Identification of growth factors for zebrafish spermatogonial stem cells and the utilization
| Project/Area Number |
23570260
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
SAKAI Noriyoshi 国立遺伝学研究所, 系統生物研究センター, 准教授 (50202081)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ゼブラフィッシュ / 精原幹細胞 / 精子形成 / 細胞培養 |
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| Research Abstract |
The present study aimed to establish a long-term culture condition for zebrafish spermatogonial stem cells (SSCs) by novel growth factors, which facilitates genetic manipulation in SSCs. Microarray analysis and in vitro culture of SSCs revealed that platelet-derived growth factor, bone morphogenetic protein inhibitor, hyaluronic acid, and heparan sulfate have an effect on the proliferation of SSCs. In addition, the use of a hypertrophied testis enabled us to prolong the SSC culture length more than 2 months, and to transfect foreign genes efficiently. Furthermore, a hypertrophied testis were maintained continuously by subcutaneously grafting into immunedeficient zebrafish. These results will allow us to establish culture system to select genetically modified SSCs.
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Report
(4 results)
Research Products
(15 results)
