Establishment of the haploid breeding method using kinetochore malfunction lines

• Project/Area Number: 23580007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Breeding science
• Research Institution: Okayama University
• Principal Investigator: NAGAKI Kiyotaka 岡山大学, 資源植物科学研究所, 准教授 (70305481)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 半数体 / 動原体

Research Abstract

In this study, I tried to establish the haploid breeding method using kinetochore malfunction lines. First, plants expressing GFP::CENH3 (centromere specific histone H3) were made by transformations using Arabidopsis, tobacco and tomato for the purpose. Next, RNAi constructs to knock down internal CENH3 were transformed to the plants. Some transformants having these constructs were isolated but expression levels of internal CENH3 in the transformants were as same as these in wild type. These results suggested that RNAi method is not sufficient for the purpose.