Mechanism of the development of local organizers in the brain primordium using zebrafish as a model system

• Project/Area Number: 23570247
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Saitama University
• Principal Investigator: YAMASU Kyo 埼玉大学, 大学院理工学研究科, 教授 (60230439)
• Co-Investigator(Kenkyū-buntansha): KAWAMURA Akinori 埼玉大学, 大学院理工学研究科, 講師 (10466691)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脳形成 / 脳部域化 / 遺伝子制御ネットワーク / ゼブラフィッシュ / 発生遺伝学 / 局所オーガナイザー / 転写制御機構 / 神経分化 / 遺伝子ネットワーク / 転写制御 / エンハンサー解析 / 転写調節因子 / GAL4-UAS系 / マイクロアレイ / 遺伝子制御

Research Abstract

We studied the regulatory mechanism of vertebrate brain formation in zebrafish. We revealed the details of the function of two isthmus-forming genes, gbx2 and pou2, suggesting that both are involved in neurogenesis. We also extended the analysis on the transcriptional regulation of an ANB-forming gene, emx3, and two isthmus-forming genes, pax2a and pou2. Meanwhile, it was shown that the development of the neural crest and somite is affected in a novel mutation, kanazutsi, and its causative gene encodes a globin-family protein. In addition, we succeeded in introducing two novel experimental techniques, the GAL4/UAS system and TALEN.

Research Products

• [Journal Article] Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner (2013)
  • Author(s): Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K.
  • Journal Title: Mechanisms of Development Volume: 130 Issue: 11-12 Pages: 532-552
  • DOI: 10.1016/j.mod.2013.07.004
  • Peer Reviewed
• [Journal Article] Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages (2012)
  • Author(s): Khan A, Nakamoto A, Okamoto S, Tai M, Nakayama Y, Kobayashi K, Kawamura A, Takeda H, Yamasu K.
  • Journal Title: Mechasmims of Development Volume: 129 Issue: 9-12 Pages: 219-235
  • DOI: 10.1016/j.mod.2012.07.007
  • Peer Reviewed
• [Journal Article] Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafish embryogenesis. (2012)
  • Author(s): Khan A, Nakamoto A, Tai M, Saito S, Nakayama Y, Kawamura A, Takeda H, Yamasu K.
  • Journal Title: Development, Growth & Differentiation Volume: 54 Issue: 7 Pages: 686-701
  • DOI: 10.1111/j.1440-169x.2012.01369.x
  • NAID: 10031136935
  • Peer Reviewed
• [Journal Article] Binding properties of thyroxine to nuclear extract from sea urchin larvae (2012)
  • Author(s): M Saito, K Yamasu, T Suyemitsu
  • Journal Title: Zool Volume: 29 Issue: 2 Pages: 79-82
  • DOI: 10.2108/zsj.29.79
  • NAID: 40019149484
  • Peer Reviewed
• [Journal Article] Retinoic acid-dependent establishment of positional information in the hindbrain was conserved during vertebrate evolution (2011)
  • Author(s): A Ishioka, T Jindo, T Kawanabe, K Hatta, M S Parvin, M Nikaido, Y Kuroyanagi, H Takeda, K Yamasu
  • Journal Title: Dev Biol Volume: 350 Issue: 1 Pages: 154-168
  • DOI: 10.1016/j.ydbio.2010.10.011
  • Peer Reviewed
• [Presentation] A novel mutation involved in the development neural crest cells and somites in zebrafish embryos (2013)
  • Author(s): K. Takahashi, K. Yamasu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] Gbx2 regulates neurogenesis in the cerebellum during early vertebrate development (2013)
  • Author(s): Y. Nakayama, K. Yamasu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] In vitro analysis of the transcriptional regulatory mechanism of zebrafish pou2 that encodes a class V POU transcription factor (2013)
  • Author(s): K Kobayashi, K Yamasu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-03
• [Presentation] Repeated utilization of Pou2, a class V POU-type transcription factor in zebrafish, in regulation of multiple aspects of early development (2013)
  • Author(s): A. Khan, K. Yamasu
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: Barcelona Spain
  • Year and Date: 2013-07-11
• [Presentation] A novel mutation involved in neural crest differentiation in zebrafish embryos (2013)
  • Author(s): K. Takahashi, K. Yamasu
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: Barcelona Spain
  • Year and Date: 2013-07-10
• [Presentation] Studies on the gene network with a focus on Gbx2 transcription factor at the midbrain-hindbrain boundary (2013)
  • Author(s): Y. Nakayama, K. Yamasu
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: Barcelona Spain
  • Year and Date: 2013-07-10
• [Presentation] Gbx2 regulates neurogenesis in the cerebellum during early vertebrate development (2013)
  • Author(s): Yukiko Nakayama, Kana Kobayashi, Zhe Wang, Kyo Yamasu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] A novel mutation involved in the development neural crest cells and somites in zebrafish embryos (2013)
  • Author(s): Kazuki Takahashi, Yuki Ito, Mami Yoshimura, Masataka Nikaido, Akinori Kawamura, Kyo Yamasu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] In vitro analysis of the transcriptional regulatory mechanism of zebrafish pou2 that encodes a class V POU transcription factor (2013)
  • Author(s): Kana Kobayashi, Alam Khan, Kyo Yamasu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] Transcriptional regulation of hes6 gene in presomitic mesoderm and tailbud in zebrafish (2013)
  • Author(s): Akinori Kawamura, Miki Hoshikawa, Kenji Nakajo, Hiroki Ovara, Daisuke Yokota, Shin-ichiHigashijima, Shinji Takada, Kyo Yamasu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] Comprehensive analysis of gbx2 target genes suggests that gbx2 functions as a transcriptional activator or a repressor depending on tissues and target genes (2013)
  • Author(s): Yukiko Nakayama, Zhe Wang, Kana Kobayashi, Kyo Yamasu
  • Organizer: 第19回 小型魚類研究会
  • Place of Presentation: 仙台市情報・産業プラザ（宮城県仙台市）
• [Presentation] Transcriptional regulatory mechanism of pou2 that controls a broad spectrum of zebrafish development (2013)
  • Author(s): Kana Kobayashi, Alam Khan, Mst. Shahnaj Parvin, Kyo Yamasu
  • Organizer: 第19回 小型魚類研究会
  • Place of Presentation: 仙台市情報・産業プラザ（宮城県仙台市）
• [Presentation] A novel mutation involved in the development of neural crest-derived tissues and organs in zebrafish embryos (2013)
  • Author(s): Yuki Ito, Kazuki Takahashi, Mami Yoshimura, Masataka Nikaido, Akinori Kawamura, Kyo Yamasu
  • Organizer: 第19回 小型魚類研究会
  • Place of Presentation: 仙台市情報・産業プラザ（宮城県仙台市）
• [Presentation] A cis-regulatory module downstream of hes6 gene controlled by T-box and Ets transcription factors drives the expression in presomitic mesoderm and tailbud of zebrafish embryos (2013)
  • Author(s): Akinori Kawamura, Miki Hoshikawa, Kenji Nakajo, Hiroki Ovara, Daisuke Yokota, Shin-ichi Higashijima, Shinji Takada, Kyo Yamasu
  • Organizer: 第19回 小型魚類研究会
  • Place of Presentation: 仙台市情報・産業プラザ（宮城県仙台市）
• [Presentation] Repeated utilization of Pou2, a class V POU-type transcription factor in zebrafish, in regulation of multiple aspects of early development (2013)
  • Author(s): Alam Khan, Andrew Nakamoto, Yukiko Nakayama, Kana Kobayashi, Akinori Kawamura, Hiroyuki Takeda, and Kyo Yamasu
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: Palau de Congressos de Catalunya (Barcelona, Spain)
• [Presentation] Studies on the gene network with a focus on Gbx2 transcription factor at the midbrain-hindbrain boundary (2013)
  • Author(s): Yukiko Nakayama, Zhe Wang, Kana Kobayashi, Hiroshi Kikuta, Kyo Yamasu
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: Palau de Congressos de Catalunya (Barcelona, Spain)
• [Presentation] A novel mutation involved in neural crest differentiation in zebrafish embryos (2013)
  • Author(s): Yuki Ito, Kazuki Takahashi, Mami Yoshimura, Masataka Nikaido, Akinori Kawamura, Kyo Yamasu
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: Palau de Congressos de Catalunya (Barcelona, Spain)
• [Presentation] Functional analysis of the forebrain-forming genes in zebrafish embryos by the GAL4-UAS system (2012)
  • Author(s): H. Taira, K. Yamasu
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] Identification of the telencephalon enhancer of emx3 that functions in zebrafish embryos (2012)
  • Author(s): K. Shimura, K. Yamasu
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Functional analysis of zebrafish Gbx2 as a transcriptional regulator in embryos and cell culture system (2012)
  • Author(s): Y. Nakayama, K. Yamasu
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Functional analysis of Gbx2 in brain development using the heat shock induction system (2012)
  • Author(s): Y. Nakayama, K. Yamasu
  • Organizer: 10th International Conference on Zebrafish Development & Genetics
  • Place of Presentation: Madison USA
  • Year and Date: 2012-06-23
• [Presentation] Analysis of the roles played by a zebrafish class V POU protein Pou2/Pou5f1 in mesendodermal specification (2012)
  • Author(s): A. Khan, K. Yamasu
  • Organizer: 45th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Kobe
  • Year and Date: 2012-05-31
• [Presentation] The Gbx2 homeodomain protein functions as a transcriptional repressor in zebrafish embryos and embryonal carcinoma cells (2012)
  • Author(s): Y. Nakayama, K. Yamasu
  • Organizer: 45th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Kobe
  • Year and Date: 2012-05-29
• [Presentation] Analysis of the roles played by a zebrafish class V POU protein, Pou2/Pou5f1, in mesendodermal specification (2012)
  • Author(s): Alam Khan, Yukiko Nakayama & Kyo Yamasu
  • Organizer: 日本発生生物学会第45回大会
  • Place of Presentation: 神戸国際会議場（神戸）
• [Presentation] The Gbx2 homeodomain protein functions as a transcriptional repressor in zebrafish embryos and embryonal carcinoma cells (2012)
  • Author(s): Yukiko Nakayama, Kimihito Yoshikawa, Zhe Wang, Maiko Kanai, and Kyo Yamasu
  • Organizer: 日本発生生物学会第45回大会
  • Place of Presentation: 神戸国際会議場（神戸）
• [Presentation] Functional analysis of Gbx2 in brain development using the heat shock induction system (2012)
  • Author(s): Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta, Kyo Yamasu
  • Organizer: 10th International Conference on Zebrafish Development & Genetics
  • Place of Presentation: University of Wisconsin (Madison, USA)
• [Presentation] Functional analysis of the forebrain-forming genes in zebrafish embryos by the Gal4-UAS system (2012)
  • Author(s): Hitoshi Taira, Yuri Kuroyanagi, Koichi Kawakami, and Kyo Yamasu
  • Organizer: 第18回 小型魚類研究会
  • Place of Presentation: 京都大学（京都）
• [Presentation] Gbx2 functions as a transcriptional activator in zebrafish embryos (2012)
  • Author(s): Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta, Kyo Yamasu
  • Organizer: 第18回 小型魚類研究会
  • Place of Presentation: 京都大学（京都）
• [Presentation] 脊椎動物胚における脳形成制御機構の研究：発生遺伝学的アプローチ (2012)
  • Author(s): 弥益 恭
  • Organizer: 第３回 埼玉大学脳科学融合研究センター・シンポジウム「脳の発生・発達とその破綻の理解をめざして」
  • Place of Presentation: 埼玉大学（さいたま）
  • Invited
• [Presentation] Functional analysis of zebrafish Gbx2 as a transcriptional regulator in embryos and cell culture system (2012)
  • Author(s): Yukiko Nakayama, Zhe Wang, Kana Kobayashi, Hiroshi Kikuta, Maiko Kanai, and Kyo Yamasu
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] Identification of the telencephalon enhancer of emx3 that functions in zebrafish embryos (2012)
  • Author(s): Kyosuke Shimura, Yuri Kuroyanagi, Rieko Nakamura, Kyo Yamasu
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] Functional analysis of the forebrain-forming genes in zebrafish embryos by the GAL4-UAS system (2012)
  • Author(s): Hitoshi Taira, Ayano Shinoto, Gembu Abe, Koichi Kawakami, and Kyo Yamasu
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] 脊椎動物の発生において脳原基の部域化を制御する遺伝子ネットワーク (2012)
  • Author(s): 弥益 恭
  • Organizer: 埼玉大学・国立環境研究所共催シンポジウム「我々を取り巻く環境と健康 ～環境科学と基礎生物学の最近の研究動向～」
  • Place of Presentation: 埼玉会館（さいたま）
  • Invited
• [Presentation] Comprehensive analysis of the regulatory gene network initiated by gbx2 at the midbrain-hindbrain boundary (2011)
  • Author(s): Y. Nakayama, K. Yamasu
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Requirements of a Class V POU transcription factor Pou2/Pou5f1 in different aspects of zebrafish development (2011)
  • Author(s): A. Khan, K. Yamasu
  • Organizer: The 5th Asia-Oceania Zebrafish Meeting
  • Place of Presentation: Beijing China
  • Year and Date: 2011-08-26
• [Presentation] Functional alteration of the Gbx2 transcription factor during zebrafish brain development (2011)
  • Author(s): Y. Nakayama, K. Yamasu
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Ginowan
  • Year and Date: 2011-05-19
• [Presentation] Functional alteration of the Gbx2 transcription factor during zebrafish brain development (2011)
  • Author(s): Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta & Kyo Yamasu
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター(沖縄)
• [Presentation] Class III POU factors directly regulate Otx2 expression by binding to the forebrain and midbrain enhancer (2011)
  • Author(s): Fumitaka Inoue, Maiko Takahashi, Daisuke Kurokawa, Nobuyoshi Takasaki, Tomomi Ohmura, Kyo Yamasu, Shinichi Aizawa
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター(沖縄)
• [Presentation] Requirements of a Class V POU transcription factor Pou2/Pou5f1 in different aspects of zebrafish development (2011)
  • Author(s): Alam Khan, Kazumasa Takemoto, Hiroyuki Takeda, Yamasu, K
  • Organizer: The 5th Asia-Oceania Zebrafish Meeting
  • Place of Presentation: Beijing, China
• [Presentation] Molecular analysis of the Gbx2 function in the MHB formation in zebrafish embryo (2011)
  • Author(s): Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta, Kyo Yamasu
  • Organizer: 17th Japanese Medaka Zebrafish Meeting
  • Place of Presentation: 東レ総合研修センター（静岡）
• [Presentation] Analysis of the roles played by a class V POU protein, Pou2/Pou5f1, in zebrafish embryogenesis (2011)
  • Author(s): Alam Khan, Andrew Nakamoto, Shun Okamoto, Miyako Tai, Shinji Saito, Kazumasa Takemoto, Hiroyuki Takeda, Kyo Yamasu
  • Organizer: 17th Japanese Medaka Zebrafish Meeting
  • Place of Presentation: 東レ総合研修センター（静岡）
• [Presentation] Comprehensive analysis of the regulatory gene network initiated by gbx2 at the midbrain-hindbrain boundary (2011)
  • Author(s): Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta, Kyo Yamasu
  • Organizer: The 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: パシフィコ横山（神奈川）
• [Presentation] 小型魚類を用いた脳形成制御機構の発生遺伝学的研究 (2011)
  • Author(s): 弥益 恭
  • Organizer: 第10回国際バイオEXPO・バイオ アカデミックフォーラム
  • Place of Presentation: 東京ビッグサイト（東京）
• [Presentation] 脊椎動物胚における脳形成制御機構の研究：発生遺伝学的アプローチ (2011)
  • Author(s): 弥益 恭
  • Organizer: 自治医科大学＆埼玉大学・連携記念シンポジウム
  • Place of Presentation: 自治医科大学（栃木）
• [Remarks]
  • URL: http://devbiol.seitai.saitama-u.ac.jp
• [Remarks] 埼玉大学大学院理工学研究科 生命科学部門 理学部生体制御学科 発生生物学研究室
  • URL: http://devbiol.seitai.saitama-u.ac.jp
• [Remarks] 発生生物学研究室
  • URL: http://devbiol.seitai.saitama-u.ac.jp/index.html
• [Remarks] 埼玉大学 研究機構 脳科学融合研究センター
  • URL: http://subsi.saitama-u.ac.jp
• [Remarks]
  • URL: http://devbiol.seitai.saitama-u.ac.jp/