Whole cell-dependent production of polyphenol conjugates using genetically engineered budding yeast
Project/Area Number |
23580179
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Food science
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Research Institution | Toyama Prefectural University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SAKAKI Toshiyuki 富山県立大学, 工学部, 教授 (70293909)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 機能性成分 / ポリフェノール / グルクロン酸抱合 / 硫酸抱合 / UDP-グルクロン酸転移酵素 / 硫酸基転移酵素 / 国際情報交換、フィンランド / 国際情報交流、フィンランド |
Research Abstract |
In order to synthesize the conjugates as dietary metabolites, polyphenols, we have developed several mammalian UDP-glucuronosyltransferases (UGT) or sulfotransferase (SULT) expression systems in budding yeast. For the glucuronide production, mammalian UGT and UDP-glucose dehydrogenase (UGDH) were expressed in budding yeas using a multicopy and a genome integrated vectors. Using genetically engineered yeast containing UGT and UGDH, glucuronide formation of quercetin was examined. Querectin with multiple glucuronidating sites was conjugated as isoform-dependent formation using UGT isoforms. In the presence of glucose and ammonium sulfate, formation of sulfated quercetin was observed in whole-cell production system with SULT isoforms .These expression system of mammalian UGT or SULT in budding yeast would be a powerful tool for enzyme-assisted synthesis of various dietary metabolites including glucuronides and sulfates.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] A Possible Mechanism for 2,3',4,4',5'-Pentachlorobiphenyl-Mediated Decrease in Serum Thyroxine Level in Mice2013
Author(s)
Kato Y, Onishi M, Haraguchi K, Ikushiro S, Ohta C, Koga N, Endo T, Yamada S, and Degawa M
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Journal Title
Biol. Pharm. Bull.
Volume: 36
Pages: 1594-1601
NAID
Related Report
Peer Reviewed
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[Journal Article] Comparative Study on 2,2',4,5,5'-Pentachlorobiphenyl-Mediated Decrease in Serum Thyroxine Level between C57BL/6 and Its Transthyretin-Deficient Mice.2012
Author(s)
Kato Y., Tamaki S., Haraguchi K.,Ikushiro S., Sekimoto M., Ohta C., Endo T., Koga N., Yamada S., and Degawa M.
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Journal Title
Toxicology and Applied Pharmacology
Volume: 263
Pages: 323-329
Related Report
Peer Reviewed
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[Journal Article] Multiple isoform dependent glucuronidation of a selective c-Fos/activator protein-1 inhibitor T-52242011
Author(s)
Uchihashi, S.,Fukumoto, H., Onoda, M., Hayakawa, H., Ikushiro, S., and Sakaki, T.
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Journal Title
Drug Metabolism and Disposition
Volume: 39
Pages: 803-813
Related Report
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[Journal Article] Multiple isoform dependent glucuronidation of a selective c-Fos/activator protein-1 inhibitor T-5224.2011
Author(s)
Uchihashi, S.,Fukumoto, H., Onoda, M., Hayakawa, H., Ikushiro, S., and Sakaki, T.
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Journal Title
Drug Metabolism and Disposition
Volume: 39
Pages: 803-813
Related Report
Peer Reviewed
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[Journal Article] A Possible Mechanism for 2,2',4,4',5,5'-Hexachlorobiphenyl- Mediated Decrease in Serum Thyroxine Level in Mice.2011
Author(s)
Kato, Y., Onishi, M., Haraguchi, K., Ikushiro, S., Ota, C., Koga, N., Endo, T., Yamada, S., and Degawa, M.
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Journal Title
Toxicology and Applied Pharmacology
Volume: 254
Pages: 48-55
Related Report
Peer Reviewed
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[Journal Article] The impact of pharmacogenetics of metabolic enzymes and transporters on the pharmacokinetics of telmisartan in healthy volunteers2011
Author(s)
Yamada, A., Maeda, K., Ishiguro, N., Tsuda, Y., Igarashi, T., Ebner, T., Roth, W., Ikushiro, S., and Sugiyama, Y.
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Journal Title
Pharmacogenetics and Genomics
Volume: 21
Pages: 523-530
Related Report
Peer Reviewed
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[Journal Article] UDP-Glucuronosyltransferase 2B15 Polymorphism on Bisphenol A Glucuronidation.2011
Author(s)
Hanioka, N., Hiroyuki, Oka, H., Nagaoka, K., Ikushiro, S., Narimatsu, S.
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Journal Title
Archives of Toxicology
Volume: 85
Pages: 1378-1381
Related Report
Peer Reviewed
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