• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Fibrin translocation to platelet membrane rafts in blood coagulation

Research Project

Project/Area Number 23570182
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

KASAHARA Kohji  公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 副室長 (60250213)

Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsラフト / 血小板 / フィブリン / 血液凝固
Outline of Final Research Achievements

We observed that sphingomyelin (SM)-rich rafts are identified histochemically in the central region of adhered platelets where fibrin is colocalized on activation by thrombin. The clot retraction of SM-depleted platelets from SM synthase knockout mouse was delayed significantly, suggesting that platelet SM-rich rafts are involved in clot retraction. Furthermore, fibrin translocation to DRM rafts was impaired in factor XIII A subunit-deficient mouse platelets, which show impaired clot retraction. Furthermore, the disruption of SM-rich rafts by methyl-β-cyclodextrin impaired clot retraction. Thus, we propose that clot retraction takes place in SM-rich rafts where a fibrin-αIIbβ3 complex is formed as a primary axis to promote platelet contraction.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (30 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (17 results) (of which Invited: 3 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Update Review血小板による血栓形成メカニズム研究の最新動向2014

    • Author(s)
      笠原浩二 山本正雅
    • Journal Title

      実験医学

      Volume: 32 Pages: 2966-2971

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fibrin-translocation to platelet lipid rafts and clot retraction2014

    • Author(s)
      Kasahara K
    • Journal Title

      Japanese Journal of Thrombosis and Hemostasis

      Volume: 25 Issue: 1 Pages: 92-94

    • DOI

      10.2491/jjsth.25.92

    • NAID

      130003393345

    • ISSN
      0915-7441, 1880-8808
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Introduce My Article2014

    • Author(s)
      笠原浩二
    • Journal Title

      Rinsho Ketsueki

      Volume: 55 Issue: 3 Pages: 366-367

    • DOI

      10.11406/rinketsu.55.366

    • NAID

      130004724859

    • ISSN
      0485-1439, 1882-0824
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clot retraction is mediated by factor XIII-dependent fibrin-aIIbb3-myosin axis in platelet sphingomyelin-rich membrane rafts2013

    • Author(s)
      Kasahara, K., Kaneda, M., Miki, T., Iida, K., Sekino-Suzuki, N., Kawashima, I., Suzuki, H., Shimonaka, M., Arai, M., Ohni-Iwashita, Y., Kojima, S., Abe, M., Kobayashi, T., Okazaki, T., Souri, M., Ichinose, A., Yamamoto, N.
    • Journal Title

      Blood

      Volume: 122(19) Issue: 19 Pages: 3340-3348

    • DOI

      10.1182/blood-2013-04-491290

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] An anti-sulfatide antibody O4 immunoprecipitates sulfatide rafts including Fyn, Lyn and the G protein alpha subunit in rat primary immature oligodendrocytes.2013

    • Author(s)
      Miki, T., Kaneda, M., Iida, K., Hasegawa, G., Murakami, M., Yamamoto, N., Asou, H., Kasahara, K.
    • Journal Title

      Glycoconj. J.

      Volume: 30(9) Issue: 9 Pages: 819-823

    • DOI

      10.1007/s10719-013-9487-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Change of dynamics of raft-model membrane induced by amyloid-beta protein binding2013

    • Author(s)
      Hirai, M., Kimura, R., Takeuchi, K., Sugiyama, M., Kasahara, K., Ohta, N., Farago, B., Stadler, A., Zaccai, G.
    • Journal Title

      Eur.Phys.J.E.Soft Matter

      Volume: 36(7) Issue: 7 Pages: 9887-9887

    • DOI

      10.1140/epje/i2013-13074-3

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of gangliosides in the process of Cbp/PAG phosphorylation by Lyn in developing cerebellar growth cones2013

    • Author(s)
      Sekino-Suzuki N, Yuyama K, Yamamoto T. (10名)
    • Journal Title

      J Neurochem

      Volume: Vol.124 Issue: 4 Pages: 514-522

    • DOI

      10.1111/jnc.12040

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel Bernard-Soulier syndrome variants caused by compound heterozygous mutations (case I) or a cytoplasmic tail truncation (case II) of GPIba.2013

    • Author(s)
      Yamamoto N, Akamatsu N, Sakuraba H, Matsuno K, Hosoya R, Nogami H, Kasahara K, Mituyama S, Arai M
    • Journal Title

      Thromb. Res.

      Volume: 131 Issue: 4 Pages: e160-e167

    • DOI

      10.1016/j.thromres.2013.01.014

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 三量体Gタンパク質Goaの糖脂質ミクロドメインにおけるシグナル伝達2012

    • Author(s)
      三木俊明、兼田瑞穂、*笠原浩二
    • Journal Title

      膜

      Volume: 37 Pages: 168-173

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ganglioside GD3 monoclonal antibody-induced paxillin tyrosine phosphorylation and filamentous actin assembly in cerebellar growth cones.2011

    • Author(s)
      Yuyama K, Sekino-Suzuki N, Yamamoto N, Kasahara K
    • Journal Title

      J.Neurochem.

      Volume: 116 Issue: 5 Pages: 845-850

    • DOI

      10.1111/j.1471-4159.2010.07071.x

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Clot retraction is mediated by factor XIII-dependent fibrin -alphaIIbbeta3 –myosin2014

    • Author(s)
      Kasahara K
    • Organizer
      BIT’s 5th World Gene Convention
    • Place of Presentation
      Haikou, China
    • Year and Date
      2014-11-13 – 2014-11-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] フィブリンの血小板脂質ラフト移行と血餅退縮2014

    • Author(s)
      笠原浩二
    • Organizer
      第19回近畿血栓症研究会
    • Place of Presentation
      北浜フォーラム(大阪市中央区)
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of neural raft signaling by anti-glycosphingolipid antibody.2014

    • Author(s)
      Kasahara K
    • Organizer
      International Symposium on Glyco-Neuroscience
    • Place of Presentation
      Awaji
    • Related Report
      2013 Research-status Report
  • [Presentation] Clot retraction is mediated by factor XIII-dependent fibrin-aIIbb3-myosin axis in platelet sphingomyelin-rich membrane rafts.2014

    • Author(s)
      Kasahara K, Ichinose A, Yamamoto N.
    • Organizer
      Keystone Symposia "New Frontier in the Discovery and Treatment of Thrombosis"
    • Place of Presentation
      Colorado, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Clot retraction is mediated by factor XIII-dependent fibrin-aIIbb3-myosin axis in platelet sphingomyelin-rich membrane rafts2014

    • Author(s)
      Kasahara K
    • Organizer
      58th Annual Meeting Society of Thrombosis and Haemostasis Research
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2013 Research-status Report
  • [Presentation] Involvement of Ganglioside GD3-raft Signaling in the Growth Cone Behavior of Cerebellar Granule Neurons2013

    • Author(s)
      Kasahara K
    • Organizer
      22th International Symposium on Glycoconjugates (Glyco22)
    • Place of Presentation
      Dalian, China
    • Related Report
      2013 Research-status Report
  • [Presentation] 神経系ラフトシグナルの抗糖脂質抗体による解析2013

    • Author(s)
      笠原浩二、兼田瑞穂、飯田和子、阿相皓晃、三木俊明
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] カルレティキュリンのプロテインジスルフィドイソメラーゼ活性の検討2013

    • Author(s)
      長谷川豪、斉藤佑尚、笠原浩二
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] 血液凝固XIII因子によるフィブリンの血小板スフィンゴミエリンラフト移行と血餅退縮2013

    • Author(s)
      笠原浩二
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] Fibrin-associated platelet rafts function as platform for clot retraction.2012

    • Author(s)
      Kasahara K
    • Organizer
      Gordon Research Conference “Glycolipid & Spingolipid Biology”
    • Place of Presentation
      イタリア
    • Related Report
      2012 Research-status Report
  • [Presentation] Translocation of fibrin and myosin into platelet membrane rafts is an important process for clot retraction via a functional property of GPIIb/IIIa.2011

    • Author(s)
      Kasahara K, Kaneda M, Miki T, Iida K, Suzuki H, Hara Y, Shimonaka M, Arai M, Kobayashi T, Ichinose A, Yamamoto N
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis.
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Presentation] 血小板の血液凝固第XIII因子基質としてのビンキュリン分解産物2011

    • Author(s)
      原裕太、兼田瑞穂、三木俊明、林もゆる、下仲基之、山本正雅、笠原浩二
    • Organizer
      日本生化学会関東支部例会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] Fibrin-integrin-myosin signaling axis in platelet membrane rafts2011

    • Author(s)
      Kasahara K
    • Organizer
      The 30th NAITO conference on membrane dymanics and lipid biology [II]
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] 糖脂質マイクロドメインを介するシグナル伝達による生体制御

    • Author(s)
      笠原浩二
    • Organizer
      日本膜学会第34年会生体膜シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 細胞膜ラフトにおける機能分子の集積とその意義

    • Author(s)
      笠原浩二
    • Organizer
      第27回日本動物細胞工学シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 血小板スフィンゴミエリン特異的ラフトへのフィブリン移行と血餅退縮

    • Author(s)
      笠原浩二、兼田瑞穂、三木俊明、飯田和子、小林俊秀、岡崎俊朗、山本正雅
    • Organizer
      第7回スフィンゴテラピイ研究会
    • Place of Presentation
      能登
    • Related Report
      2012 Research-status Report
  • [Presentation] 血小板スフィンゴミエリン特異的膜ラフトへのフィブリン移行と血餅退縮

    • Author(s)
      笠原浩二、兼田瑞穂、三木俊明、飯田和子、鈴木英紀、小林俊秀、岡崎俊朗、一瀬白帝、山本正雅
    • Organizer
      34回日本血栓止血学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Book] 実験医学2011

    • Author(s)
      笠原浩二
    • Total Pages
      3
    • Publisher
      Campus&Conference探訪記 ISTH2011 KYOTO JAPAN
    • Related Report
      2011 Research-status Report
  • [Remarks] 細胞膜研究室ホームページ

    • URL

      http://www.igakuken.or.jp/biomembrane/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京都医学総合研究所 細胞膜研究室

    • URL

      http://www.igakuken.or.jp/biomembrane/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi