Protein assembly at DNA replication fork and role of replication DNA helicase
| Project/Area Number |
23570204
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Ibaraki University |
|---|
Principal Investigator |
ISHIMI Yukio 茨城大学, 理学部, 教授 (80159772)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | MCMヘリカーゼ / DNA複製フォーク / タンパク質相互作用 / DNA複製 / タンパク質 / 酵素 / ゲノム / DNAヘリカーゼ / MCMタンパク質 |
|---|
| Research Abstract |
DNA replication fork progressed by MCM2-7 helicase is regulated by action of MCM interacting proteins. I clarified interaction of MCM2-7 with the MCM interacting proteins. CDC45 that is a component of CMG complex extensively interacted with all of MCM2-7. Similarly MCM-BP that is probably involved in inhibition of MCM2-7 complex interacted all of MCM2-7. This feature may be important for regulation of MCM function through structural change of MCM2-7 complex. Other MCM interactants bound to ATP binding domain of MCM6 and this binding may play a role in regulation of MCM function, since ATP binding to
MCM6 is critical for MCM function.
|
Report
(4 results)
Research Products
(19 results)
-
-
-
-
-
[Journal Article] Translocation and stability of replicative DNA helicases upon encountering DNA-protein cross-links2013
Author(s)
Nakano, T., Miyamoto-Matsubara, M., Shoulkamy, M.I., Salem, A.M., Pack, S.P., Ishimi, Y. and Ide, H.
-
Journal Title
J. Biol. Chem.
Volume: 288
Pages: 4649-4658
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
