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Mechanism of the development of local organizers in the brain primordium using zebrafish as a model system

Research Project

Project/Area Number 23570247
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionSaitama University

Principal Investigator

YAMASU Kyo  埼玉大学, 大学院理工学研究科, 教授 (60230439)

Co-Investigator(Kenkyū-buntansha) KAWAMURA Akinori  埼玉大学, 大学院理工学研究科, 講師 (10466691)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords脳形成 / 脳部域化 / 遺伝子制御ネットワーク / ゼブラフィッシュ / 発生遺伝学 / 局所オーガナイザー / 転写制御機構 / 神経分化 / 遺伝子ネットワーク / 転写制御 / エンハンサー解析 / 転写調節因子 / GAL4-UAS系 / マイクロアレイ / 遺伝子制御
Research Abstract

We studied the regulatory mechanism of vertebrate brain formation in zebrafish. We revealed the details of the function of two isthmus-forming genes, gbx2 and pou2, suggesting that both are involved in neurogenesis. We also extended the analysis on the transcriptional regulation of an ANB-forming gene, emx3, and two isthmus-forming genes, pax2a and pou2. Meanwhile, it was shown that the development of the neural crest and somite is affected in a novel mutation, kanazutsi, and its causative gene encodes a globin-family protein. In addition, we succeeded in introducing two novel experimental techniques, the GAL4/UAS system and TALEN.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (54 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (44 results) (of which Invited: 2 results) Remarks (5 results)

  • [Journal Article] Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner2013

    • Author(s)
      Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K.
    • Journal Title

      Mechanisms of Development

      Volume: 130 Issue: 11-12 Pages: 532-552

    • DOI

      10.1016/j.mod.2013.07.004

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages2012

    • Author(s)
      Khan A, Nakamoto A, Okamoto S, Tai M, Nakayama Y, Kobayashi K, Kawamura A, Takeda H, Yamasu K.
    • Journal Title

      Mechasmims of Development

      Volume: 129 Issue: 9-12 Pages: 219-235

    • DOI

      10.1016/j.mod.2012.07.007

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafish embryogenesis.2012

    • Author(s)
      Khan A, Nakamoto A, Tai M, Saito S, Nakayama Y, Kawamura A, Takeda H, Yamasu K.
    • Journal Title

      Development, Growth & Differentiation

      Volume: 54 Issue: 7 Pages: 686-701

    • DOI

      10.1111/j.1440-169x.2012.01369.x

    • NAID

      10031136935

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Binding properties of thyroxine to nuclear extract from sea urchin larvae2012

    • Author(s)
      M Saito, K Yamasu, T Suyemitsu
    • Journal Title

      Zool

      Volume: 29 Issue: 2 Pages: 79-82

    • DOI

      10.2108/zsj.29.79

    • NAID

      40019149484

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Retinoic acid-dependent establishment of positional information in the hindbrain was conserved during vertebrate evolution2011

    • Author(s)
      A Ishioka, T Jindo, T Kawanabe, K Hatta, M S Parvin, M Nikaido, Y Kuroyanagi, H Takeda, K Yamasu
    • Journal Title

      Dev Biol

      Volume: 350 Issue: 1 Pages: 154-168

    • DOI

      10.1016/j.ydbio.2010.10.011

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] A novel mutation involved in the development neural crest cells and somites in zebrafish embryos2013

    • Author(s)
      K. Takahashi, K. Yamasu
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Gbx2 regulates neurogenesis in the cerebellum during early vertebrate development2013

    • Author(s)
      Y. Nakayama, K. Yamasu
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] In vitro analysis of the transcriptional regulatory mechanism of zebrafish pou2 that encodes a class V POU transcription factor2013

    • Author(s)
      K Kobayashi, K Yamasu
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Repeated utilization of Pou2, a class V POU-type transcription factor in zebrafish, in regulation of multiple aspects of early development2013

    • Author(s)
      A. Khan, K. Yamasu
    • Organizer
      8th European Zebrafish Meeting
    • Place of Presentation
      Barcelona Spain
    • Year and Date
      2013-07-11
    • Related Report
      2013 Final Research Report
  • [Presentation] A novel mutation involved in neural crest differentiation in zebrafish embryos2013

    • Author(s)
      K. Takahashi, K. Yamasu
    • Organizer
      8th European Zebrafish Meeting
    • Place of Presentation
      Barcelona Spain
    • Year and Date
      2013-07-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Studies on the gene network with a focus on Gbx2 transcription factor at the midbrain-hindbrain boundary2013

    • Author(s)
      Y. Nakayama, K. Yamasu
    • Organizer
      8th European Zebrafish Meeting
    • Place of Presentation
      Barcelona Spain
    • Year and Date
      2013-07-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Gbx2 regulates neurogenesis in the cerebellum during early vertebrate development2013

    • Author(s)
      Yukiko Nakayama, Kana Kobayashi, Zhe Wang, Kyo Yamasu
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] A novel mutation involved in the development neural crest cells and somites in zebrafish embryos2013

    • Author(s)
      Kazuki Takahashi, Yuki Ito, Mami Yoshimura, Masataka Nikaido, Akinori Kawamura, Kyo Yamasu
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] In vitro analysis of the transcriptional regulatory mechanism of zebrafish pou2 that encodes a class V POU transcription factor2013

    • Author(s)
      Kana Kobayashi, Alam Khan, Kyo Yamasu
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Transcriptional regulation of hes6 gene in presomitic mesoderm and tailbud in zebrafish2013

    • Author(s)
      Akinori Kawamura, Miki Hoshikawa, Kenji Nakajo, Hiroki Ovara, Daisuke Yokota, Shin-ichiHigashijima, Shinji Takada, Kyo Yamasu
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comprehensive analysis of gbx2 target genes suggests that gbx2 functions as a transcriptional activator or a repressor depending on tissues and target genes2013

    • Author(s)
      Yukiko Nakayama, Zhe Wang, Kana Kobayashi, Kyo Yamasu
    • Organizer
      第19回 小型魚類研究会
    • Place of Presentation
      仙台市情報・産業プラザ(宮城県仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Transcriptional regulatory mechanism of pou2 that controls a broad spectrum of zebrafish development2013

    • Author(s)
      Kana Kobayashi, Alam Khan, Mst. Shahnaj Parvin, Kyo Yamasu
    • Organizer
      第19回 小型魚類研究会
    • Place of Presentation
      仙台市情報・産業プラザ(宮城県仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] A novel mutation involved in the development of neural crest-derived tissues and organs in zebrafish embryos2013

    • Author(s)
      Yuki Ito, Kazuki Takahashi, Mami Yoshimura, Masataka Nikaido, Akinori Kawamura, Kyo Yamasu
    • Organizer
      第19回 小型魚類研究会
    • Place of Presentation
      仙台市情報・産業プラザ(宮城県仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] A cis-regulatory module downstream of hes6 gene controlled by T-box and Ets transcription factors drives the expression in presomitic mesoderm and tailbud of zebrafish embryos2013

    • Author(s)
      Akinori Kawamura, Miki Hoshikawa, Kenji Nakajo, Hiroki Ovara, Daisuke Yokota, Shin-ichi Higashijima, Shinji Takada, Kyo Yamasu
    • Organizer
      第19回 小型魚類研究会
    • Place of Presentation
      仙台市情報・産業プラザ(宮城県仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Repeated utilization of Pou2, a class V POU-type transcription factor in zebrafish, in regulation of multiple aspects of early development2013

    • Author(s)
      Alam Khan, Andrew Nakamoto, Yukiko Nakayama, Kana Kobayashi, Akinori Kawamura, Hiroyuki Takeda, and Kyo Yamasu
    • Organizer
      8th European Zebrafish Meeting
    • Place of Presentation
      Palau de Congressos de Catalunya (Barcelona, Spain)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Studies on the gene network with a focus on Gbx2 transcription factor at the midbrain-hindbrain boundary2013

    • Author(s)
      Yukiko Nakayama, Zhe Wang, Kana Kobayashi, Hiroshi Kikuta, Kyo Yamasu
    • Organizer
      8th European Zebrafish Meeting
    • Place of Presentation
      Palau de Congressos de Catalunya (Barcelona, Spain)
    • Related Report
      2013 Annual Research Report
  • [Presentation] A novel mutation involved in neural crest differentiation in zebrafish embryos2013

    • Author(s)
      Yuki Ito, Kazuki Takahashi, Mami Yoshimura, Masataka Nikaido, Akinori Kawamura, Kyo Yamasu
    • Organizer
      8th European Zebrafish Meeting
    • Place of Presentation
      Palau de Congressos de Catalunya (Barcelona, Spain)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Functional analysis of the forebrain-forming genes in zebrafish embryos by the GAL4-UAS system2012

    • Author(s)
      H. Taira, K. Yamasu
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of the telencephalon enhancer of emx3 that functions in zebrafish embryos2012

    • Author(s)
      K. Shimura, K. Yamasu
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of zebrafish Gbx2 as a transcriptional regulator in embryos and cell culture system2012

    • Author(s)
      Y. Nakayama, K. Yamasu
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of Gbx2 in brain development using the heat shock induction system2012

    • Author(s)
      Y. Nakayama, K. Yamasu
    • Organizer
      10th International Conference on Zebrafish Development & Genetics
    • Place of Presentation
      Madison USA
    • Year and Date
      2012-06-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of the roles played by a zebrafish class V POU protein Pou2/Pou5f1 in mesendodermal specification2012

    • Author(s)
      A. Khan, K. Yamasu
    • Organizer
      45th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Kobe
    • Year and Date
      2012-05-31
    • Related Report
      2013 Final Research Report
  • [Presentation] The Gbx2 homeodomain protein functions as a transcriptional repressor in zebrafish embryos and embryonal carcinoma cells2012

    • Author(s)
      Y. Nakayama, K. Yamasu
    • Organizer
      45th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Kobe
    • Year and Date
      2012-05-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of the roles played by a zebrafish class V POU protein, Pou2/Pou5f1, in mesendodermal specification2012

    • Author(s)
      Alam Khan, Yukiko Nakayama & Kyo Yamasu
    • Organizer
      日本発生生物学会第45回大会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2012 Research-status Report
  • [Presentation] The Gbx2 homeodomain protein functions as a transcriptional repressor in zebrafish embryos and embryonal carcinoma cells2012

    • Author(s)
      Yukiko Nakayama, Kimihito Yoshikawa, Zhe Wang, Maiko Kanai, and Kyo Yamasu
    • Organizer
      日本発生生物学会第45回大会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2012 Research-status Report
  • [Presentation] Functional analysis of Gbx2 in brain development using the heat shock induction system2012

    • Author(s)
      Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta, Kyo Yamasu
    • Organizer
      10th International Conference on Zebrafish Development & Genetics
    • Place of Presentation
      University of Wisconsin (Madison, USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] Functional analysis of the forebrain-forming genes in zebrafish embryos by the Gal4-UAS system2012

    • Author(s)
      Hitoshi Taira, Yuri Kuroyanagi, Koichi Kawakami, and Kyo Yamasu
    • Organizer
      第18回 小型魚類研究会
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] Gbx2 functions as a transcriptional activator in zebrafish embryos2012

    • Author(s)
      Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta, Kyo Yamasu
    • Organizer
      第18回 小型魚類研究会
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] 脊椎動物胚における脳形成制御機構の研究:発生遺伝学的アプローチ2012

    • Author(s)
      弥益 恭
    • Organizer
      第3回 埼玉大学脳科学融合研究センター・シンポジウム「脳の発生・発達とその破綻の理解をめざして」
    • Place of Presentation
      埼玉大学(さいたま)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Functional analysis of zebrafish Gbx2 as a transcriptional regulator in embryos and cell culture system2012

    • Author(s)
      Yukiko Nakayama, Zhe Wang, Kana Kobayashi, Hiroshi Kikuta, Maiko Kanai, and Kyo Yamasu
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] Identification of the telencephalon enhancer of emx3 that functions in zebrafish embryos2012

    • Author(s)
      Kyosuke Shimura, Yuri Kuroyanagi, Rieko Nakamura, Kyo Yamasu
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] Functional analysis of the forebrain-forming genes in zebrafish embryos by the GAL4-UAS system2012

    • Author(s)
      Hitoshi Taira, Ayano Shinoto, Gembu Abe, Koichi Kawakami, and Kyo Yamasu
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] 脊椎動物の発生において脳原基の部域化を制御する遺伝子ネットワーク2012

    • Author(s)
      弥益 恭
    • Organizer
      埼玉大学・国立環境研究所共催シンポジウム「我々を取り巻く環境と健康 ~環境科学と基礎生物学の最近の研究動向~」
    • Place of Presentation
      埼玉会館(さいたま)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Comprehensive analysis of the regulatory gene network initiated by gbx2 at the midbrain-hindbrain boundary2011

    • Author(s)
      Y. Nakayama, K. Yamasu
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Requirements of a Class V POU transcription factor Pou2/Pou5f1 in different aspects of zebrafish development2011

    • Author(s)
      A. Khan, K. Yamasu
    • Organizer
      The 5th Asia-Oceania Zebrafish Meeting
    • Place of Presentation
      Beijing China
    • Year and Date
      2011-08-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional alteration of the Gbx2 transcription factor during zebrafish brain development2011

    • Author(s)
      Y. Nakayama, K. Yamasu
    • Organizer
      44th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Ginowan
    • Year and Date
      2011-05-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional alteration of the Gbx2 transcription factor during zebrafish brain development2011

    • Author(s)
      Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta & Kyo Yamasu
    • Organizer
      44th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Related Report
      2011 Research-status Report
  • [Presentation] Class III POU factors directly regulate Otx2 expression by binding to the forebrain and midbrain enhancer2011

    • Author(s)
      Fumitaka Inoue, Maiko Takahashi, Daisuke Kurokawa, Nobuyoshi Takasaki, Tomomi Ohmura, Kyo Yamasu, Shinichi Aizawa
    • Organizer
      44th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Related Report
      2011 Research-status Report
  • [Presentation] Requirements of a Class V POU transcription factor Pou2/Pou5f1 in different aspects of zebrafish development2011

    • Author(s)
      Alam Khan, Kazumasa Takemoto, Hiroyuki Takeda, Yamasu, K
    • Organizer
      The 5th Asia-Oceania Zebrafish Meeting
    • Place of Presentation
      Beijing, China
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular analysis of the Gbx2 function in the MHB formation in zebrafish embryo2011

    • Author(s)
      Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta, Kyo Yamasu
    • Organizer
      17th Japanese Medaka Zebrafish Meeting
    • Place of Presentation
      東レ総合研修センター(静岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of the roles played by a class V POU protein, Pou2/Pou5f1, in zebrafish embryogenesis2011

    • Author(s)
      Alam Khan, Andrew Nakamoto, Shun Okamoto, Miyako Tai, Shinji Saito, Kazumasa Takemoto, Hiroyuki Takeda, Kyo Yamasu
    • Organizer
      17th Japanese Medaka Zebrafish Meeting
    • Place of Presentation
      東レ総合研修センター(静岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] Comprehensive analysis of the regulatory gene network initiated by gbx2 at the midbrain-hindbrain boundary2011

    • Author(s)
      Yukiko Nakayama, Zhe Wang, Maiko Kanai, Hiroshi Kikuta, Kyo Yamasu
    • Organizer
      The 34th Annual Meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      パシフィコ横山(神奈川)
    • Related Report
      2011 Research-status Report
  • [Presentation] 小型魚類を用いた脳形成制御機構の発生遺伝学的研究2011

    • Author(s)
      弥益 恭
    • Organizer
      第10回国際バイオEXPO・バイオ アカデミックフォーラム
    • Place of Presentation
      東京ビッグサイト(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] 脊椎動物胚における脳形成制御機構の研究:発生遺伝学的アプローチ2011

    • Author(s)
      弥益 恭
    • Organizer
      自治医科大学&埼玉大学・連携記念シンポジウム
    • Place of Presentation
      自治医科大学(栃木)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://devbiol.seitai.saitama-u.ac.jp

    • Related Report
      2013 Final Research Report
  • [Remarks] 埼玉大学大学院理工学研究科 生命科学部門 理学部生体制御学科 発生生物学研究室

    • URL

      http://devbiol.seitai.saitama-u.ac.jp

    • Related Report
      2013 Annual Research Report
  • [Remarks] 発生生物学研究室

    • URL

      http://devbiol.seitai.saitama-u.ac.jp/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks] 埼玉大学 研究機構 脳科学融合研究センター

    • URL

      http://subsi.saitama-u.ac.jp

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://devbiol.seitai.saitama-u.ac.jp/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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