Studies on mecahnisms and application of carbon dioxide petite high pressure pasteurization technology.

• Project/Area Number: 23580191
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Gifu University
• Principal Investigator: IWAHASHI Hitoshi 岐阜大学, 応用生物科学部, 教授 (60356540)
• Co-Investigator(Renkei-kenkyūsha): MASATAKA Kusube 和歌山工業高等専門学校, 物質工学科, 准教授 (40403761)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 食品製造・加工 / 殺菌 / 炭酸ガス / OMICS解析 / 殺菌技術 / 酵母 / ゲノミクス / メタボロミクス / 食品

Research Abstract

Carbon dioxide petite high pressure pasteurization technology was applied to Umeboshi, fruit deuce, non-pasteurized Sake, fermented fish sauce, and so on. These pasteurization can be carried out under less than 1 MPa, corresponding to upper range restricted by Japanese law. However, the mechanisms how microbes pasteurized or the ranges for applicable microbes are not yet well studied. Thus, this project aimed to study 1) genomics and metabolomics analysis of Saccharomyces cerevisiae under carbon dioxide petite high pressure conditions and 2) pasteurization profiling of applicable microbes using microbial type culture collections. With these studies fundamental information concern to carbon dioxide petite high pressure pasteurization will be accumulated.

Research Products

• [Journal Article] Pressure regulated fermentation : A revolutionary approach that utilizes hydrostatic pressure Reviews in Agricultural (2014)
  • Author(s): Nomura, K., Iwahashi, H.
  • Journal Title: Science Volume: 2 Pages: 1-10
• [Journal Article] Pressure regulated fermentation: A revolutionary approach that utilizes hydrostatic pressure (2014)
  • Author(s): Nomura, K., Iwahashi, H.
  • Journal Title: Reviews in Agricultural Science Volume: 2 Pages: 1-10
  • NAID: 130007627510
  • Peer Reviewed
• [Journal Article] Environmental OMICS : Current Status and Future Directions (2013)
  • Author(s): Ge, Y., Wang, D.Z. Chiu, J.F., Cristobal, S., Sheehan, D., Silvestre, F., Peng,X., Li, H., Gong, Z., Lam S.H., Wentao, H., Iwahashi, H., Liu, J., Mei,N., Shi,L., Bruno, M., Foth, H., Teichman, K.
  • Journal Title: J. Int. OMICS Volume: 141 Pages: 75-87
• [Journal Article] Clarification of the recovery mechanism of Escherichia coli after hydrostatic pressure treatment (2013)
  • Author(s): Ohshima, S., Nomura, K., Iwahashi, H.
  • Journal Title: High Pressure Research Volume: 33:2 Pages: 308-314
• [Journal Article] 高圧力条件下における酵母細胞の死 (2013)
  • Author(s): 野村一樹、岩橋均
  • Journal Title: 高圧力の科学と技術 Volume: 23 Pages: 53-58
  • NAID: 10031156148
• [Journal Article] Clarification of the recovery mechanism of Escherichia coli after hydrostatic pressure treatment. (2013)
  • Author(s): 188. Ohshima, S., Nomura, K., Iwahashi, H.
  • Journal Title: High Pressure Research Volume: 33 Pages: 308-314
• [Journal Article] 高圧力条件下における酵母細胞の死 (2012)
  • Author(s): 岩橋 均
  • Journal Title: 高圧力学会誌 Volume: 印刷中
  • NAID: 10031156148
  • Peer Reviewed
• [Presentation] 3A12p07酵母を指標とした微高圧炭酸ガスストレス応答の解析 (2014)
  • Author(s): 貝沼瑞生,牛力源,野村一樹,岩橋均,松岡寛之,河内哲史,鈴木良尚,田村勝弘
  • Organizer: 2014年度農芸化学会大会
  • Place of Presentation: 東京,日本
• [Presentation] 酵母を指標とした微高圧炭酸ガスストレス応答の解析 (2014)
  • Author(s): 貝沼瑞生、牛 力源、野村一樹、岩橋 均、松岡寛之、河内哲史'、鈴木良尚、田村勝弘
  • Organizer: 日本農芸化学会
  • Place of Presentation: 明治大学
• [Presentation] 遺伝子発現プロファイル解析により示されたSaccharomyces cerevisiaeにおける圧力感受性と呼吸欠損の関連性. (2013)
  • Author(s): 野村 一樹, 岩橋 均, 井口 晃徳, 重松 亨
  • Organizer: 日本農芸化学会2013年次大会
  • Place of Presentation: 仙台
• [Presentation] The development of liquid feeding with petit-high pressure carbon dioxide gas (2012)
  • Author(s): K. Hachisuka, K. Nomura, S. Waki and H. Iwahashi
  • Organizer: HPBB2012 p. 129
  • Place of Presentation: Shiga, Japan
• [Presentation] Proposal of preparation protocols for indicator microorganisms aimed to standardizing high-pressure processing technology (2012)
  • Author(s): L. Niu, K. Nomura, H. Iwahashi, K. Obuchi, M. Kawamura, A. Kobayashi, A.Yamasaki, Y. Batori, Y. Kasai, H. Urakami
  • Organizer: HPBB2012 p. 132
  • Place of Presentation: Shiga, Japan
• [Presentation] Clarification of the recovery mechanism after hydrostatic pressure treatment in Escherichia coli (2012)
  • Author(s): S. Ohshima, K. Nomura, and H.Iwahashi
  • Organizer: HPBB2012 p. 134
  • Place of Presentation: Shiga, Japan
• [Presentation] The effect of carbon dioxide to yeast Saccharomyces cerevisiae (2012)
  • Author(s): S. Waki, Y. Hachisuka, K. Nomura, H. Iwahashi, H. Matsuoka, S. Kawachi, Y. Suzuki, K. Tamura
  • Organizer: HPBB2012 p. 139
  • Place of Presentation: Shiga, Japan
• [Presentation] Biological reason why yeast is restored from temporal deathafter sub-lethal high pressure treatment- Primary damage - (2012)
  • Author(s): H. Iwahashi
  • Organizer: HPBB2012 p. 136
  • Place of Presentation: Shiga, Japan
• [Presentation] he effect of carbon dioxide to yeast Saccharomyces cerevisiae. (2012)
  • Author(s): Shiori Waki, Yoshihiko Hachisuka, Kazuki Nomura, Hitoshi Iwahashi, Hiroyuki Matsuoka, Satoshi Kawachi, Yoshihisa Suzuki, Katsuhiro Tamura
  • Organizer: 7th International Conference on High Pressure Bioscience and Biotechnology,
  • Place of Presentation: 滋賀
• [Presentation] 高圧力条件下における酵母細胞の死 (2012)
  • Author(s): 岩橋 均
  • Organizer: 高圧力学会(招待講演)
  • Place of Presentation: 京都市
• [Presentation] 炭酸ガス殺菌のメカニズム (2011)
  • Author(s): 岩橋 均
  • Organizer: 生物領域高圧研究会
  • Place of Presentation: 新潟市
• [Book] 進化する食品高圧加工技術酵母への高圧力の影響―致死以上の圧力レベルを中心に―酵母への高圧力の影響 (2013)
  • Author(s): 野村一樹,大島秀斗,蜂須賀勝彦,水野陽太,牛力源,岩橋均
• [Book] 株式会社エヌ・ティー・エス (2013)
  • Author(s): 野村 一樹, 大島 秀斗, 蜂須賀 勝彦, 水野 陽太, 牛 力源, 岩橋 均
  • Total Pages: 10
  • Publisher: 食品分野における高圧力処理技術動向
• [Remarks]
  • URL: http://www.abios.gifu-u.ac.jp/h1884/