| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Research Abstract |
The present study aimed to establish a long-term culture condition for zebrafish spermatogonial stem cells (SSCs) by novel growth factors, which facilitates genetic manipulation in SSCs. Microarray analysis and in vitro culture of SSCs revealed that platelet-derived growth factor, bone morphogenetic protein inhibitor, hyaluronic acid, and heparan sulfate have an effect on the proliferation of SSCs. In addition, the use of a hypertrophied testis enabled us to prolong the SSC culture length more than 2 months, and to transfect foreign genes efficiently. Furthermore, a hypertrophied testis were maintained continuously by subcutaneously grafting into immunedeficient zebrafish. These results will allow us to establish culture system to select genetically modified SSCs.
|
