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Analysis of erythroid differentiation by HSP27

Research Project

Project/Area Number 23570172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionKeio University

Principal Investigator

KABE Yasuaki  慶應義塾大学, 医学部, 講師 (20397037)

Co-Investigator(Renkei-kenkyūsha) ISHIMORI Koichiro  北海道大学, 理学部, 教授 (20192487)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsヘム / 赤血球分化 / 金属タンパク質 / アポトーシス
Research Abstract

During erythropoiesis, haem synthesis is significantly induced, and abundant haem is utilized as hemoglobin in erythroid cells. However, excessive haem results in cell damages by membrane oxidation. In the present study, we found that haem directly bound to heat shock protein 27 (HSP27) by affinity purification. HSP27 is a small heat shock family protein, which plays an important role for cytoprotection, stress tolerance, cellular differentiation or tissue development. It has been known that the multimerized HSP27 dissociates by several stimuli. Interestingly, exposure of haem directly dissociated the multimerized HSP27 in vivo and in vitro. Knockdown of HSP27 expression resulted in the haem-induced apoptosis and reduced the haemin-induced erythroid differentiation. Furthermore, we also found that HSP27 is essential for erythroid differentiation by using HSP27 knockdown mice.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (39 results)

All 2014 2013 2012 2011

All Journal Article (19 results) (of which Peer Reviewed: 18 results) Presentation (12 results) (of which Invited: 2 results) Book (6 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Microscopic imaging mass spectrometry assisted by on-tissue chemical derivatization for visualizing multiple amino acids in human colon cancer xenografts.2014

    • Author(s)
      Toue S, Sugiura Y, Kubo A, Ohmura M, Karakawa S, Mizukoshi T, Yoneda J, Miyano H, Noguchi Y, Kobayshi T, Kabe Y, Suematsu M.
    • Journal Title

      Proteomics

      Volume: 14 Issue: 7-8 Pages: 810-819

    • DOI

      10.1002/pmic.201300041

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microscopic imaging mass spectrometry assisted by on-tissue chemical derivatization for visualizing multiple amino acids in human colon cancer xenografts2013

    • Author(s)
      Toue S, Sugiura Y, Kubo A, Ohmura M, Karakawa S, Mizukoshi T, Yoneda J, Miyano H, Noguchi Y, Kobayshi T, Kabe Y, Suematsu M.
    • Journal Title

      Proteomics

      Volume: 14(7-8) Pages: 810-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vesnarinone suppresses TNFαmRNA expression by inhibiting valosin-containing protein2013

    • Author(s)
      Hotta K, Nashimoto A, Yasumura E, Suzuki M, Azuma M, Iizumi Y, Shima D, Nabeshima R, Hiramoto M, Okada A, Sakata-Sogawa K, Tokunaga M, Ito T, Ando H, Sakamoto S, Kabe Y, Aizawa S, Imai T, Yamaguchi Y, Watanabe H, Handa H.
    • Journal Title

      Mol Pharmacol

      Volume: 83(5) Pages: 930-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vitamin K2 covalently binds to Bak and induces Bak-mediated apoptosis2013

    • Author(s)
      Karasawa S, Azuma M, Kasama T, Sakamoto S, Kabe Y, Imai T, Yamaguchi Y, Miyazawa K, Handa H.
    • Journal Title

      Mol Pharmacol

      Volume: 83(3) Pages: 613-20

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vesnarinone Suppresses TNF α mRNA Expression by Inhibiting Valosin-containing Protein2013

    • Author(s)
      Hotta K, Nashimoto A, Yasumura E, Suzuki M, Azuma M, Iizumi Y, Shima D, Nabeshima R, Hiramoto M, Okada A, Sakata-Sogawa K, Tokunaga M, Ito T, Ando H, Sakamoto S, Kabe Y, Aizawa S, Imai T, Yamaguchi Y, Watanabe H, Handa H
    • Journal Title

      Mol Pharmacol

      Volume: Vol.83 Issue: 5 Pages: 930-938

    • DOI

      10.1124/mol.112.081935

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vitamin K2 covalently binds to Bak and induces Bak-mediated apoptosis.2013

    • Author(s)
      Karasawa S, Azuma M, Kasama T, Sakamoto S, Kabe Y, Imai T, Yamaguchi Y, Miyazawa K, Handa H
    • Journal Title

      Mol Pharmacol

      Volume: 83 Issue: 3 Pages: 613-620

    • DOI

      10.1124/mol.112.082602

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microscopic imaging mass spectrometry assisted by on-tissue chemical derivatization for visualizing multiple amino acids in human colon cancer xenografts2013

    • Author(s)
      Toue S, Sugiura Y, Kubo A, Ohmura M, Karasawa S, Mizukoshi M, Yoneda J, MIyano H, Noguchi Y, KObayashi T, Kabe Y, Suematsu M
    • Journal Title

      Proteomics

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Vesnarinone Suppresses TNFα mRNA Expression by Inhibiting Valosin-containing Protein.2013

    • Author(s)
      Hotta K, Nashimoto A, Yasumura E, Suzuki M, Azuma M, Iizumi Y, Shima D, Ando H, Sakamoto S, Kabe Y, Aizawa S, Imai T, Yamaguchi Y, Watanabe H, Handa H.
    • Journal Title

      Mol Pharmacol.

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of NF-κB-mediated expression of galectin-3-binding protein in TNF-α-induced breast cancer cell adhesion2012

    • Author(s)
      Noma N, Simizu S, Kambayashi Y, Kabe Y, Suematsu M, Umezawa K.
    • Journal Title

      Oncol Rep

      Volume: 27(6) Pages: 2080-4

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway2012

    • Author(s)
      Morikawa T, Kajimura M, Nakamura T, Hishiki T, Nakanishi T, Yukutake Y, Nagahata Y, Ishikawa M, Hattori K, Takenouchi T, Takahashi T, Ishii I, Matsubara K, Kabe Y, Uchiyama S, Nagata E, Gadalla MM, Snyder SH, Suematsu M.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 109(4) Pages: 1293-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] HIF-1αinduction suppresses excessive lipid accumulation in alcoholic fatty liver in mice2012

    • Author(s)
      Nishiyama Y, Goda N, Kanai M, Niwa D, Osanai K, Yamamoto Y, Senoo-Matsuda N, Johnson RS, Miura S, Kabe Y, Suematsu M.
    • Journal Title

      J Hepatol.

      Volume: 56(2) Pages: 441-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of NF-κB-mediated expression of galectin-3-binding protein in TNF-α-induced breast cancer cell adhesion.2012

    • Author(s)
      Noma N, Simizu S, Kambayashi Y, Kabe Y, Suematsu M, Umezawa K.
    • Journal Title

      Oncol Rep.

      Volume: 27 Pages: 2080-2084

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway.2012

    • Author(s)
      Morikawa T, Kajimura M, Nakamura T, Hishiki T,Ishikawa M, Hattori K, Takenouchi T, Takahashi T, Ishii I, Matsubara K, Kabe Y, Uchiyama S, Nagata E, Gadalla MM, Snyder SH, Suematsu M.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 109 Pages: 1293-1298

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] HIF-1α induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice.2012

    • Author(s)
      Nishiyama Y, Goda N, Kanai M, Niwa D, Osanai K, Yamamoto Y, Senoo-Matsuda N, Johnson RS, Miura S, Kabe Y, Suematsu M.
    • Journal Title

      J Hepatol.

      Volume: 56 Pages: 441-447

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of NF-κB-mediated expression of galectin-3-binding protein in TNF-α-induced breast cancer cell adhesion.2012

    • Author(s)
      Noma N, Simizu S, Kambayashi Y, Kabe Y, Suematsu M, Umezawa K
    • Journal Title

      Oncology Reports

      Volume: 27 Pages: 2080-2084

    • URL

      http://www.spandidos-publications.com/serveFile/or_27_6_2080_PDF.pdf?type=article&article_id=or_27_6_2080&item=PDF

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of dynamin-2-mediated endocytosis as a new target of osteoporosis drugs, bisphosphonates2011

    • Author(s)
      Masaike Y, Takagi T, Hirota M, Yamada J, Ishihara S, Yung TM, Inoue T, Sawa C, Sagara H, Sakamoto S, Kabe Y, Takahashi Y, Yamaguchi Y, Handa H.
    • Journal Title

      Mol Pharmacol.

      Volume: 77(2) Pages: 262-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gas Biology Research in Clinical Practice : Roles of Stress-Inducible Carbon Monoxide in the Regulation of Liver Function2011

    • Author(s)
      Suematsu M, Kajimura M, Kabe Y.
    • Journal Title

      KARGAR press

      Pages: 1-5

    • Related Report
      2013 Final Research Report
  • [Journal Article] Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway.2011

    • Author(s)
      Morikawa T, Kajimura M, Nakamura T, Kabe Y, et al
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 109 Pages: 1293-1298

    • URL

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268316/pdf/pnas.201119658.pdf

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] HIF-1α induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice.2011

    • Author(s)
      Nishiyama Y, Goda N, Kanai M, Niwa D, Osanai K, Yamamoto Y, Senoo-Matsuda N, Johnson RS, Miura S, Kabe Y, Suematsu M.
    • Journal Title

      Journal of Hepatology

      Volume: 56 Pages: 441-447

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 新規 CO ガス受容体を介した肝障害制御機構の解明2014

    • Author(s)
      加部泰明
    • Organizer
      第12回分子予防環境医学研究会
    • Place of Presentation
      つくばサイエンス・インフォメーションセンター
    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Invited
  • [Presentation] 新規COガス受容体を介した肝障害制御機構の解明2013

    • Author(s)
      加部泰明
    • Organizer
      第12回分子予防環境医学研究会
    • Place of Presentation
      つくばサイエンス・インフォメーションセンター
    • Year and Date
      2013-02-01
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ヘム/COによるGAPDHを介した細胞防御機構の解明2012

    • Author(s)
      加部泰明, 末松誠
    • Organizer
      第35回日本分子生物学会年回
    • Place of Presentation
      福岡国際会議場 (口頭・ポスター発表)
    • Year and Date
      2012-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of the GAPDH-mediated cell death by CO gas2012

    • Author(s)
      加部泰明、半田宏、末松誠
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] COガスによる細胞防御機構の解明2011

    • Author(s)
      加部泰明, 末松誠
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜 (口頭・ポスター発表)
    • Year and Date
      2011-12-15
    • Related Report
      2013 Final Research Report
  • [Presentation] ガス分子応答性受容体の系統的探索と機能解明2011

    • Author(s)
      加部泰明, 末松誠
    • Organizer
      第64回日本酸化ストレス学会
    • Place of Presentation
      北海道ルスツリゾートホテル&コンベンション (口頭発表)
    • Year and Date
      2011-07-03
    • Related Report
      2013 Final Research Report
  • [Presentation] ヘム/COによるGAPDHを介した細胞防御機構の解明2011

    • Author(s)
      加部泰明, 末松誠
    • Organizer
      第38回生体分子科学討論会
    • Place of Presentation
      筑波大学大学会館国際会議室 口頭発表)
    • Year and Date
      2011-06-23
    • Related Report
      2013 Final Research Report
  • [Presentation] ヘム/COによる細胞防御機構の解明2011

    • Author(s)
      加部泰明, 末松誠
    • Organizer
      第32回日本炎症・再生医学会
    • Place of Presentation
      国立京都国際会館 口頭発表)
    • Year and Date
      2011-06-03
    • Related Report
      2013 Final Research Report
  • [Presentation] ヘム/COによるGAPDHを介した細胞防御機構の解明2011

    • Author(s)
      加部 泰明、半田 宏、末松 誠
    • Organizer
      日本ケミカルバイオロジー学会 第6回年会
    • Place of Presentation
      東京工業大学大岡山キャンパス(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] ヘム/COによるGAPDHを介した細胞防御機構の解明2011

    • Author(s)
      加部泰明、内田毅、山本竜也、石森浩一郎、半田宏、末松誠
    • Organizer
      第38回生体分子科学討論会
    • Place of Presentation
      筑波大学(茨城県)
    • Related Report
      2011 Research-status Report
  • [Presentation] ガス分子応答性受容体の系統的探索機能解明2011

    • Author(s)
      加部泰明、内田毅、山本竜也、堀内正、石森浩一郎、半田宏、末松誠
    • Organizer
      第64回日本酸化ストレス学会
    • Place of Presentation
      北海道ルスツリゾートホテル(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Carbon Monoxide Protects Against the GAPDH-mediated Cell Death2011

    • Author(s)
      加部泰明、山本竜也、半田宏、末松誠
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Book] 実験医学別冊「医学生物学における最新プロトコールと実験例」2013

    • Author(s)
      加部泰明, 末松誠, 半田宏
    • Publisher
      羊土社
    • Related Report
      2013 Final Research Report
  • [Book] 実験医学別冊「医学生物学における最新プロトコールと実験例」2013

    • Author(s)
      加部泰明、末松誠、半田宏
    • Publisher
      羊土社
    • Related Report
      2013 Annual Research Report
  • [Book] 実験医学別冊、質量分析実験ガイド2013

    • Author(s)
      加部泰明、末松誠、半田宏
    • Publisher
      リガンド結合タンパク質を見つける
    • Related Report
      2012 Research-status Report
  • [Book] 臨床麻酔「ガス分子による代謝システム制御機構の系統的探索と応用」2012

    • Author(s)
      末松誠, 梶村真弓, 加部泰明.
    • Publisher
      真興交易医書
    • Related Report
      2013 Final Research Report
  • [Book] 実験医学「がんと代謝」2012

    • Author(s)
      末松誠, 久保亜紀子, 大村光代, 菱木貴子, 梶村真弓, 加部泰明, 杉浦悠基
    • Publisher
      羊土社
    • Related Report
      2013 Final Research Report
  • [Book] 実験医学増刊「in vivo実験医学によるヒト疾患解明の最前線」2011

    • Author(s)
      末松誠, 梶村真弓, 加部泰明, 山本雄広
    • Publisher
      羊土社
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 一酸化炭素(CO)による脂肪酸、 コレステロールの取り込み阻害2011

    • Inventor(s)
      加部泰明, 末松誠
    • Industrial Property Rights Holder
      加部泰明, 末松誠
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2011-263015
    • Filing Date
      2011-11-17
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 一酸化炭素(CO)による脂肪酸、コレステロールの取り込み阻害2011

    • Inventor(s)
      加部泰明、末松誠
    • Industrial Property Rights Holder
      加部泰明、末松誠
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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