| Project/Area Number |
23580179
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAKI Toshiyuki 富山県立大学, 工学部, 教授 (70293909)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 機能性成分 / ポリフェノール / グルクロン酸抱合 / 硫酸抱合 / ＵＤＰ-グルクロン酸転移酵素 / 硫酸基転移酵素 / 国際情報交換、フィンランド / 国際情報交流、フィンランド |
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| Research Abstract |
In order to synthesize the conjugates as dietary metabolites, polyphenols, we have developed several mammalian UDP-glucuronosyltransferases (UGT) or sulfotransferase (SULT) expression systems in budding yeast. For the glucuronide production, mammalian UGT and UDP-glucose dehydrogenase (UGDH) were expressed in budding yeas using a multicopy and a genome integrated vectors. Using genetically engineered yeast containing UGT and UGDH, glucuronide formation of quercetin was examined. Querectin with multiple glucuronidating sites was conjugated as isoform-dependent formation using UGT isoforms. In the presence of glucose and ammonium sulfate, formation of sulfated quercetin was observed in whole-cell production system with SULT isoforms .These expression system of mammalian UGT or SULT in budding yeast would be a powerful tool for enzyme-assisted synthesis of various dietary metabolites including glucuronides and sulfates.
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