A study on the structure -function relationship of radial glia cells

• Project/Area Number: 23570226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: ZHU YAN 大阪大学, 生命機能研究科, 助教 (50464235)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 放射状グリア / 放射状突起 / CXCL12 / CXCR4 / β1インテグリン / 基底膜 / radial glia / radial processes / spinal cord / SDF-1 / Adhesion / Integrin / 放射状細胞 / マイクロ流体培養装置 / ケモカインSDF-1 / 子宮内細胞移植法 / 神経前駆細胞

Research Abstract

Radial glia cells are crucial to construct the nervous system. They have dual functions as neural progenitors as well as extending radial processes as physical scaffold for the developing neural tube. How their radial morphology is maintained and whether this is important are the key questions we have addressed in this project. Here we show that radial processes are disrupted in spinal cord of mouse mutants deficient in CXCL12/CXCR4/CXCR7 signaling. Interestingly this disruption causes leaky interface between the central nervous system and the periphery as cells from the latter are seen to invade into the former. We then showed that CXCL12 signaling affect the adhesiveness of radial glia to basement membrane components. Furthermore, we found that CXCL12 stimulation facilitates integrin B1 activation. Thus CXCL12/CXCR4/CXCR7 are important in maintaining the integrity of radial scaffold which in turn maintains the cellular segregation of the central nervous system.

Research Products

• [Journal Article] Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain (2013)
  • Author(s): Masaki Shinohara, Yan Zhu, Fujio Murakami
  • Journal Title: The Journal of comparative neurology Volume: 521 Issue: 14 Pages: 3340-57
  • DOI: 10.1002/cne.23353
  • Peer Reviewed
• [Journal Article] Four-Dimensional Analysis of Nucleogenesis of the Pontine Nucleus in the hindbrain (2013)
  • Author(s): Shinohara M, Zhu Y & Murakami F
  • Journal Title: Journal of Comparative Neurology Volume: in press
  • Peer Reviewed
• [Journal Article] Dynamics of the leading process, nucleus and Golgi apparatus of migrating cortical interneurons in living mouse embryos (2012)
  • Author(s): Yanagida M, Miyoshi R, Toyokuni R, Zhu Y & Murakami F
  • Journal Title: Proceedings of the National Academy of Sciences (PNAS) Volume: 109 Issue: 41 Pages: 16737-16742
  • DOI: 10.1073/pnas.1209166109
  • Peer Reviewed
• [Journal Article] Chemokine CXCL12 and its receptors in the developing central nervous system: emerging themes and future perspectives (2012)
  • Author(s): Zhu Y & Murakami F
  • Journal Title: Developmental Neurobiology Volume: 72 Issue: 10 Pages: 1349-1362
  • DOI: 10.1002/dneu.22041
  • Peer Reviewed
• [Presentation] A chemokine receptor CXCR7 non-cell-autonomously controls migration and nuclei formation of pontine neurons from the migratory environment (2011)
  • Author(s): Yan Zhu, Xun Hong, Koki Shirosaki, Frederic Sierro, Fabienne Mackay, Takashi Nagasawa, Fujio Murakami.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜会議場
  • Year and Date: 2011-09-15
• [Presentation] A chemokine receptor CXCR7 non-cell-autonomously controls migration and nuclei formation of pontine neurons from the migratory environment (2011)
  • Author(s): Yan Zhu
  • Organizer: The 34th annual meeting of the Japan Neuroscience Society 2011
  • Place of Presentation: Yokohama, Japan