| Project/Area Number |
23580149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
MIZUTANI Masaharu 神戸大学, (連合)農学研究科(研究院), 准教授 (60303898)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIMOTO Yukihiro 神戸大学, 大学院農学研究科, 教授 (10243411)
UENO Kotomi 神戸大学, 大学院農学研究科, 術推進研 究員 (40582028)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ストリゴラクトン / コウモリカズラ / ストリゴール / GR24 / ソルガム / ソルゴモール / P450 / 5-デオキシストリゴール / ストライゴラクトン / 5-deoxystrigol / 重水素標識 / フロリドン / 培養根 |
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| Research Abstract |
Deuterium-labeled 5-deoxystrigol was synthesized, and the analytical method for strigolactones by UPLC-ESI-MS was established. When the root-cultures of Menispermum dauricum, which are a high producer of strigol, were fed with deuterium-labeled 5-deoxystrigol, the conversion of 5-deoxystrigol to strigol was not detected. This suggests that strigol is not biosynthesized via 5-deoxystrigol in Menispermum dauricum. On the other hand, GR24, a synthetic strigolactone analog, was metabolized to 7-OH-GR24 by the root cultures. Next, deuterium-labeled 5-deoxystrigol was administered to hydroponics of a high sorgomol-producing sorghum cultivar, Sorghum bicolor (L.) Moench, and conversion to sorgomol was investigated. 5-deoxystrigol was metabolized to sorgomol and the conversion was inhibited by a P450 inhibitor. These results suggest that sorgomol is biosynthesized from 5-deoxystrigol via a P450-dependent C-9 hydroxylation.
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