Taxonomic determination of kuchijirosho virus by genomic analysis.
Project/Area Number |
23580261
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General fisheries
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Research Institution | Fukui Prefectural University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SUETAKE Hiroaki 福井県立大学, 海洋生物資源学部, 准教授 (00334326)
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Co-Investigator(Renkei-kenkyūsha) |
ISSHIKI Tadashi 三重大学, 大学院生物資源学研究科, 准教 授 (30378319)
KITAMURA Shin-ichi 愛媛大学, 沿岸環境科学研究センター, 准 教授 (40448379)
TAKEUCHI Kenji 福井大学, 医学部, 助教(学内講師) (40236419)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | ウイルス / トラフグ / 口白症 / 魚病 / ゲノム解析 / 口白症ウイルス |
Research Abstract |
Kuchijirosho virus infects cultured fugu (Takifugu rubripes) and occured epizootic outbreaks in aquaculture farm in Japan. Physicochemical characterization and electron microscopic observation suggested that kuchijiroshou virus can belong to Genus Flavivirus, or at least Flaviviridae. We tried to RT-PCR amplification using 20 sets of PCR primer which are universal to Flavivirus, Hepacivirus, or Pestivirus; however, any virus-specific nucleotide sequence was amplified. "Rapid Determination System for Viral RNA Sequences" method was adopted to determine unknown virus genome nucleotide sequence. We succeeded first to clone 3 cDNA fragments from brains of kuchijirosho-infected fugu. Although These RNAs appeared in only kuchijirosho-affected brain, it is not clear that these RNAs are derived from kuchijirosho genome itself. So, we call these RNAs "kuchijirosho-assochiated RNA, KAR". To determine nucleotide sequence of whole genome, we are now perfoming next generation sequencer.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] A Trans-species Missense SNP in Amhr2 is Associated with Sex Determination in the Tiger Pufferfish, Takifugu rubripes (fugu)2012
Author(s)
Kamiya, T., Kai, W., Tasumi, S., Oka, A., Matsunaga, T., Mizuno, N., Fujita, M., Suetake, H., Suzuki, S., Hosoya, S., Tohari, S., Brenner, S., Miyadai, T., Venkatesh, B., Suzuki, Y., and Kikuchi, K.
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Journal Title
PLoS Genet
Volume: 8
Issue: 7
Pages: e1002798-e1002798
DOI
Related Report
Peer Reviewed
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[Journal Article] The plasmablast-like leukocyte in the kidney of fugu (Takifugu rubripes)2011
Author(s)
Odaka,T., Tsutsui,S., Sugamata,R., Suetake,H., Miyadai,T., Suzuki,Y., Watanabe,T., and Nakamura,O.
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Journal Title
Fish Shellfish Immunol
Volume: 30
Issue: 2
Pages: 682-690
DOI
Related Report
Peer Reviewed
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[Presentation] N-acetyl-d-glucosamine-binding immunoglobulin m of fugu (takifugu rubripes) inhibits the growth of fish pathogenic bacteria: a novel function of teleost antibody.2013
Author(s)
Shigeyuki Tsutsui, Toru Ariji, Akira Sato, Takaya Yoshida, Nozomi Yamamura, Tomoyuki Odaka, Kyosuke Araki, Hiroaki Suetake, Toshiaki Miyadai, Osamu Nakamura
Organizer
First International Conference of Fish and Shellfish Immunology
Place of Presentation
Vigo, Spain.
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