Protein assembly at DNA replication fork and role of replication DNA helicase

• Project/Area Number: 23570204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Ibaraki University
• Principal Investigator: ISHIMI Yukio 茨城大学, 理学部, 教授 (80159772)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: MCMヘリカーゼ / DNA複製フォーク / タンパク質相互作用 / DNA複製 / タンパク質 / 酵素 / ゲノム / DNAヘリカーゼ / MCMタンパク質

Research Abstract

DNA replication fork progressed by MCM2-7 helicase is regulated by action of MCM interacting proteins. I clarified interaction of MCM2-7 with the MCM interacting proteins. CDC45 that is a component of CMG complex extensively interacted with all of MCM2-7. Similarly MCM-BP that is probably involved in inhibition of MCM2-7 complex interacted all of MCM2-7. This feature may be important for regulation of MCM function through structural change of MCM2-7 complex. Other MCM interactants bound to ATP binding domain of MCM6 and this binding may play a role in regulation of MCM function, since ATP binding to
MCM6 is critical for MCM function.

Research Products

• [Journal Article] Interaction of human minichromosome maintenance protein-binding protein with minichromosome maintenance 2-7 (2014)
  • Author(s): Kusunoki, S. and Ishimi, Y.
  • Journal Title: F EBS J. Volume: 281 Pages: 1057-1067
  • Peer Reviewed
• [Journal Article] Ｉｎｔｅｒａｃｔｉｏｎ ｏｆ human minichromosome maintenance protein-binding protein with minichromosome maintenance 2-7 (2014)
  • Author(s): Kusunoki, S. and Ishimi, Y.
  • Journal Title: FEBS J. Volume: 281 Issue: 4 Pages: 1057-1067
  • DOI: 10.1111/febs.12668
  • Peer Reviewed
• [Journal Article] Inhibition of DNA binding of MCM2-7 complex by phosphorylation with cyclin-dependent kinases (2013)
  • Author(s): Moritani, M. and Ishimi, Y.
  • Journal Title: J. Biochem. Volume: 154 Pages: 363-372
  • NAID: 40019817570
  • Peer Reviewed
• [Journal Article] Protein interaction and cellular localization of human CDC45 (2013)
  • Author(s): Takaya, J., Kusunoki, S. and Ishimi, Y.
  • Journal Title: J. Biochem. Volume: 153 Pages: 381-388
  • NAID: 40019622745
  • Peer Reviewed
• [Journal Article] Translocation and stability of replicative DNA helicases upon encountering DNA-protein cross-links (2013)
  • Author(s): Nakano, T., Miyamoto-Matsubara, M., Shoulkamy, M.I., Salem, A.M., Pack, S.P., Ishimi, Y. and Ide, H.
  • Journal Title: J. Biol. Chem. Volume: 288 Pages: 4649-4658
  • Peer Reviewed
• [Journal Article] Effect of an MCM4 mutation that causes tumours in mouse on human MCM4/6/7 complex formation (2012)
  • Author(s): Watanabe, E., Ohara, R. and Ishimi, Y.
  • Journal Title: J. Biochem. Volume: 152 Pages: 191-198
  • NAID: 10031166128
  • Peer Reviewed
• [Journal Article] Interaction of heliquinomycin with single-stranded DNA inhibits MCM4/6/7 helicase (2012)
  • Author(s): Sugiyama, T., Chino, M., Tsurimoto, T., Nozaki, N. and Ishimi, Y.
  • Journal Title: J. Biochem. Volume: 151 Pages: 129-137
  • NAID: 10031166051
  • Peer Reviewed
• [Journal Article] Binding of human MCM-BP with MCM2-7 proteins
  • Author(s): Kon, R., Kusunoki, S., Nozaki, N. and Ishimi, Y.
  • Journal Title: In DNA replication-Current advances Volume: (In Tech) Pages: 545-564
• [Presentation] MCM-BPとMCM2-7の相互作用 (2013)
  • Author(s): 楠俊輔, 石見幸男
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] CDK2/サイクリンAによるMCM2-7複合体のDNA結合阻害 (2013)
  • Author(s): 石見幸男, 守谷真梨子
  • Organizer: 日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-12
• [Presentation] CDK2/サイクリンAによるMCM2-7複合体のDNA結合阻害 (2013)
  • Author(s): 石見幸男, 守谷真梨子
  • Organizer: 日本生化学会関東支部例会
  • Place of Presentation: 山梨
  • Year and Date: 2013-06-15
• [Presentation] ヒトCDC45のタンパク質相互作用と細胞内局在 (2012)
  • Author(s): 高屋潤一郎, 野崎直仁, 石見幸男
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-13
• [Presentation] ヘリキノマイシンと一本鎖DNAとの相互作用によるMCM4/6/7ヘリカーゼの阻害 (2011)
  • Author(s): 杉山隆史, 千野信, 釣本俊樹, 野崎直仁, 石見幸男
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Interaction of heliquinomycin with single-stranded DNA inhibits MCM4/6/7 helicase (2011)
  • Author(s): Sugiyama, T., Chino, M., Tsurimoto, T., Nozaki, N. and Ishimi, Y.
  • Organizer: 第３４回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] CDK2/サイクリンAによるMCM2-7複合体のDNA結合阻害
  • Author(s): 石見幸男、守谷真梨子
  • Organizer: 日本生化学会
  • Place of Presentation: 横浜
• [Presentation] MCM-BPとMCM2-7の相互作用
  • Author(s): 楠俊輔、石見幸男
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Protein interaction and cellular localization of human CDC45
  • Author(s): Takaya, J ., Nozaki, N. and Ishimi, Y.
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Book] Binding of human MCM-BP with MCM2-7 proteins. in DNA replication-current advances (2011)
  • Author(s): Kon, R., Kusunoki, S., Nozaki, N. and Ishimi, Y.
  • Publisher: In Tech.
• [Remarks]
  • URL: http://idna.sci.ibaraki.ac.jp/