Structural analysis of the regulation of mitotic spindle orientation by the cell polarity protein LGN

• Project/Area Number: 23570141
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: YUZAWA Satoru 九州大学, 医学(系)研究科(研究院), 助教 (40515029)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞極性 / 細胞内シグナル伝達 / X線結晶構造解析 / 分子認識 / シグナル伝達 / タンパク質間相互作用 / モチーフ / 分子内相互作用 / 自己阻害状態 / 蛋白質間相互作用 / TPRモチーフ / 細胞極性の形成 / LGN

Research Abstract

Asymmetric cell division generates cell type diversity and is crucial for the development and tissue organization. The interactions between the cell polarity protein mInsc and LGN regulate mitotic spindle orientation during asymmetric cell division in certain types of cells. The complex of mInsc and LGN is targeted to plasma membrane and form complexes with other proteins. In this project, we have determined the crystal structure of LGN in complex with mInsc and elucidate the molecular mechanism of LGN regulated by its intramolecular interaction.

Research Products

• [Journal Article] Ubiquitination of the heterotrimeric G protein α subunits Gα i2 and Gαq is prevented by the guanine nucleotide exchange factor Ric-8A (2013)
  • Author(s): Kanako Chishiki, Sachiko Kamakura, Satoru Yuzawa, Junya Hayase, Hideki Sumimoto.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 435 Pages: 414-419
  • Peer Reviewed
• [Journal Article] Ubiquitination of the heterotrimeric G protein α subunits Gαi2 and Gαq is prevented by the guanine nucleotide exchange factor Ric-8A (2013)
  • Author(s): Chishiki, K., Kamakura, S., Yuzawa, S., Hayase, J., Sumimoto, H.
  • Journal Title: Biochem Biophys Res Commun Volume: 435 Issue: 3 Pages: 414-419
  • DOI: 10.1016/j.bbrc.2013.04.103
  • Peer Reviewed
• [Journal Article] 細胞極性タンパク質LGN とパートナー分子mInsc 複合体の認識機構 (2012)
  • Author(s): 湯澤 聡
  • Journal Title: 日本結晶学会誌 Volume: 54 Pages: 206-212
  • NAID: 130002142912
  • Peer Reviewed
• [Journal Article] Crystal structure of the C-terminal globular domain of oligosaccharyltransferase from Archaeoglobus fulgidus at 1.75 Å resolution (2012)
  • Author(s): Shunsuke Matsumoto, Mayumi Igura, James Nyirenda, Masaki Matsumoto, Satoru Yuzawa, Nobuo Noda, Fuyuhiko Inagaki, Daisuke Kohda.
  • Journal Title: Biochemistry Volume: 51 Pages: 4157-4166
  • Peer Reviewed
• [Journal Article] 細胞極性タンパク質LGNとパートナー分子mInsc複合体の認識機構 (2012)
  • Author(s): 湯澤聡
  • Journal Title: 日本結晶学会誌 Volume: 54 Pages: 206-212
  • NAID: 130002142912
  • Peer Reviewed
• [Journal Article] Structural basis for interaction between the conserved cell polarity proteins Inscuteable and Leu-Gly-Asn repeat-enriched protein (LGN) (2011)
  • Author(s): Satoru Yuzawa, Sachiko Kamakura, Yuko Iwakiri, Junya Hayase, Hideki Sumimoto.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 19210-19215
  • Peer Reviewed
• [Presentation] 細胞極性タンパク質LGN のTPR ドメインによるパートナー分子の多様な認識 (2012)
  • Author(s): 湯澤聡,鎌倉幸子,岩切優子,早瀬純也,住本英樹
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 細胞極性タンパク質LGNのTPRドメインによるパートナー分子の多様な認識 (2012)
  • Author(s): 湯澤 聡，鎌倉 幸子，岩切 優子，早瀬 純也，住本 英樹
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 細胞極性関連タンパク質 Inscuteable とLGN との複合体のX 線結晶構造解析 (2011)
  • Author(s): 湯澤聡,鎌倉幸子,岩切優子,早瀬純也,住本英樹
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] 細胞極性関連タンパク質InscuteableとLGNとの複合体のX線結晶構造解析 (2011)
  • Author(s): 湯澤 聡，鎌倉幸子，岩切優子，早瀬純也，住本英樹
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜