Research Project
Grant-in-Aid for Scientific Research (C)
Kuchijirosho virus infects cultured fugu (Takifugu rubripes) and occured epizootic outbreaks in aquaculture farm in Japan. Physicochemical characterization and electron microscopic observation suggested that kuchijiroshou virus can belong to Genus Flavivirus, or at least Flaviviridae.We tried to RT-PCR amplification using 20 sets of PCR primer which are universal to Flavivirus, Hepacivirus, or Pestivirus; however, any virus-specific nucleotide sequence was amplified."Rapid Determination System for Viral RNA Sequences" method was adopted to determine unknown virus genome nucleotide sequence. We succeeded first to clone 3 cDNA fragments from brains of kuchijirosho-infected fugu. Although These RNAs appeared in only kuchijirosho-affected brain, it is not clear that these RNAs are derived from kuchijirosho genome itself. So, we call these RNAs "kuchijirosho-assochiated RNA, KAR". To determine nucleotide sequence of whole genome, we are now perfoming next generation sequencer.
All 2014 2013 2012 2011
All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (34 results)
PLoS Genet
Volume: 8 Issue: 7 Pages: e1002798-e1002798
10.1371/journal.pgen.1002798
Developmental and Comparative Immunology
Volume: 38 (2) Issue: 2 Pages: 224-231
10.1016/j.dci.2012.02.002
Molecular Immunology
Volume: 48、Issues 6-7 Issue: 6-7 Pages: 818-825
10.1016/j.molimm.2010.10.018
Fish Shellfish Immunol
Volume: 30 Issue: 2 Pages: 682-690
10.1016/j.fsi.2010.12.018
Fisheries Science
Volume: 77(6) Issue: 6 Pages: 999-1007
10.1007/s12562-011-0405-0
10029654712
