Characterization of a Escherichia coli strain that evolved in an experimental coevolution system
| Project/Area Number |
23570268
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 実験進化学 / バクテリオファージ / 大腸菌 / 共進化 / 実験進化 / RNAバクテリオファージ / Ｆ繊毛 / 実験室内進化系 / 感染抵抗性 |
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| Research Abstract |
To examine the ongoing changes driven by host-parasite interactions, I have constructed a coevolution model consisting of Escherichia coli and the bacteriophage Qb. Whole genome analysis indicated that E.coli showed fixation of two mutations (in traQ anc csdA) faster than in sole E.coli experimental evolution.
To elucidate the mechanism underlying this coexistence, I investigated how a mutation in TraQ changed the physiological state of E. coli. Overexpression of wild-type TraQ in the partially resistant strain of E. coli caused both TraA protein content, including propilin and pilin, and Qb amplification to recover to levels comparable with observed in the susceptible strain. These results suggest the possibility that the mutation in TraQ may cause heterogeneity within the E. coli population, with a small number of cells with F pili supporting the phage population and a large number of cells without F pili supporting the E. coli population.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article]2012
Author(s)
Tomonori Inomata, Hitomi Kimura, Haruki Hayasaka, Akinori Shiozaki, Yasuhiro Fujita, and Akiko Kashiwagi
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Journal Title
Archives of Virology
Volume: Vol.157(11)
Pages: 2163-2169
Related Report
Peer Reviewed
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[Journal Article]2011
Author(s)
Saburo Tsuru, Nao Yasuda, Yoshie Murakami, Junya Ushioda, Kotaro Mori, Akiko Kashiwagi, Shingo Suzuki, Bei-Wen Ying, and Tetsuya Yomo
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Journal Title
Molecular Systems Biology
Volume: Vol.7
Pages: 493-493
Related Report
Peer Reviewed
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[Presentation]2013
Author(s)
柏木明子,菅原竜,熊谷知史,對馬(佐野)文恵,熊坂直也,四方哲也
Organizer
第65回日本生物工学会大会
Place of Presentation
広島国際会議場(広島県)
Related Report
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[Presentation]2013
Author(s)
柏木明子,菅原竜,對馬(佐野)文恵,熊谷知史,四方哲也
Organizer
日本進化学会第15回筑波大会
Place of Presentation
筑波大学(茨城県)
Related Report
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[Presentation]2012
Author(s)
菅原竜,對馬(佐野)文恵,熊谷知史,四方哲也,柏木明子
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場・マリンメッセ福岡(福岡県)
Related Report
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[Presentation] 独立栄養から従属栄養に向けてのシアノバクテリアの実験進化2012
Author(s)
羽渕真清, 宮崎美賀子, 瀧川剛, 松本佑介, 末吉眞人, 木内あや子, 細田一史, 鈴木真吾, 柏木明子, 森光太郎, 四方哲也
Organizer
日本進化学会第14回東京大会
Place of Presentation
首都大学東京
Related Report
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[Presentation]2011
Author(s)
菅原竜,四方哲也,柏木明子
Organizer
第34回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(神奈川県)
Related Report
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[Presentation]2011
Author(s)
柏木明子,四方哲也
Organizer
日本遺伝学会第83回大会
Place of Presentation
京都大学(京都府)
Related Report
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[Presentation]2011
Author(s)
柏木明子,四方哲也
Organizer
日本進化学会第13回大会
Place of Presentation
京都大学(京都府)
Related Report
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