsunematsu T.
  • Journal Title: Biol Pharm Bull Volume: 35 Pages: 127-129
  • NAID: 130001872336
  • Peer Reviewed
• [Journal Article] Ubiquitin-like protein MNSFβ regulates TLR-2-mediated signal transduction. (2012)
  • Author(s): Nakamura M, Watanabe J, Watanabe N
  • Journal Title: Mol Cell Biochem Volume: 364 Issue: 1-2 Pages: 39-43
  • DOI: 10.1007/s11010-011-1202-x
  • Peer Reviewed
• [Journal Article] Chronic Administration of Cardanol (Ginkgol) Extracted from Ginkgo biloba Leaves and Cashew Nutshell Liquid Improves Working Memory-Related Learning in Rats (2012)
  • Author(s): Tobinaga S, Hashimoto M, Utsunomiya I, Taguchi K, Nakamura M, Tsunematsu T
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 35 Issue: 1 Pages: 127-129
  • DOI: 10.1248/bpb.35.127
  • NAID: 130001872336
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] New surface-modified zinc oxide nanoparticles with aminotriethylene oxide chains linked by 1,2,3-triazole ring: preparation, and visible light-emitting and noncytotoxic properties. (2012)
  • Author(s): Sato M, Shimatania K, Iwasakia Y, Moritoa S., Tanaka H, Fujita Y, Nakamura M
  • Journal Title: Applied Surface Science. Volume: in press Issue: 2 Pages: 786-790
  • DOI: 10.1016/j.apsusc.2011.08.096
  • Peer Reviewed
• [Journal Article] Anti-allergic effect of the flavonoid myricitrin from Myrica rubra leaf extracts in vitro and in vivo. (2011)
  • Author(s): Shimosaki S, Tsurunaga Y, Itamura H, Nakamura M
  • Journal Title: Natural Product Research Volume: 25 Issue: 4 Pages: 374-380
  • DOI: 10.1080/14786411003774320
  • Peer Reviewed
• [Presentation] Isolation and characterization of ubiquitin-like protein MNSFβconjugating enzymes (2013)
  • Author(s): Nakagawa M, Nakamura M
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-11
• [Presentation] Preliminary study on using accelerometers to measure involuntary movements for the assessment of neurological motor impairments (2013)
  • Author(s): Iwasaki Y, Hirotomi T, Oguro H, Nakamura M.
  • Organizer: Second IIAI International Conference on Advanced Applied Informatics Matsue
  • Place of Presentation: Japan
  • Year and Date: 2013-09-04
• [Presentation] Ubiquitin- like protein MNSFβcovalently binds to Bcl-G and enhances lipopolysaccharide (LPS)/interferonγ(IFNγ)-induced apoptosis in macrophages (2013)
  • Author(s): Nakamura M, Nakagawa M.
  • Organizer: 15^<th> International Congress of Immunology Milan
  • Place of Presentation: Italy
  • Year and Date: 2013-08-24
• [Presentation] Usefulness of aromatherapy ("Shimane rose water") on bathing process for elderly with dementia living in a geriatric health service facility (2013)
  • Author(s): Takeda Y, Hara S, Hasegawa S, Ono M, Nakamura M.
  • Organizer: Alzheimer's Disease International (ADI)
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2013-04-20
• [Presentation] Isolation and characterization of ubiquitin-like protein MNSFβ conjugating enzymes. (2013)
  • Author(s): Nakagawa M, Nakamura M
  • Organizer: 日本生化学会
  • Place of Presentation: （ 横浜 ）横浜パシフィコ
• [Presentation] Ubiquitin-like protein MNSFβ covalently binds to Bcl-G and enhances lipopolysaccharide (LPS)/interferon γ (IFNγ)-induced apoptosis in macrophages. (2013)
  • Author(s): Nakamura M, Nakagawa M
  • Organizer: 15th International Congress of Immunology
  • Place of Presentation: （ Milan）Milano Congressi
• [Presentation] Usefulness of aromatherapy (“Shimane rose water”) on bathing process for elderly with dementia living in a geriatric health service facility. (2013)
  • Author(s): Takeda Y, Hara S, Hasegawa S, Ono M, Nakamura M
  • Organizer: Alzheimer’s Disease International
  • Place of Presentation: （ Taipei）The Taipei International Convention Centre
• [Presentation] Ubiquitin-like protein MNSFβregulates apoptosis through ERK/ AP-1 pathway in LPS/IFNγ- stimulated macrophages (2012)
  • Author(s): Watanabe J, Nakagawa M, Nakamura M.
  • Organizer: 第85回日本生化学会
  • Place of Presentation: 博多
  • Year and Date: 2012-12-14
• [Presentation] Ubiquitin-like Protein MNSFβ Regulates Apoptosis through ERK/AP-1 Pathway in LPS/IFNγ-stimulated Macrophages (2012)
  • Author(s): Watanabe J, Nakagawa M, Nakamura M
  • Organizer: 第85回 日本生化学会
  • Place of Presentation: 福岡国際会議場（ 福岡市）
• [Presentation] Ubiquitin-like protein MNSFβenhances LPS/IFNγ- induced apoptosis in macrophages (2011)
  • Author(s): Watanabe J, Watanabe N, Nakamura M.
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] Ubiquitin-like protein MNSFβenhances LPS/IFNγ-induced apoptosis in macrophages.
  • Author(s): Watanabe J, Watanabe N, Nakamura M
  • Organizer: 第84回 日本生化学会
  • Place of Presentation: 国立京都国際会館（京都市）
• [Remarks]
  • URL: http://www.ed.shiane-u.ac.jp/CMRC/index2.html
• [Remarks] 島根大学産学連携センター地域医学共同研究部門
  • URL: http://www.med.shimane-u.ac.jp/CMRC/index2.htm
• [Patent(Industrial Property Rights)] 水溶性超常磁性ナノ粒子 (2013)
  • Inventor(s): 佐藤守之, 中村守彦
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-01-09
  • Overseas
• [Patent(Industrial Property Rights)] 蛍光標識材料および蛍光標識剤 (2013)
  • Inventor(s): 佐藤守之, 中村守彦
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2013-08-02
  • Overseas
• [Patent(Industrial Property Rights)] Fluorescent labeling material and fluorescent labeling agent (2013)
  • Inventor(s): Sato M, Nakamura M.
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2013-09-25
  • Overseas
• [Patent(Industrial Property Rights)] 水溶性超常磁性ナノ粒子 (2013)
  • Inventor(s): 佐藤守之、中村守彦
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-002041
  • Filing Date: 2013-01-09
• [Patent(Industrial Property Rights)] 情報ネットワークシステム (2012)
  • Inventor(s): 橋口尚幸, 中村守彦, 四郎丸功, 増成博志
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-01-25
  • Overseas